Increasing Odontoblast-like Differentiation from Dental Pulp Stem Cells through Increase of β-Catenin/p-GSK-3β Expression by Low-Frequency Electromagnetic Field

Lim, Han-Moi; Nam, Myeong-Hyun; Kim, Yumi; Seo, Young-Kwon

doi:10.3390/biomedicines9081049

Cited by 9 publications

(7 citation statements)

References 68 publications

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“…Several studies have shown that YAP signaling plays an important role in osteogenesis and mechano-transduction. ,− Others have claimed that cytoskeletal reorganization activates β-catenin. For example, Sen et al found that dynamic strain induced actin restructuring, nuclear transport of G-actin, and nuclear access of β-catenin, and DPSCs on high rough electrospun polystyrene nanofibrous (PSF) matrix expressed higher levels of Wnt3a, nuclear β-catenin, and DSPP .…”

Section: Discussionmentioning

confidence: 99%

Topographic Cues of a PLGA Scaffold Promote Odontogenic Differentiation of Dental Pulp Stem Cells through the YAP/β-Catenin Signaling Axis

Yang

et al. 2023

ACS Biomater. Sci. Eng.

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Purpose: The underlying mechanism of how topographic cues of artificial scaffolds regulate cell function remains poorly understood. Yes-associated protein (YAP) and β-catenin signaling have both been reported to play important roles in mechano-transduction and dental pulp stem cells (DPSCs) differentiation. We investigated the effects of YAP and β-catenin in spontaneous odontogenic differentiation of DPSCs induced by topographic cues of a poly(lactic-co-glycolic acid) (PLGA) membrane. Methods: The topographic cues and function of a fabricated PLGA scaffold were explored via scanning electron microscopy (SEM), alizarin red staining (ARS), reverse transcription-polymerase chain reaction (RT-PCR), and pulp capping. Immunohistochemistry (IF), RT-PCR, and western blotting (WB) were used to observe the activation of YAP and β-catenin when DPSCs were cultured on the scaffolds. Further, YAP was inhibited or overexpressed on either side of the PLGA membrane, and YAP, β-catenin, and odontogenic marker expression were analyzed using IF, ARS, and WB. Results: The closed side of the PLGA scaffold promoted spontaneous odontogenic differentiation and nuclear translocation of YAP and β-catenin in vitro and in vivo compared to the open side. The YAP antagonist verteporfin inhibited β-catenin expression, nuclear translocation, and odontogenic differentiation on the closed side, but the effects were rescued by LiCl. YAP overexpressing DPSCs on the open side activated β-catenin signaling and promoted odontogenic differentiation. Conclusion: The topographic cue of our PLGA scaffold promotes odontogenic differentiation of DPSCs and pulp tissue through the YAP/β-catenin signaling axis.

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Section: Discussionmentioning

confidence: 99%

Topographic Cues of a PLGA Scaffold Promote Odontogenic Differentiation of Dental Pulp Stem Cells through the YAP/β-Catenin Signaling Axis

Yang

et al. 2023

ACS Biomater. Sci. Eng.

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“…To evaluate calcified minerals, the alizarin red was dissolved for 16 h with 5% 2-isopropanol (Sigma-Aldrich) and 10% acetic acid solution (Sigma-Aldrich). Absorbance was at 550 nm in a microplate reader spectrophotometer [ 15 , 16 ]. The calcium deposition was evaluated by performing a Von Kossa stain using a 5% silver nitrate (Sigma-Aldrich) solution under ultraviolet light for 20 min, followed by a 5% sodium thiosulphate (Sigma-Aldrich) solution for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Natural Bioactive Epigallocatechin-Gallate Promote Bond Strength and Differentiation of Odontoblast-like Cells

et al. 2023

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The (-)-Epigallocatechin-gallate (EGCG) metabolite is a natural polyphenol derived from green tea and is associated with antioxidant, biocompatible, and anti-inflammatory effects. Objective: To evaluate the effects of EGCG to promote the odontoblast-like cells differentiated from human dental pulp stem cells (hDPSCs); the antimicrobial effects on Escherichia coli, Streptococcus mutans, and Staphylococcus aureus; and improve the adhesion on enamel and dentin by shear bond strength (SBS) and the adhesive remnant index (ARI). Material and methods: hDSPCs were isolated from pulp tissue and immunologically characterized. EEGC dose-response viability was calculated by MTT assay. Odontoblast-like cells were differentiated from hDPSCs and tested for mineral deposition activity by alizarin red, Von Kossa, and collagen/vimentin staining. Antimicrobial assays were performed in the microdilution test. Demineralization of enamel and dentin in teeth was performed, and the adhesion was conducted by incorporating EGCG in an adhesive system and testing with SBS-ARI. The data were analyzed with normalized Shapiro–Wilks test and ANOVA post hoc Tukey test. Results: The hDPSCs were positive to CD105, CD90, and vimentin and negative to CD34. EGCG (3.12 µg/mL) accelerated the differentiation of odontoblast-like cells. Streptococcus mutans exhibited the highest susceptibility < Staphylococcus aureus < Escherichia coli. EGCG increased (p < 0.05) the dentin adhesion, and cohesive failure was the most frequent. Conclusion: (-)-Epigallocatechin-gallate is nontoxic, promotes differentiation into odontoblast-like cells, possesses an antibacterial effect, and increases dentin adhesion.

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“…These systems can typically contain different coil geometries that act as passive elements for magnetic field generation such as solenoids (Han et al, 2018;Sanie-Jahromi and Saadat, 2018;Costantini et al, 2019;Samiei et al, 2020;Mansoury et al, 2021;Salek et al, 2021;Maleki et al, 2022;Mansoury et al, 2022), circular (Yuan et al, 2018;López et al, 2019;García-minguillán et al, 2020;Xu et al, 2020;Yuan et al, 2020;Asadian et al, 2021) or square (Consales et al, 2018;Sun et al, 2018;Zuo et al, 2020;Elexpuru-Zabaleta et al, 2023;Lazzarini et al, 2023) coils arranged in different configurations, such as the Merrit configuration (Kirschvink, 1992) or the Helmholtz configuration (Kirschvink, 1992;López et al, 2019;García-minguillán et al, 2020;Xu et al, 2020;Asadian et al, 2021;Cios et al, 2021;Lim et al, 2021). The latter is one of the most popular configurations for exposing cell cultures or small animals to ELF-EMF (López et al, 2019;Garcíaminguillán et al, 2020;Xu et al, 2020;Asadian et al, 2021;Cios et al, 2021;Lim et al, 2021). In particular, the use of circular coils arranged in a Helmholtz configuration is one of the most widely used worldwide (Luo et al, 2016;Villarini et al, 2017;Ross et al, 2018;López et al, 2019;…”

Section: Introductionmentioning

confidence: 99%

“…The latter is one of the most popular configurations for exposing cell cultures or small animals to ELF-EMF (López et al, 2019;Garcíaminguillán et al, 2020;Xu et al, 2020;Asadian et al, 2021;Cios et al, 2021;Lim et al, 2021). In particular, the use of circular coils arranged in a Helmholtz configuration is one of the most widely used worldwide (Luo et al, 2016;Villarini et al, 2017;Ross et al, 2018;López et al, 2019;Yao et al, 2019;García-minguillán et al, 2020;Asadian et al, 2021;Cios et al, 2021;Lim et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Design and characterisation of a cell exposure system with high magnetic field homogeneity: RILZ coils

Rivera González,

López de Mingo,

Amuneke Ramírez

et al. 2024

Front. Bioeng. Biotechnol.

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In vitro studies requiring controlled exposure to low-frequency electromagnetic fields employ exposure systems with different geometries and configurations, the Helmholtz configuration being one of the most widely used. This configuration has limitations in the homogeneity of the spatial distribution of the magnetic field intensity values. We present the design, manufacturing, and characterisation of a new coil system, called RILZ configuration, which improves the distribution of magnetic field intensity values in the three dimensions of space for three different heights in comparison with the traditional circular coils in Helmholtz configuration. In addition, a comparative study of the cellular response in CT2A cultures exposed to a magnetic field of 50 Hz and 100 µT for 48 hrs is performed with both exposure systems. The results of the study show reduced values of deviation from the central value of magnetic field intensity using the RILZ coil system. These differences are statistically significant compared to the Helmholtz configuration for the three Cartesian directions: x (p < 0.01), y (p < 0.01), z (p < 0.01). In addition, the intensity values for three different heights are statistically significantly correlated using the RILZ coil system (p < 0.01). The differences in cell behaviour are also statistically significant between the two systems (p < 0.01) and may be directly related to the differences found in the distribution of intensity values between the two systems. This study highlights the importance of the homogeneity of the magnetic field intensity generated by the exposure systems used and offers an effective solution to control the magnetic field exposure parameters in vitro assays.

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Increasing Odontoblast-like Differentiation from Dental Pulp Stem Cells through Increase of β-Catenin/p-GSK-3β Expression by Low-Frequency Electromagnetic Field

Cited by 9 publications

References 68 publications

Topographic Cues of a PLGA Scaffold Promote Odontogenic Differentiation of Dental Pulp Stem Cells through the YAP/β-Catenin Signaling Axis

Topographic Cues of a PLGA Scaffold Promote Odontogenic Differentiation of Dental Pulp Stem Cells through the YAP/β-Catenin Signaling Axis

Natural Bioactive Epigallocatechin-Gallate Promote Bond Strength and Differentiation of Odontoblast-like Cells

Design and characterisation of a cell exposure system with high magnetic field homogeneity: RILZ coils

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