Increasing magnetite contents of polymeric magnetic particles dramatically improves labeling of neural stem cell transplant populations

Harnessing magnetic-mechano actuation in regenerative medicine and tissue engineering

“…Importantly, cell labelling with SPIONs appears not to affect viability, proliferation, or specification of MSCs into adipogenic, chondrogenic, or osteogenic lineages under in vivo conditions [72]. Similar findings were observed in magnetite-labelled neural stem cells [73].…”

“…These results, as well as the fact that uptake of SPIONs has no impact on viability or differentiation potential in a diverse array of cells [72,73], are good indicators of the safety of magnetically actuated materials. However, longterm in vivo tests must be undertaken before drawing further conclusions.…”

Part II: Functional delivery of a neurotherapeutic gene to neural stem cells using minicircle DNA and nanoparticles: Translational advantages for regenerative neurology

“…5C). Of the differentiated progeny, astrocytes were the predominant GFP + cell types (Fig.5D) as reported earlier (see manuscript part I) [49][50][51]. GFP expression was colocalised with extensive BDNF expression within cells (Fig.…”

Endocytotic Potential Governs Magnetic Particle Loading in Dividing Neural Cells: Studying Modes of Particle Inheritance

“…We recently proved that systematic tailored increases in the magnetite content of polymeric particles could significantly enhance cell labeling (>95% cells labeled) in the typically 'hard-to-label' transplant population of neural stem cells (NSCs) [8]. However, this study did not evaluate the longer-term retention of particles of different magnetite content by the labeled cells, or establish the pattern of 'inheritance' of particles by daughter NSCs post-proliferation.…”

“…20 h) making these ideal for capture of cell division events and dynamic imaging studies of particle inheritance [22,23]. Polymeric particles with different levels of magnetite content deployed in this study were formulated using biocompatible and biodegradable components highlighting their translational potential and justifying their use in this study [8]. The main study goals were to investigate the influence of tailored particle magnetite content on astrocyte loading and particle retention, while evaluating the safety of the methods and to investigate the profiles of particle inheritance in the daughter cells of labeled astrocytes using dynamic time-lapse imaging.…”