Increasing brightness in multiphoton microscopy with a low-repetition-rate, wavelength-tunable femtosecond fiber laser
Jakub Bogusławski,
Alicja Kwaśny,
Dorota Stachowiak
et al.
Abstract:Many experiments in biological and medical sciences currently use multiphoton microscopy as a core imaging technique. To date, solid-state lasers are most commonly used as excitation beam sources. However, the most demanding applications require precisely adjusted excitation laser parameters to enhance image quality. Still, the lag in developing easy-to-use laser sources with tunable output parameters makes it challenging. Here, we show that manipulating the temporal and spectral properties of the excitation b… Show more
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