Increasing saugeye (S. vitreus × S. canadensis) production efficiency in a hatchery setting using assisted reproduction technologies

Blawut, Bryan; Wolfe, Barbara A.; Moraes, Christa R.; Ludsin, Stuart A.; Silva, Marco A. Coutinho da

doi:10.1016/j.aquaculture.2018.05.027

Cited by 9 publications

(6 citation statements)

References 24 publications

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“…Sperm concentrations were highest in the distal portion of the testis, intermediate in the middle section and lowest in the proximal portion of the testis and strip‐spawned spermatozoa (Table 1). Motility was significantly reduced in stripped spermatozoa compared with testicular spermatozoa but is most likely a result of urine contamination as they are not consistent with previous values for this species (Blawut et al., 2018; Blawut, Wolfe, Moraes, Ludsin, et al., 2020).…”

Section: Resultscontrasting

confidence: 70%

“…As saugeye production is highly variable due to limited sperm production per individual sauger compounded by fluctuations in populations and weather conditions, assisted production strategies are necessary for sustaining the multimillion dollar recreational fishery industry in Ohio reservoirs. Previous research has focused on managing sperm use (Blawut et al., 2018), long‐term storage of excess spermatozoa using cryopreservation (Blawut et al., 2020) and utilizing testicular harvest to enhance sperm yield (Blawut, Wolfe, Moraes, Sweet, et al., 2020). However, sperm quality (i.e., morphology, maturation and motility) can vary as a result of the unintended consequences of these assisted reproduction techniques and thus could adversely impact saugeye production.…”

Section: Introductionmentioning

confidence: 99%

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Spatial and Temporal Changes in Testis Morphology and Sperm Ultrastructure of the Sportfish Sauger (Sander canadensis)

et al. 2021

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The objective of this study was to assess testicular morphology and spermatozoal structure spatially within the reproductive tract and temporally among seasons in the sauger (Sander canadensis). The testis exists as two separate lobes joined at the urogenital pore and were characterised as unrestricted lobular with seminiferous tubules terminating at the ventral periphery and coalescing dorsally on the main sperm duct. Differences were observed between the pre‐breeding season (November) and breeding season (March), with every stage of spermatogenesis occurring in spermatocysts in pre‐breeding season in contrast to only spermatozoa being present in the tubules and main duct during the breeding season. Longitudinal folds in the main duct epithelium increased in number with increasing proximity to the urogenital pore, greatly increasing epithelial height regardless of season. Sauger spermatozoa consisted of an ovoid head, a midpiece containing 2 – 4 mitochondria incorporated into the head and a single flagellum containing an asymmetrical lateral ribbon. Motile spermatozoa were found throughout the testis during the breeding season. A decrease in sperm concentration was quantified moving proximally, suggesting a hydration effect by the main duct epithelium during the breeding season. These observations fill an important knowledge gap regarding reproductive biology of this impactful recreational fish species.

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Section: Resultscontrasting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Spatial and Temporal Changes in Testis Morphology and Sperm Ultrastructure of the Sportfish Sauger (Sander canadensis)

et al. 2021

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“…In the laboratory, the sperm concentration for each sample was determined by using an Equine 590b densimeter (Animal Reproductive Systems, Chino, California) that was previously validated for Sauger sperm in our laboratory (Blawut et al. ). A sample of milt was diluted with formalin in a cuvette, and absorbance was measured using a formalin‐zero blanked Equine 590b densimeter.…”

Section: Methodsmentioning

confidence: 99%

“…However, to date, there are few published reports on the basic reproductive characteristics in this species and no cryopreservation protocols have been established (Blawut et al. ).…”

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confidence: 99%

Use of Hypertonic Media to Cryopreserve Sauger Spermatozoa

et al. 2019

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The objective of this study was to determine the effects of extender osmolality on postthaw sperm quality and fertility in Sauger Sander canadensis. Fresh milt from 10 male Saugers was diluted by using base extenders with osmolalities of 350, 500, or 750 mOsm/kg (E350, E500, and E750, respectively) containing 10% dimethyl sulfoxide, frozen in LN 2 vapor, and stored. Sperm parameters (total motility, progressive motility, velocity, and viability) were assessed at different steps of the cryopreservation process (extended, equilibrated, and postthaw). Fertilization rates were compared between fresh and frozen sperm and at two sperm-to-egg ratios. All of the parameters that were measured, except for progressive motility, were reduced by cryopreservation. Extender 500 yielded the highest postthaw progressive motility (32.20 ± 3.86% [mean ± SD]) and velocity (84.97 ± 16.82 μm/s), whereas both E350 and E500 displayed the highest total motility (65.30 ± 4.24 and 68.70 ± 6.46%) and viability (80.60 ± 4.84 and 78.80 ± 3.91%), respectively. By contrast, E750 yielded the lowest postthaw velocity, viability, and total and progressive motility. Despite the increase in the motility parameters, fertilization in E350 (13.93%) was approximately double that in E500 (6.58%), although not statistically different. In conclusion, traditional isosmotic base extenders (E350) were found to be superior to hypertonic base extenders in the preservation of Sauger milt. These results serve as a starting point for future investigations of cryopreservation potential for Sauger spermatozoa that work toward developing a freezing protocol that is more suitable for large-scale application.

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“…The diversity and complexity of biological processes and reproductive strategies inherent to fishes have been increasingly studied over the past several years, leading to an expansion in interest and understanding of fish reproductive biology (Lowerre‐Barbieri et al ., 2011). Interest in reproductive biology has been motivated by everything from desires to understand basic biological functions (Poortenaar et al ., 2004) to enhanced information for population assessment and harvest (Midway & Scharf, 2012) to maximizing productivity in aquaculture settings (Blawut et al ., 2018). Recent advances in fish reproductive biology have moved towards holistic ways of considering suites of traits that govern reproductive strategies (Murua & Saborido‐Rey, 2003) and ultimately influence life history (Winemiller & Rose, 1992).…”

Section: Introductionmentioning

confidence: 99%

Reproductive biology of hardhead catfish Ariopsis felis: evidence for overwintering oocytes

Pensinger

Brown‐Peterson

Green

et al. 2021

Journal of Fish Biology

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Hardhead catfish Ariopsis felis are a common marine catfish in the coastal waters of the Gulf of Mexico (GOM). The low economic value of this species has depressed interest and research, and although the species is known for its extremely low fecundity and large oocytes, little else is known about this catfish species. A total of 1230 samples across all months of the year from 2016 to 2018 resulted in 681 females, and analysis of gonado-somatic index (I G ) revealed 1% to be a clear cut-off indicating maturity. Females are considered capable of spawning from April to June when I G averaged 4-8%. Both atresia and post-ovulatory follicles were present in July, suggesting that spawning ends in July in the northern GOM. The 1% I G cut-off was used to designate maturity, and from that an L 50 of 253 mm was estimated. Batch fecundity from 41 females estimated a mean batch size of 36 oocytes.Perhaps the most interesting finding was the presence of secondary growth stage oocytes (e.g., cortical alveoli) from July through November, well outside the spawning capable period. Furthermore, 78% of females had some early vitellogenic oocytes present during the non-spawning season, and the distribution of these relatively large (2-5 mm) oocytes did not change over time. The results here are not only important as reproductive biology information for a common and abundant species, but also present interesting and unusual patterns of non-spawning season oocyte development that is not commonly seen in Western Hemisphere subtropical fish species.

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Increasing saugeye (S. vitreus × S. canadensis) production efficiency in a hatchery setting using assisted reproduction technologies

Cited by 9 publications

References 24 publications

Spatial and Temporal Changes in Testis Morphology and Sperm Ultrastructure of the Sportfish Sauger (Sander canadensis)

Spatial and Temporal Changes in Testis Morphology and Sperm Ultrastructure of the Sportfish Sauger (Sander canadensis)

Use of Hypertonic Media to Cryopreserve Sauger Spermatozoa

Reproductive biology of hardhead catfish Ariopsis felis: evidence for overwintering oocytes

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Increasing saugeye (S. vitreus × S. canadensis) production efficiency in a hatchery setting using assisted reproduction technologies

Cited by 9 publications

References 24 publications

Spatial and Temporal Changes in Testis Morphology and Sperm Ultrastructure of the Sportfish Sauger (Sander canadensis)

Spatial and Temporal Changes in Testis Morphology and Sperm Ultrastructure of the Sportfish Sauger (Sander canadensis)

Use of Hypertonic Media to Cryopreserve Sauger Spermatozoa

Reproductive biology of hardhead catfish Ariopsis felis: evidence for overwintering oocytes

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Increasing saugeye (S. vitreus × S. canadensis) production efficiency in a hatchery setting using assisted reproduction technologies