Increases in NMR-visible lipid and glycerophosphocholine during phenylbutyrate-induced apoptosis in human prostate cancer cells

Milkevitch, Matthew; Shim, Hyunsuk; Pilatus, Ulrich; Pickup, Stephen; Wehrle, Janna P.; Samid, Dvorit; Poptani, Harish; Glickson, Jerry D.; Delikatny, Edward J.

doi:10.1016/j.bbalip.2005.01.008

Cited by 83 publications

(102 citation statements)

References 38 publications

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“…The rise in GPC is consistent with our previous findings in human colon cancer cells and has previously been observed with the histone deacetylase (HDAC) inhibitor and differentiating agent phenylbutyrate 50 as well as the early PI3K inhibitor LY294002. 51 Whether this effect is related to the molecular events triggered post-HSP90 inhibition or to reduced proliferation and/or the induction of differentiation observed in our melanoma cells post-exposure to HSP90 inhibitors remains to be established.…”

Section: ©2 0 1 1 L a N D E S B I O S C I E N C E D O N O T D I S Tsupporting

confidence: 80%

Exploiting tumor metabolism for non-invasive imaging of the therapeutic activity of molecularly targeted anticancer agents

Beloueche‐Babari

Workman²,

Leach³

2011

Cell Cycle

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Section: ©2 0 1 1 L a N D E S B I O S C I E N C E D O N O T D I S Tsupporting

confidence: 80%

Exploiting tumor metabolism for non-invasive imaging of the therapeutic activity of molecularly targeted anticancer agents

Beloueche‐Babari

Workman²,

Leach³

2011

Cell Cycle

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“…To check whether the resonances from this metabolite are indicative of a cell death process, cells were treated with phenylbutyrate (PB), a chemotherapeutic agent that has been shown to induce apoptosis in certain malignant cells. 26,27 The 1 H NMR spectrum after incubation with PB (10 mM) for 24 h showed also a profile for low cell density and viability and, additionally, a significant accumulation of mobile lipids ( Figure 4c). In agreement with our expectation, a high increased of the signals assigned to acetyl-CoA was also observed (peaks at 2.24 and 0.67 ppm).…”

Section: Resultsmentioning

confidence: 99%

Detection of Metabolite Changes in C6 Glioma Cells Cultured with Antimitotic Oleyl Glycoside by ¹H MAS NMR

et al. 2009

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The synthetic glycoside, oleyl N-acetyl-R-D-glucosaminide (1), was previously shown to exhibit antimitotic activity on rat (C6) and human (U-373) glioma lines. To obtain information about its mechanism of action, metabolite changes in C6 glioma cells were analyzed after treatment with 1 using high-resolution magic angle spinning 1 H NMR. Compound 1 caused either a decrease or an increase in the intensity of the signal assigned to coenzyme A (CoA) metabolites depending on the concentration used. The data obtained from the 1 H NMR spectra of cells cultured with 1, combined with those obtained after treatment with oleic acid (an inhibitor of acetyl-CoA carboxylase) and phenyl butyrate (a known antineoplastic agent), suggest that 1 may be altering the metabolism of fatty acids and induce apoptosis of C6 glioma cells. These results point to NMR spectroscopy as an efficient technique for monitoring the response of the cells to therapeutic agents.

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“…In the case of phenylbutyrate, the rise in GPC levels was associated with mobile lipid accumulation and postulated to occur as a result of PLA2 activation (Milkevitch et al, 2005). The indomethacin-induced increase in cellular GPC was also attributed to enhanced membrane turnover through phospholipses (Glunde et al, 2006).…”

Section: Choline Phospholipid Metabolismmentioning

confidence: 91%

Metabolic assessment of the action of targeted cancer therapeutics using magnetic resonance spectroscopy

et al. 2009

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Developing rational targeted cancer drugs requires the implementation of pharmacodynamic (PD), preferably non-invasive, biomarkers to aid response assessment and patient follow-up. Magnetic resonance spectroscopy (MRS) allows the non-invasive study of tumour metabolism. We describe the MRS-detectable PD biomarkers resulting from the action of targeted therapeutics, and discuss their biological significance and future translation into clinical use.

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Increases in NMR-visible lipid and glycerophosphocholine during phenylbutyrate-induced apoptosis in human prostate cancer cells

Cited by 83 publications

References 38 publications

Exploiting tumor metabolism for non-invasive imaging of the therapeutic activity of molecularly targeted anticancer agents

Exploiting tumor metabolism for non-invasive imaging of the therapeutic activity of molecularly targeted anticancer agents

Detection of Metabolite Changes in C6 Glioma Cells Cultured with Antimitotic Oleyl Glycoside by ¹H MAS NMR

Metabolic assessment of the action of targeted cancer therapeutics using magnetic resonance spectroscopy

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Increases in NMR-visible lipid and glycerophosphocholine during phenylbutyrate-induced apoptosis in human prostate cancer cells

Cited by 83 publications

References 38 publications

Exploiting tumor metabolism for non-invasive imaging of the therapeutic activity of molecularly targeted anticancer agents

Exploiting tumor metabolism for non-invasive imaging of the therapeutic activity of molecularly targeted anticancer agents

Detection of Metabolite Changes in C6 Glioma Cells Cultured with Antimitotic Oleyl Glycoside by 1H MAS NMR

Metabolic assessment of the action of targeted cancer therapeutics using magnetic resonance spectroscopy

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Detection of Metabolite Changes in C6 Glioma Cells Cultured with Antimitotic Oleyl Glycoside by ¹H MAS NMR