Increases in peripheral SIRT1: A new biological characteristic of asthma

Abstract

Background and objective: Silent information regulator 1 (SIRT1) is a class III histone deacetylase that exerts both anti-inflammatory and anti-aging effects. However, no data are available regarding SIRT1 expression in patients with asthma. Here, we studied SIRT1 levels in the serum of patients with asthma and analysed the distribution of SIRT1 in both the serum and the lungs in an asthmatic mouse model to determine its clinical significance. Methods: Serum SIRT1 levels, total immunoglobulin E (IgE) levels and peripheral blood eosinophil percentages as well as pulmonary function were quantified in 97 patients with asthma and 118 healthy volunteers. BALB/c mice were sensitized and challenged using ovalbumin (OVA) to produce the asthmatic model, and SIRT1 levels in both the serum and the lung tissues were subsequently measured. Results: The serum SIRT1 levels were significantly elevated in the patients with asthma compared with the controls. Serum SIRT1 levels positively correlated with total IgE levels and negatively correlated with pulmonary function. In the OVA-sensitized and challenged mice, an increased serum SIRT1 level was confirmed, whereas decreased SIRT1 expression was observed in the lung tissues. Conclusions: These data indicate that lung SIRT1 expression decreased while serum SIRT1 increased in the setting of asthma. Serum SIRT1 levels correlate positively with both IgE levels and negatively with pulmonary function, suggesting that increased peripheral SIRT1 levels represent a new biological characteristic of asthma. Increased serum SIRT1 may be an auxiliary index for the diagnosis of asthma and elevating lung SIRT1 levels may be a new strategy for asthma therapy.

Year in review 2015: Asthma and chronic obstructive pulmonary disease

“…46 Serum SIRT1 levels were found to be significantly elevated in asthmatic patients, positively correlated with total IgE levels and negatively correlated with pulmonary function. 47 Also reported in Respirology, an increased serum SIRT1 level was confirmed in murine model of asthma, whereas decreased SIRT1 expression was observed in mouse lung tissue, similar to a previous study. 48 These findings suggest that high levels of serum SIRT1 are a biological characteristic of asthma and elevating lung SIRT1 levels with activators may be a new strategy for asthma therapy.…”

“…Sirtuin 1 (SIRT1), a member of the silent information regulator 2 family, belongs to class III histone deacetylase and is associated with airway inflammation and hyperresponsiveness in allergic asthma . Serum SIRT1 levels were found to be significantly elevated in asthmatic patients, positively correlated with total IgE levels and negatively correlated with pulmonary function . Also reported in Respirology , an increased serum SIRT1 level was confirmed in murine model of asthma, whereas decreased SIRT1 expression was observed in mouse lung tissue, similar to a previous study .…”

Suppression of Sirtuin-1 Increases IL-6 Expression by Activation of the Akt Pathway During Allergic Asthma

“…Moreover, they found that treatment of OVA mice with a SIRT1 activator decreased inflammatory cell lung infiltrates and IL-5 and IL-13 levels in the BAL fluid compared to those in the vehicle treatment [6] , which indicated the anti-inflammation effects of SIRT1. In addition, Wang et al also confirmed decreased SIRT1 expression in the lung tissues of OVA mice [7]. In asthma patients, Colley et al found SIRT1 activity was decreased in PBMCs from patients with severe asthma when compared to healthy subjects, and loss of SIRT1 activity increases IL-4 expression in patients with severe asthma [8].…”

The IL6R gene polymorphisms are associated with sIL-6R, IgE and lung function in Chinese patients with asthma

“…Spirometry was measured using standard spirometric techniques (POS Instrumentation, Inc., Louisville USA) according to the American Thoracic Society (ATS) guidelines as we previously described (Wang et al, 2015).…”