Increasing the Complexity of Magnetic Core/Shell Structured Nanocomposites for Biological Applications

Salgueiriño, Verónica; Correa‐Duarte, Miguel A.

doi:10.1002/adma.200700418

Cited by 269 publications

(181 citation statements)

References 121 publications

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“…These include: magnetic resonance imaging (MRI), targeted drug delivery, detoxification of biological fluids, hyperthermia, catalysis, cell separation, cell labelling, cell targeting, biosensors, diagnostic medical devices, etc. [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization, and Toxicity Evaluation of Dextran-Coated Iron Oxide Nanoparticles

Balaș

Ciobanu

Burtéa

et al. 2017

Metals

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Abstract:We report the synthesis of dextran-coated iron oxide magnetic nanoparticles (DIO-NPs) with spherical shape and uniform size distribution as well as their accumulation and toxic effects on Jurkat cells up to 72 h. The characterization of dextran-coated maghemite nanoparticles was done by X-ray diffraction and dynamic light scattering analyses, transmission electron microscopy imaging, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, magnetic hysteresis, and relaxometry measurements. The quantification of DIO-NPs intracellular uptake showed a progressive accumulation of iron as a function of time and dose accompanied by additional lysosome formation and an increasing darkening exhibited by a magnetic resonance imaging (MRI) scanner. The cytotoxicity assays revealed a decrease of cell viability and a loss of membrane integrity in a time-and dose-dependent manner. Exposure to DIO-NPs determined an increase in reactive oxygen species level up to 72 h. In the first two days of exposure, the level of reduced glutathione decreased and the amount of malondyaldehyde increased, but at the end of the experiment, their concentrations returned to control values. These nanoparticles could be used as contrast agents for MRI but several parameters concerning their interaction with the cells should be taken into consideration for a safe utilization.

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Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization, and Toxicity Evaluation of Dextran-Coated Iron Oxide Nanoparticles

Balaș

Ciobanu

Burtéa

et al. 2017

Metals

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“…Therefore, indistinguishable X-ray absorption properties of these probes negate the possibility to utilize them in double labeling experiments with soft X-ray microscopy. With the rapidly expanding field of nanoscience in biology, especially the successful application of semiconductor nanocrystals in biological imaging, [4][5][6][7] additional nanoparticular materials that exhibit strong absorption in the soft X-ray spectrum are being developed. One promising candidate is TiO 2 .…”

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confidence: 99%

TiO2 nanoparticles as a soft X-ray molecular probe

Ashcroft

Zhang

et al. 2008

Chem. Commun.

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This communication reports the development of a TiO 2 -streptavidin nanoconjugate as a new biological label for X-ray bio-imaging applications; this new probe, used in conjunction with the nanogold probe, will make it possible to obtain quantitative, high-resolution information about the location of proteins using X-ray microscopy.Soft X-ray tomography generates 3D images of whole, hydrated cells at a resolution better than 50 nm. 1,2 High-contrast images of cellular structures are obtained because organic material absorbs roughly an order of magnitude more than water at this energy (517 eV). Minimal cell processing is required, as cells need only be frozen, and data collection is rapid (3-5 min per tomographic data set). X-Ray tomography is, therefore, an appealing imaging technique for those experiments that require better resolution than is possible with light microscopy. With light microscopy, fluorescent tags are routinely used to label molecules. For X-ray microscopy, we need probes that use the inherent X-ray properties and can specifically label proteins within the cellular environment. Since X-ray transmission is sensitive to absorbance and density differences in the specimen, as is transmission electron microscopy (TEM), probes used in TEM should also work for X-ray microscopy. Current approaches for localization used with TEM include labeling with Au nanoparticles or photooxidation of diaminobenzidene (DAB). Similar approaches have proven viable for soft X-ray microscopy, as demonstrated by the use of gold nanoparticles conjugated to antibodies to localize proteins in whole cells. 3Co-localization studies with light microscopy are routinely done using probes that fluoresce at two different wavelengths. For similar studies with X-ray microscopy, we need two tags that can be unambiguously identified, which means the probes need to have different absorption properties, such as an X-ray edge absorption. The dense DAB reaction product and the Au nanoparticles are directly visible in the soft X-ray microscope. However, the contrast mechanism of both probes is based on absorption density of the matter. No X-ray † Electronic supplementary information (ESI) edge absorption is available for either of them within the range of the operation energy. Therefore, indistinguishable X-ray absorption properties of these probes negate the possibility to utilize them in double labeling experiments with soft X-ray microscopy. With the rapidly expanding field of nanoscience in biology, especially the successful application of semiconductor nanocrystals in biological imaging, 4-7 additional nanoparticular materials that exhibit strong absorption in the soft X-ray spectrum are being developed. One promising candidate is TiO 2 . TiO 2 nanoparticles have been widely used in other industries due to their photocatalytic activity and UV light absorption properties. 8 They are also highly biocompatible. A recent study demonstrated the use of a TiO 2 -oligonucleotide nanocomposite as a unique light inducible nucleic acid e...

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“…Core/shell hybrid materials can be composed of different core materials, which are characterized by many interesting properties, such as: magnetic (Hyeon 2002;Deng et al 2008;Kim et al 2008), fluorescence (Tovmachenko et al 2006;Reiss et al 2009), luminescent etc. Especially, superparamagnetic iron oxide nanoparticles (SPIONs) (Salgueirino-Maceira and Correa-Duarte 2007) are promising materials for many biomedical applications, such as hyperthermia treatment , magnetic resonance imaging (MRI) (Schlorf et al 2011), targeted drug delivery systems, especially in cancer treatment Rosena et al 2012;Wahajuddin and Arora 2012), magnetically removable catalysts and, more recently, as magnetically removable adsorbents (Tang and Lo 2013;Zhang et al 2008). Nevertheless, there is a need to modify their surface in order to improve their biocompatibility and biodegradability.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, surface characterization and electrokinetic properties of colloidal silica nanoparticles with magnetic core

2016

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In order to obtain functionalized silica colloids with magnetic properties, the three-step reaction, was carried out. The aqueous suspension of iron oxide nanoparticles as magnetic core (maghemite-c-Fe 2 O 3 ) was synthesized according to the Massart's method (Massart in IEEE Trans Magn 17:1247-1248, 1981. A silica shell was obtained onto maghemite employing a modified sol-gel method by Salgueirino (Salgueirino-Maceira et al. in Adv Mater 19:4131-4144, 2006). As-synthesized magnetic silica core/shell nanocomposites were further functionalized by the post-grafting approach, using (3-Aminopropyl)-trimethoxysilane (APTMS) (Pham et al. in Langmuir 18:4915-4920, 2002). The obtained materials were characterized by nitrogen adsorption/desorption measurements, X-ray powder diffractometry (XRD), transmission electron microscopy (TEM). Particle sizes were measured by diffraction laser (DL). In order to establish colloidal stability of the system, zeta potential were measured. The syntheses routes led to obtain both, good purity of maghemite particles (more than 90 %) and their good coverage by outer silica shell. Thanks to their nanometer size and surface properties (especially, presence of amino groups which can ensure the electrostatic interaction between surface of adsorbent and adsorbate molecules), asprepared nanocomposites can be considered as promising

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Increasing the Complexity of Magnetic Core/Shell Structured Nanocomposites for Biological Applications

Cited by 269 publications

References 121 publications

Synthesis, Characterization, and Toxicity Evaluation of Dextran-Coated Iron Oxide Nanoparticles

Synthesis, Characterization, and Toxicity Evaluation of Dextran-Coated Iron Oxide Nanoparticles

TiO2 nanoparticles as a soft X-ray molecular probe

Synthesis, surface characterization and electrokinetic properties of colloidal silica nanoparticles with magnetic core

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