Increasing DNA binding affinity of doxorubicin by loading on Fe3O4 nanoparticles: A multi-spectroscopic study

Mirzaei-Kalar, Zeinab; Yavari, A.; Jouyban, Abolghasem

doi:10.1016/j.saa.2019.117985

Cited by 21 publications

(10 citation statements)

References 32 publications

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“…Therefore, its continuous measurement in biological environments following treatment is crucial and required [ 11 , 12 , 13 , 14 , 15 ]. Numerous methods have been used for this purpose, such as high-performance liquid chromatography (HPLC), spectroscopic methods, and electrochemical sensors [ 11 , 16 , 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Doxorubicin Anticancer Drug Monitoring by ds-DNA-Based Electrochemical Biosensor in Clinical Samples

2021

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In this research, glassy carbon electrode (GCE) amplified with single-wall carbon nanotubes (SWCNTs) and ds-DNA was fabricated and utilized for voltammetric sensing of doxorubicin with a low detection limit. In this technique, the reduction in guanine signal of ds-DNA in the presence of doxorubicin (DOX) was chosen as an analytical factor. The molecular docking study revealed that the doxorubicin drug interacted with DNA through intercalation mode, which was in agreement with obtained experimental results. The DOX detection performance of ds-DNA/SWCNTs/GCE was assessed at a concentration range of 1.0 nM–20.0 µM. The detection limit was found to be 0.6 nM that was comparable and even better (in many cases) than that of previous electrochemical reported sensors. In the final step, the ds-DNA/SWCNTs/GCE showed powerful ability for determination of the DOX in injection samples with acceptable recovery data.

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Section: Introductionmentioning

confidence: 99%

Doxorubicin Anticancer Drug Monitoring by ds-DNA-Based Electrochemical Biosensor in Clinical Samples

2021

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“…[35][36][37][38] Loading of doxorubicin in nanoparticles increases its bioavailability and anti-tumor activity, DNA affinity and reduces its severe side-effects. [39][40][41] The idea of combined therapy by mixing more than two therapeutic agents in the form of a multifunctional nanoparticle platform has been introduced as a strategy to gain synergistic effects of therapeutic agents on disease treatment. 42,43 Hyperthermia or thermal therapy, ie raising the temperature of tumor tissues to 40-45°C, has provided acceptable benefits and is a promising approach for cancer treatment.…”

Section: Introductionmentioning

confidence: 99%

<p>Preparation and Evaluation of Doxorubicin-Loaded PLA–PEG–FA Copolymer Containing Superparamagnetic Iron Oxide Nanoparticles (SPIONs) for Cancer Treatment: Combination Therapy with Hyperthermia and Chemotherapy</p>

Khammarnia¹,

Nourbakhsh²,

Saber³

et al. 2020

IJN

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Background: Among the novel cancer treatment strategies, combination therapy is a cornerstone of cancer therapy. Materials and Methods: Here, combination therapy with targeted polymer, magnetic hyperthermia and chemotherapy was presented as an effective therapeutic technique. The DOX-loaded PLA-PEG-FA magnetic nanoparticles (nanocarrier) were prepared via a double emulsion method. The nanocarriers were characterized by particle size, zeta potential, morphology, saturation magnetizations and heat generation capacity, and the encapsulation efficiency, drug content and in-vitro drug release for various weight ratios of PLA:DOX. Then, cytotoxicity, cellular uptake and apoptosis level of nanocarrier-treated cells for HeLa and CT26 cells were investigated by MTT assay, flow cytometry, and apoptosis detection kit. Results and Conclusions: The synthesized nanoparticles were spherical in shape, had low aggregation and considerable magnetic properties. Meanwhile, the drug content and encapsulation efficiency of nanoparticles can be achieved by varying the weight ratios of PLA:DOX. The saturation magnetizations of nanocarriers in the maximum applied magnetic field were 59/447 emu/g and 28/224 emu/g, respectively. Heat generation capacity of MNPs and nanocarriers were evaluated in the external AC magnetic field by a hyperthermia device. The highest temperature, 44.2°C, was measured in the nanocarriers suspension at w/w ratio 10:1 (polymer:DOX weight ratio) after exposed to the magnetic field for 60 minutes. The encapsulation efficiency improved with increasing polymer concentration, since the highest DOX encapsulation efficiency was related to the nanocarriers' suspension at w/w ratio 50:1 (79.6 ± 6.4%). However, the highest DOX loading efficiency was measured in the nanocarriers' suspension at w/w ratio 10:1 (5.14 ± 0.6%). The uptake efficiency and apoptosis level of nanocarrier-treated cells were higher than those of nanocarriers (folic acid free) and free DOX-treated cells in both cell lines. Therefore, this targeted nanocarrier may offer a promising nanosystem for cancer-combined chemotherapy and hyperthermia.

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“…Regarding intercalation, the enhancement of DNA viscosity is associated with the DNA length, owing to the stacking of the planar ring of the molecule between DNA base pairs [20]. On the contrary, the electrostatic and groove-binding mode leads to a twisting or bending in the DNA helix, with a slight decrease in the viscosity [71,72]. A plot between relative specific viscosity (η/ηo) 1/3 versus the concentration of the complex 1/DNA ratio (r = 0.0, 0.05, 0.1, 0.15, 0.2, 0.25) is shown in Figure 6.…”

Section: Viscosity Measurementsmentioning

confidence: 99%

“…groove-binding mode leads to a twisting or bending in the DNA helix, with a slight decrease in the viscosity [71,72]. A plot between relative specific viscosity (η/ηo) 1/3 versus the concentration of the complex 1/DNA ratio (r = 0.0, 0.05, 0.1, 0.15, 0.2, 0.25) is shown in Figure 6.…”

Section: Viscosity Measurementsmentioning

confidence: 99%

Depicting the DNA Binding and Cytotoxicity Studies against Human Colorectal Cancer of Aquabis (1-Formyl-2-Naphtholato-k2O,O′) Copper(II): A Biophysical and Molecular Docking Perspective

Alolayqi¹,

Afzal²,

Alarifi³

et al. 2021

Crystals

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In this study, we attempted to examine the biological activity of the copper(II)–based small molecule aquabis (1-formyl-2-naphtholato-k2O,O′)copper(II) (1) against colon cancer. The characterization of complex 1 was established by analytical and spectral methods in accordance with the single-crystal X-ray results. A monomeric unit of complex 1 exists in an O4 (H2O) coordination environment with slightly distorted square pyramidal geometry (τ = ~0.1). The interaction of complex 1 with calf thymus DNA (ctDNA) was determined by employing various biophysical techniques, which revealed that complex 1 binds to ctDNA at the minor groove with a binding constant of 2.38 × 105 M–1. The cytotoxicity of complex 1 towards human colorectal cell line (HCT116) was evaluated by the MTT assay, which showed an IC50 value of 11.6 μM after treatment with complex 1 for 24 h. Furthermore, the apoptotic effect induced by complex 1 was validated by DNA fragmentation pattern, which clarified that apoptosis might be regulated through the mitochondrial-mediated production of reactive oxygen species (ROS) causing DNA damage pathway. Additionally, molecular docking was also carried out to confirm the recognition of complex 1 at the minor groove.

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Increasing DNA binding affinity of doxorubicin by loading on Fe3O4 nanoparticles: A multi-spectroscopic study

Cited by 21 publications

References 32 publications

Doxorubicin Anticancer Drug Monitoring by ds-DNA-Based Electrochemical Biosensor in Clinical Samples

Doxorubicin Anticancer Drug Monitoring by ds-DNA-Based Electrochemical Biosensor in Clinical Samples

<p>Preparation and Evaluation of Doxorubicin-Loaded PLA–PEG–FA Copolymer Containing Superparamagnetic Iron Oxide Nanoparticles (SPIONs) for Cancer Treatment: Combination Therapy with Hyperthermia and Chemotherapy</p>

Depicting the DNA Binding and Cytotoxicity Studies against Human Colorectal Cancer of Aquabis (1-Formyl-2-Naphtholato-k2O,O′) Copper(II): A Biophysical and Molecular Docking Perspective

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