Increasing detection of rotavirus G2P[4] strains in Nizhny Novgorod, Russia, between 2016 and 2019

Морозова, О. В.; Сашина, Т. А.; Епифанова, Н. В.; Kashnikov, Alexander Yu; Новикова, Н. А.

doi:10.1007/s00705-020-04853-7

Cited by 5 publications

(7 citation statements)

References 52 publications

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“…Analysis of the complete genotypes of double intergroup reassortants in 2017-2018 allowed us to establish their common clustering with DS-1-like G3P[8] rotaviruses isolated in other countries, for all 11 genes [31]. In the sample given in this study, which included reassortant G3P[8] RVAs isolated in 2020-2022, the nucleotide sequences of the VP6 gene were clustered with G2P [4] rotaviruses that were studied earlier [32].…”

Section: Discussionmentioning

confidence: 88%

“…The I-2 and I-3 lines include RVAs with genotypes G3P[6] and G3P [9] isolated in 1997-2016 and 1996-2014, respectively. In addition, cluster I includes a number of G3 rotaviruses isolated from pigs in Europe, Africa and Asia in 2009-2017 in combination with genotypes P[6], P [7], P [13], P [22], P [23], P [32] of the VP4 gene (in Figure 2 marked as Porcine G3).…”

Section: Resultsmentioning

confidence: 99%

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Phylogeographic characteristics of reassortant DS-1-like G3P[8]-strains of rotavirus type A isolated in Nizhny Novgorod (Russia).

Морозова

Сашина

Епифанова

et al. 2023

Preprint

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Since 2013, there has been an increase in reports of the spread of a double intergroup reassortant strain of rotavirus type A (RVA) with the genotype G3P[8] and other genes belonging to the second genogroup I2-R2-C2-M2-A2-N2-T2-E2-H2. Our study found an increase in the proportion of G3P[8] with a short migration profile of genome segments in RNA-PAGE from 15% (2020-2021) to 53% (2021-2022) in Nizhny Novgorod (Russia). The VP7, VP4 and VP6 genes were sequenced by the Sanger method. It was found that the studied strains belong to the genotype G3P[8]-I2, and are reassortants between Wa- and DS-1-like rotaviruses. Phylogenetic analysis revealed that VP7 sequences of studied strains are closely related to DS-1-like G3P[8] RVA, which have been isolated in other countries. The RVAs isolated in Nizhny Novgorod carried the allele P[8]-3.1 of the VP4 gene, as well as other worldwide spreading G3P[8] DS-1-like reassortants. None of the VP6 sequences of studied G3P[8] RVA were included in the group with equine-like strains isolated in other countries. Most VP6 alleles of Nizhny Novgorod G3P[8] DS-1-like RVAs clustered with G2P[4], which were studied earlier. This fact may indicate a local reassortment of the spread strains.

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Section: Discussionmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Phylogeographic characteristics of reassortant DS-1-like G3P[8]-strains of rotavirus type A isolated in Nizhny Novgorod (Russia).

Морозова

Сашина

Епифанова

et al. 2023

Preprint

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“…Earlier in Moscow, the proportion of this genotype fluctuated around 3% [10]. Most of the studied strains, together with strains from Russia, India, Japan, and Turkey, belonged to the G2-4a3 lineage that has spread worldwide since 2000 [24,25]. One strain belonged to the G2-4a1 lineage, together with strains from Russia, Italy, and the United States.…”

Section: Discussionmentioning

confidence: 93%

“…Viruses with the G9P [4] combination are widespread in Asia and South America, though sometimes they are detected in Europe [35]. In Russia, viruses with combinations of G8P [8] and G9P [4] were detected by PCR in Nizhny Novgorod, unfortunately they were not sequenced [25]. Viruses with G8P [8] and G9P [4] combinations belonged to DS-1-like strains [34,35].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Genetic Diversity of Group A Rotavirus Genotypes in Moscow (2019–2020)

et al. 2021

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Group A rotavirus (RVA) infection is the leading cause of hospitalization of children under 5 years old, presenting with symptoms of acute gastroenteritis. The aim of our study was to explore the genetic diversity of RVA among patients admitted to Moscow Infectious Disease Clinical Hospital No. 1 with symptoms of acute gastroenteritis. A total of 653 samples were collected from May 2019 through March 2020. Out of them, 135 (20.67%) fecal samples were found to be positive for rotavirus antigen by ELISA. RT-PCR detected rotavirus RNA in 80 samples. Seven G-genotypes (G1, G2, G3, G4, G8, G9, and G12) and three P-genotypes (P[8], P[4], and P[6]) formed 9 different combinations. The most common combination was G9P[8]. However, for the first time in Moscow, the combination G3P[8] took second place. Moreover, all detected viruses of this combination belonged to Equine-like G3P[8] viruses that had never been detected in Russia before. The genotype G8P[8] and G9P[4] rotaviruses were also detected in Moscow for the first time. Among the studied rotaviruses, there were equal proportions of Wa and DS-1-like strains; previous studies showed that Wa-like strains accounted for the largest proportion of rotaviruses in Russia.

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“…They serve for virus classification, with each categorized into a G-genotype (16 genotype) and P-genotype (27 genotype thus far) [98]. Despite the wide spectrum of rotavirus genotypes resulting from G/P combinations, epidemiological studies showed that the most prevalent genotypes are G1P(8), G3P(8), G4P(8), G9P(8), and G2P(4), which cause up to 90% of severe RVA infections worldwide [107,108]. Furthermore, there is no clear relationship between rotavirus genotypes and the severity of disease [109].…”

Section: Rotavirusmentioning

confidence: 99%

Insights into Gastrointestinal Virome: Etiology and Public Exposure

Nour

Hanif

Ryan

et al. 2021

Water

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Recycled wastewater is widely used owing to the potential shortage of water resources for drinking purposes, recreational activities, and irrigation. However, gut microbiomes of both human beings and animals negatively affect this water quality. Wastewater contamination is continuously monitored, using fecal contamination indicators or microbial source tracking approaches, to oppose arising enteric infections. Viral gastroenteritis is considered a principal manifestation of waterborne pathogenic virome-mediated infections, which are mainly transmitted via the fecal-oral route. Furthermore, acquired enteric viromes are the common cause of infantile acute diarrhea. Moreover, public exposure to wastewater via wastewater discharge or treated wastewater reuse has led to a significant surge of public health concerns. In this review, we discussed the etiology of waterborne enteric viromes, notably gastrointestinal virus infections, and public exposure to municipal wastewater. Conclusively, the early human virome is affected mainly by birth mode, dietary behavior, and maternal health, and could provide a signature of disease incidence, however, more virome diversification is acquired in adulthood. A multi-phase treatment approach offered an effective means for the elimination of wastewater reuse mediated public risks. The insights highlighted in this paper offer essential information for defining probable etiologies and assessing risks related to exposure to discharged or reused wastewater.

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Increasing detection of rotavirus G2P[4] strains in Nizhny Novgorod, Russia, between 2016 and 2019

Cited by 5 publications

References 52 publications

Phylogeographic characteristics of reassortant DS-1-like G3P[8]-strains of rotavirus type A isolated in Nizhny Novgorod (Russia).

Phylogeographic characteristics of reassortant DS-1-like G3P[8]-strains of rotavirus type A isolated in Nizhny Novgorod (Russia).

Prevalence and Genetic Diversity of Group A Rotavirus Genotypes in Moscow (2019–2020)

Insights into Gastrointestinal Virome: Etiology and Public Exposure

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