Increases in norepinephrine release and ovarian cyst formation during ageing in the rat

Acuña, Estefanía Flores; Fornes, Romina; Fernandois, Daniela; Garrido, Maritza P.; Greiner, Monika; Lara, Hernán E.; Paredes, Alfonso

doi:10.1186/1477-7827-7-64

Cited by 37 publications

(46 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Taking into account this limitation, further experiments are needed to confirm that each follicular cell express more KP with the aging. Beside this, we agreed, along with other authors, that both the noradrenaline content of the ovary and its release increase during aging (Chavez-Genaro et al 2007, Acuna et al 2009). Because we have also described the induction of KP by ISO (adrenergic agonist) in vitro (Ricu et al 2012), it is possible that the rise in adrenergic activity in the ovary that occurs naturally with aging provokes the increase in KP observed here.…”

Section: Ovarian Kp Increases During Aging and Is Correlated With Ovasupporting

confidence: 73%

“…By contrast, it has been described that with ovarian aging, women present a diminished follicular reserve (Faddy et al 1992, O'Connor et al 1998 and an increase in ovarian sympathetic nerves (Heider et al 2001). The rat model also presented an increase in ovarian adrenergic sympathetic tone with aging (Chavez-Genaro et al 2007, Acuna et al 2009, and this finding was correlated with an increase in the cystic structures and a decrease in ovulation (Acuna et al 2009), effects that were reversed under pharmacological b-adrenergic blockade (Fernandois et al 2012). Because we recently described through in vitro studies that KP levels increased under b-adrenergic stimulation (Ricu et al 2012), it is possible that the ovarian KP content also increases during ovarian aging.…”

Section: Introductionmentioning

confidence: 95%

“…The total amount of neurotransmitters released was calculated as the area under the curve after stimulation minus that of basal efflux (Ferruz et al 1991, Ricu et al 2008. The release percentages of 3H NE previously incorporated in 6-, 8-and 12-month-old rats were obtained from a previous publication by our laboratory (Acuna et al 2009). …”

Section: Uptake and Release Of Nementioning

confidence: 99%

“…The presence of secondary, antral, type III and cystic follicles was analyzed according to the work presented in Acuna et al (2009), Cruz et al (2012, Fernandois et al (2012) and Lara et al (2000). All of the slices were analyzed.…”

Section: Ovarian Morphologymentioning

confidence: 99%

“…The procedure was performed as previously described (Wakade 1980, Ricu et al 2008, Acuna et al 2009). The rats were sacrificed at 10 months of age.…”

Section: Uptake and Release Of Nementioning

confidence: 99%

See 4 more Smart Citations

Kisspeptin is involved in ovarian follicular development during aging in rats

Fernandois¹,

Na²,

Cuevas³

et al. 2015

Journal of Endocrinology

View full text Add to dashboard Cite

We have previously reported that kisspeptin (KP) may be under the control of the sympathetic innervation of the ovary. Considering that the sympathetic activity of the ovary increases with aging, it is possible that ovarian KP also increases during this period and participates in follicular development. To evaluate this possibility, we determined ovarian KP expression and its action on follicular development during reproductive aging in rats. We measured ovarian KP mRNA and protein levels in 6-, 8-, 10-and 12-month-old rats. To evaluate follicular developmental changes, intraovarian administration of KP or its antagonist, peptide 234 (P234), was performed using a mini-osmotic pump, and to evaluate FSH receptor (FSHR) changes in the senescent ovary, we stimulated cultured ovaries with KP, P234 and isoproterenol (ISO). Our results shows that KP expression in the ovary was increased in 10-and 12-month-old rats compared with 6-month-old rats, and this increase in KP was strongly correlated with the increase in ovarian norepinephrine observed with aging. The administration of KP produced an increase in corpora lutea and type III follicles in 6-and 10-month-old rats, which was reversed by P234 administration at 10 months. In addition, KP decreased the number and size of antral follicles in 6-and 10-month-old rats, while P234 administration produced an increase in these structures at the same ages. In ovarian cultures KP prevented the induction of FSHR by ISO. These results suggest that intraovarian KP negatively participates in the acquisition of FSHR, indicating a local role in the regulation of follicular development and ovulation during reproductive aging.

show abstract

Section: Ovarian Kp Increases During Aging and Is Correlated With Ovasupporting

confidence: 73%

Section: Introductionmentioning

confidence: 95%

Section: Uptake and Release Of Nementioning

confidence: 99%

Section: Ovarian Morphologymentioning

confidence: 99%

“…The procedure was performed as previously described (Wakade 1980, Ricu et al 2008, Acuna et al 2009). The rats were sacrificed at 10 months of age.…”

Section: Uptake and Release Of Nementioning

confidence: 99%

See 3 more Smart Citations

Kisspeptin is involved in ovarian follicular development during aging in rats

Fernandois¹,

Na²,

Cuevas³

et al. 2015

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

Analysis of different cell death processes of prepubertal rat oocytes in vitro

et al. 2010

View full text Add to dashboard Cite

The processes of cell death were studied in vitro in populations of oocytes isolated from prepubertal rats. In order to identify apoptosis, the externalized phosphatidylserine was recognized with Annexin-V coupled to FITC and the fragmentation of DNA was demonstrated by means of electrophoresis. Oocytes were tested for autophagy by means of the incorporation of monodansylcadaverine and monitoring Lc3-I/Lc3-II by western blot. The expression of mRNA marker genes of autophagy and of apoptosis was studied by means of RT-PCR in pure populations of oocytes. Some oocytes expressed at least one of the following markers: caspase-3, lamp1 and Lc3. Some oocytes were positive to Annexin-V or to monodansylcadaverine. However, most of them were simultaneously positive to both markers. The relative frequency of oocytes simultaneously positive to markers of apoptosis and autophagy did not change in the different ages studied. The transformation of Lc3-I in Lc3-II was present in all populations of oocytes studied. The mRNAs for caspase-3, lamp1 and Lc3 were present in all populations of oocytes analyzed. Our results demonstrate that oocytes of rats from new born to prepubertal age are eliminated by means of three different cell death processes: apoptosis, autophagy and a mixed event in which both routes to cell death participate in the same cell.

show abstract

Ovarian follicular dynamics after aromatizable or non aromatizable neonatal androgenization

Anesetti

Chávez-Genaro

2016

J Mol Hist

View full text Add to dashboard Cite

The effects of neonatal testosterone or dihydrotestosterone exposure on ovarian follicular dynamics were analysed at prepubertal, pubertal or adult age in Wistar rats. Both androgens induced a transitory increase on follicular endowment that was partially corrected at puberty. At adult age testosterone prevented ovulation, without significant modifications on follicular dynamics. An increased number of cystic structures were observed from puberty to adult age. However, ovaries of rats treated with dihydrotestosterone showed follicles with evident morphological alterations in granulosa and thecal layers although several corpora lutea were observed. A significant increase in preantral follicles and few cystic structures were detected at advanced adulthood. The size of cyst increased with age. No immunohistochemical changes on growth factors or enzymes related to steroidogenesis in growing follicles were obvious in any group. In both androgenized groups, cysts shared immunohistochemical characteristics exhibited by preovulatory follicles but they were unable to ovulate spontaneously. Our results provide an insight into the role of different androgens in female reproductive system development, indicating a direct effect of dihydrotestosterone on ovarian tissues whereas a central effect would be the main feature of neonatal testosterone exposure. Heterogeneous clinical manifestations seen in pathologies such as polycystic ovary syndrome among women could be associated with subtle hormonal changes during follicular population development.

show abstract

Increases in norepinephrine release and ovarian cyst formation during ageing in the rat

Cited by 37 publications

References 45 publications

Kisspeptin is involved in ovarian follicular development during aging in rats

Kisspeptin is involved in ovarian follicular development during aging in rats

Analysis of different cell death processes of prepubertal rat oocytes in vitro

Ovarian follicular dynamics after aromatizable or non aromatizable neonatal androgenization

Contact Info

Product

Resources

About