Increasing the Effectiveness, Efficiency, and Specificity of Mutation Induction in Flowering Plants

Nilan, R.A.

doi:10.1007/978-1-4684-2880-3_14

Cited by 7 publications

(3 citation statements)

References 44 publications

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“…The highest frequency of viridis type was earlier reported by Yadav (1992) [9] and Gupta et al (2012) [8] in B. juncea. The reason for the appearance of greater number of viridis may be attributed to involvement of polygenes in the chlorophyll formation (Nilan and Konzak, 1961) [10] and the genes are probably more responsive to mutagen. Differential occurrence of mutations could be due to differential mode of action of mutagen on different base sequences in various genes.…”

Section: Resultsmentioning

confidence: 99%

Assessment of chlorophyll mutation in M2 generation of Indian mustard (Brassica juncea L. Czern and Coss)

Julia¹,

Sastry²,

Brajendra³

et al. 2022

Pharma Innovation

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The experiment was carried out during rabi 2018-19 and rabi 19-20 in the experimental field of College of Agriculture, Central Agricultural University, Imphal. Seeds of two genotypes of Indian mustard viz., CAULC-2 (local cultivar) and NRCHB-101 were exposed to three doses (1000, 1100 and 1200 Gy) of gamma rays, three concentrations of ethyl methanesulphonate (0.3, 0.5 and 0.7%) alone and in various combinations (1000Gy + 0.5%, 1100Gy + 0.5% and 1200Gy + 0.5%). Five different types of chlorophyll mutants viz., albina, chlorina, xantha, viridis and alboviridis were isolated from different treatments in M2 generation. Combination treatment of 1100 Gy+ 0.5% EMS produced the highest frequency of chlorophyll mutation in both the genotypes. Alboviridis and viridis types of mutants were more frequent in CAULC-2 and NRCHB-101 respectively, whereas albina type was least frequent in both the genotypes. CAULC-2 was more sensitive to mutagenic treatment as compared to NRCHB-101.

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Section: Resultsmentioning

confidence: 99%

Assessment of chlorophyll mutation in M2 generation of Indian mustard (Brassica juncea L. Czern and Coss)

Julia¹,

Sastry²,

Brajendra³

et al. 2022

Pharma Innovation

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“…Thus, an organic molecule that enters the nucleus, mingles with DNA, and causes point mutations in the genome facilitates SA mutagenicity. The hydrogen ion content of the treatment solution is the key to the high carcinogenic efficacy of SA, which is an inhibitor of the catalase and peroxidase enzymes, in barley (Nilan et al 1972). Additionally, it has been shown to result in mutations in Pisum (Sander and Muehlbauer 1977).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effects of Chemical Mutagens in Root Tip Cells of Allium cepa L.

Salim,

Bekhit,

Soudy

2023

Journal of Agricultural Chemistry and Biotechnology

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One of the excellent plant models for identifying various chromosomal abnormalities is Allium cepa L. In order to create genetic diversity, chemical mutagens such as; Ethyl methanesulfonate (EMS), Ethidium bromide (EtBr) and Sodium azide (SA) were used to mutagenize the plant's germplasm. The present study was carried out to determine how these chemicals affected the Mitotic Index (MI) and phase, chromosomal abnormalities, and frequency of micronuclei in the root tip cells of A. cepa Mutagens, in general, alter the genetic structure of the organisms, which is reflected in changes to a variety of features. Further, the genome's sensitivity was discovered through cytological examination when the three mutagens were used. The chromosomal entity was impacted by a variety of clastogenic and nonclastogenic alterations, as well as mitodepressive effects. The increase in dose/concentration of all the mutagens was shown to increase the frequency of both mitotic aberrant cells and mitotic chromosomal abnormalities in the treated root tip cells. Both chemical mutagens were shown to be less effective than EtBr at disrupting mitotic cell activity and primarily causing clastogenic chromosomal abnormalities. Numerous chromosomal aberrations, including stickiness and clumping of chromosomes with varying frequencies, fragments, laggards, bridges, precocious movements, micronuclei formation at telophases, and multipolar anaphases and telophases, were reported to be present in the mitotic aberrant cells. Anaphases, telophases, disorganised metaphases, desynchronized metaphases, and persisting nucleoli during anaphase were all noted.

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“…In whole plants, the mutation spectrum caused by certain mutagens, mutagen specificity, and efficiency of mutagens have been studied extensively (Brock, I969;Doll and Sandfaer, 1969;Gustafsson, I969;Kawal, 19695 Mikaelson, I969;Narayanan and Konzak, I969;Wlckham, Narayanan, and Konzak, I969» Ehrenberg, 1971;Gaul, Frimmel, Glchner, and Ulonska, 1972;Nilan, 1973;Auerbach, 1976).…”

Section: A Genetic and Epigenetic Variabilitymentioning

confidence: 99%

On the development of a mutant isolation scheme from cell and tissue cultures of Datura innoxia (Miller)

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Increasing the Effectiveness, Efficiency, and Specificity of Mutation Induction in Flowering Plants

Cited by 7 publications

References 44 publications

Assessment of chlorophyll mutation in M2 generation of Indian mustard (Brassica juncea L. Czern and Coss)

Assessment of chlorophyll mutation in M2 generation of Indian mustard (Brassica juncea L. Czern and Coss)

Cytotoxic Effects of Chemical Mutagens in Root Tip Cells of Allium cepa L.

On the development of a mutant isolation scheme from cell and tissue cultures of Datura innoxia (Miller)

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