Increasing doses of phytase from Citrobacter braakii in diets with reduced inorganic phosphorus and calcium improve growth performance and lean meat of growing and finishing pigs

Abstract

The objective of this study was to evaluate the effect of increasing doses of bacterial phytase (RONOZYME HiPhos) on performance and carcass characteristics of growing and finishing pigs. The study included 120 castrated males with initial weight of 23.21 ± 1.91 kg and 68 days of age, distributed in a randomized block design with five treatments and eight replicates with three animals each. The pigs were fed five corn-soybean meal-based diets: positive control (PC), supplemented with inorganic phosphorus and calcium; negative control (NC), with 0.13% reduction in available phosphorus and 0.11% in calcium; and three NC diets supplemented with 1,000, 2,000, and 3,000 phytase units (FYT)/kg in the feed. Compared with the NC diets without phytase, diets with 1,000, 2,000, and 3,000 FYT/kg inclusion increased the daily weight gain by +12% (quadratic, p<0.05) during the growing I period; +2.9, +2.9, and +10.5% (linear, p<0.01), respectively, during the growing II period; and +4.1, +5.1, and +8.2% (linear, p<0.001), respectively, over the entire experimental period. The daily feed intake increased by 0, +2.8, and +4.3% (linear, p<0.05), respectively, considering the entire experimental period; and the final live weight increased by +3.2, +4.2, and +6.1% (linear, p<0.001), respectively. The phytase treatments did not influence feed conversion ratio, carcass weight and yield, backfat thickness, loin depth and carcass lean meat. According to the European Carcass Classification (SEUROP), however, the animals fed the PC diet and the three phytase levels had more carcasses classified as E (between 55–60% lean meat) when compared to carcasses of pigs fed the NC. Supplementing increasing levels of phytase to a corn- and soybean meal-based diet with inorganic P and Ca reduction improved daily weight gain and feed intake of growing pigs, and such effects were maintained until slaughter age.

A systematic-review on the role of exogenous enzymes on the productive performance at weaning, growing and finishing in pigs

“…Efficacy depends on various factors, such as pig growth stage, type of diet and source of Phytase ( Jongbloed, Van Diepen, Kemme & Broz, 2004 ). Increasing the level of enzyme inclusion does not necessarily represent a linear improvement in nutrient utilization ( Da Silva et al., 2019 ).…”

Improved sensitivity, accuracy and prediction provided by a high‐performance liquid chromatography screen for the isolation of phytase‐harbouring organisms from environmental samples

“…Adedokun et al ., 2015). Other bacterial AppA enzymes, e.g., from E. coli and Citrobacter spp., are used similarly (Sommerfeld et al ., 2018; da Silva et al ., 2019). Our unbiased, for phytase class, screening approach is clearly capable of identifying candidate phytases with potential as commercial leads.…”

“…The version described in this paper is version JABFFO010000000. Genomic completeness was analysed using BUSCO v3 (Simao et al, 2015), an opensource software that provides quantitative measures for genomic completeness based on evolutionarily informed expectations of gene content from near-universal singlecopy orthologs selected. The Acinetobacter sp.…”

Effects of supplementation of high‐dosing Trichoderma reesei phytase in the corn‐wheat‐soybean meal‐based diets on growth performance, nutrient digestibility, carcass traits, faecal gas emission, and meat quality in growing‐finishing pigs

“…The body weight and ADG were improved in growing‐finishing pigs receiving the diet supplemented with 1500 FTU/kg diet Trichoderma reesei phytase compared with those fed control diet. Previously, Guggenbuhl et al (2016), Dersjant‐Li et al (2017), da Silva et al (2019) reported that growing or finishing pigs fed a corn‐wheat‐soybean meal‐based or similar diet supplemented with high‐dosing (1000 to 3000 FTU/kg diet) microbial phytase had improved growth performance. However, these findings are inconsistent with the present study that the nutrients in the basal diet are insufficient.…”

“…With regard to the effect of dietary supplement with high‐dosing microbial phytase on carcass traits, some studies observed that the carcass weight and carcass back‐fat thickness of growing‐finishing pigs were not affected (Dersjant‐Li et al, 2017; Jang et al, 2017; Langbein et al, 2013; Malagutti et al, 2012; da Silva et al, 2019; Thacker et al, 2006). According to the report of Walz and Pallauf (2003), the carcass traits were not affected by any diet strategies.…”