Increasing the confidence in half-sibship determination based upon 15 STR loci

Pu, Chang En; Linacre, Adrian

doi:10.1016/j.jflm.2008.02.004

Cited by 12 publications

(12 citation statements)

References 6 publications

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“…These results indicated that the 23 autosomal STR loci included DNATyper25 are highly polymorphic and informative in the Han population of China. A number of studies have shown that the 13 core STR loci are insufficient for interpreting some difficult cases with close relatives. We hereby suggested that the non‐CODIS category could be used to resolve such cases.…”

Section: Resultsmentioning

confidence: 99%

Development of a new 25plex STRs typing system for forensic application

et al. 2019

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We have developed a novel STR 25‐plex florescence multiplex‐STR kit (DNATyper25) to genotype 23 autosomal and two sex‐linked loci for forensic applications and paternity analysis. Of the 23 autosomal loci, 20 are non‐CODIS. The sex‐linked markers include a Y‐STR locus (DYS391) and the Amelogenin gene. We present developmental validation studies to show that the DNATyper25 kit is reproducible, accurate, sensitive, and robust. Sensitivity testing showed that full profiles were achieved with as low as 125 pg of human DNA. Specificity testing demonstrated a lack of cross reactivity with a variety of commonly encountered non‐human DNA contaminants. Stability testing showed that full profiles were obtained with humic acid concentration ≤60 ng/μL and hematin concentration <400 μM. For forensic evaluation, the 23 autosomal STRs followed the Hardy–Weinberg equilibrium. In an analysis of 509 Chinese (CN) Hans, we detected a combined total of 181 alleles at the 23 autosomal STR loci. Since these autosomal STRs are independent from one another, PM was 8.4528 × 10−22, TDP was 0.999 999 999 999 999 999 999, CEP was 0.999 999 8395. The forensic efficiency parameters demonstrated that these autosomal STRs are highly polymorphic and informative in the Han population of China. We performed population comparisons and showed that the Northern CN Han has a close genetic relationship with the Luzhou Han, Tujia, and Bai populations. We propose that the DNATyper25 kit will be useful for cases where paternity analysis is difficult and for situations where DNA samples are limited in quantity and low in quality.

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Section: Resultsmentioning

confidence: 99%

Development of a new 25plex STRs typing system for forensic application

et al. 2019

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“…Specific cases may need to state multiple results to deal with different ethnic group assumptions, or address relatedness issues. Ongoing research into software applications and new marker panels is likely to increase the ability to clarify complicated and even remote biological relationships (Weir et al 2006;Pu and Linacre 2008;Lareu et al 2012). …”

Section: Discussionmentioning

confidence: 99%

Forensic DNA Analysis

Prinz¹,

Lessig²

2014

Handbook of Forensic Medicine

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“…However, challenges still exist in kinship analysis. A few of studies reveal that the routine forensic autosomal chromosome STR loci are not sufficient to discriminate between pairs of close relatives and unrelated individuals and may lead to false inclusions and exclusions . Even though 34 STR loci were used, distinguishing half‐sibs from either full‐sibs or unrelated pairs of individuals cannot be confirmed reliably .…”

Section: Introductionmentioning

confidence: 99%

Potential of 13 linked autosomal short tandem repeat loci in pairwise kinship analysis

Liu

Xue

et al. 2016

Electrophoresis

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In this study, a panel of 13 STR loci locate on chromosome 3, 4, and 17 (D3S2402, D3S2452, D3S1766, D3S4554, D3S2388, D3S3051, D3S3053, D4S2404, D4S2364, AC001348A, AC001348B, D17S975, and D17S1294) were assessed for pairwise kinship analysis. Map distances between these STR loci ranged from 0.07 cM to 97.03 cM. The population genetic study of Chinese Han population showed that linkage disequilibrium exists in two clusters of closely linked markers (D4S2404-D4S2364 and D17S975-D17S1294), in which the recombination fractions were 0.0026 and 0.0001, respectively. The recombination fractions derived from the Rutgers Map for the closely linked markers (genetic distance < 0.5 cM) were significant underestimates in comparison to those of direct observation of STR transmissions in families. When effect of linkage on pairwise kinship testing was evaluated by comparing likelihood ratio (LR) values taking linkage into account, overall LR values increased. But extremely low LRs were also observed. Finally, the power of the 13 STR loci to discriminate relationship among full-sibs, half-sibs, grandparent-grandchild, uncle-niece, and unrelated pairs was assessed with a category fraction. The results showed that about 72.64% of full-sib pairs and about 82.84% of unrelated pairs could be classified correctly. But the category fractions of second-degree relationships drastically reduced to 7.34-35.48%. If only pairs of grandparent-grandchild, half-sibs, and uncle-niece were distinguished, the category fraction was 0.5512, 0.1147, and 0.4362, respectively. Our study results demonstrated that linked STRs were helpful to differentiate the most frequent relationships in pairwise kinship analysis.

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Increasing the confidence in half-sibship determination based upon 15 STR loci

Cited by 12 publications

References 6 publications

Development of a new 25plex STRs typing system for forensic application

Development of a new 25plex STRs typing system for forensic application

Forensic DNA Analysis

Potential of 13 linked autosomal short tandem repeat loci in pairwise kinship analysis

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