Increases in HLA-DQ, DP, DR, and Transferrin Receptors on Alveolar Macrophages in Sarcoidosis and Allergic Alveolitis Compared with Fibrosing Alveolitis

Haslam, P L; Parker, D J; Townsend, P. J.

doi:10.1378/chest.97.3.651

Cited by 46 publications

(27 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have recently confirmed this finding, using techniques of flow cytonielric analysis, and have reported similar findings in patients with EAA [9]. We have also reported that levels of Ht-A-DQ and HLA-DP are signiiicanlly increased on alveolar macrophages in EAA and sarcoidosis compared with controls [9]. Alveolar macrophages are mainly derived from blood monoeytes, which migrate to the lungs where they complete their ditferentiation to mature alveolar macrophages [10,11]; and there is evidence that the infiltration of monocytes is increased in the lungs of patients with EAA [12].…”

Section: Introductionsupporting

confidence: 79%

“…Evidence in support of this suggestion was reported by Campbell et al [8], who observed that iilveoiar macrophages in BAL samples from patients with the granulomatous iung disease puimonary sarcoidosis show a significantly increased expression of HLA-DR compared with those from healthy controls. We have recently confirmed this finding, using techniques of flow cytonielric analysis, and have reported similar findings in patients with EAA [9]. We have also reported that levels of Ht-A-DQ and HLA-DP are signiiicanlly increased on alveolar macrophages in EAA and sarcoidosis compared with controls [9].…”

Section: Introductionsupporting

confidence: 71%

“…These limited responses occur in patients wilh a particular DQ haplotype, and removal of CD8 ^ T suppressor lymphocytes or the use of anti-DQ antibody augments the response in vitro. It is therefore of interest ihat in a study of a variety ofintcrstitial lung diseases, the highest median intensity of HLA-DQ expression by alveolar macrophages was observed in EAA [9], a disease reported to be associated with increased numbers of CD8^ T suppressor cells [3]. Furthermore, there is a close correlation between levels of lymphocytes, and levels of cholesterol and lipid-laden "foamy" macrophages in BAL samples from patients with EAA [14]; and there is evidence of an increased recruitmeni of monocytes to the lung [ 12].…”

Section: Discussionmentioning

confidence: 99%

“…The intensity of expression of HLA-D region products on monocytes was determined as we have previously reported [9]. The anti-log of the green (525 nm) Ruorescence emission was used to calculate the linear median intensity value for the monocytes stained wilh MoAb and for the controls without MoAb.…”

Section: Analysis Ofmrface Marker Expression By Flow Cytometrymentioning

confidence: 99%

See 3 more Smart Citations

Enhancement of the antigen-presenting function of monocytes by cholesterol: possible relevance to inflammatory mechanisms in extrinsic allergic alveolitis and atherosclerosis

Hughes¹,

Townsend²,

Haslam³

1992

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYExtrinsic allergic alveolitis (EAA) (synonym: hypcrsensilivity pneumonitis) is a hypersensitivity lung disease characterized by lymphocylic infiltrates in the pulmonary interstitial tissues. We have previously reported thai the numbers of lymphocytes in bronchoalveoiar iavage (BAL) samples in this disease correlate wilh leveis ofcholesierol and neutral lipid-ladcn 'foamy' macrophages. We have also reported that the macrophages express an increased density of MHC class II antigens (in particular HLA-DQ) which areknovtn lobe essential for antigen recognition by T lymphocytes. The aim of the present study was to explore whether cholesteroi is capable of enhancing the antigenpresenting function of mononuciear phagocytes by moduiating the expression of HLA-D region products. Ineubation of purified monocytes from heaithy volunteers with cholesterol in serum-free medium induced a significant increase in bolh ihe percentages of monocyles expressing HLA-DQ (P< 002) and in Ihe intensity of expression of the three HLA-D sub-region products, HLA-DQ,-DP and -DR {P<0Q2. <001, <005, respeetiveiy). The choieslerol pre-incubated monocytes also exhibited enhanced antigen-presenting function (/'<005), compared with controls pre-incubated without cholesterol. These findings indicate that increases in cholesterol in the extracellular milieu may augmeni aniigen presentation by modulating the expression of HLA-D region products on anligen-preseniing cells. Apart from EAA. this observation may also have relevance lo inflammatory mechanisms in atherosclerosis, where 'foamy' macrophages also occur in association with hypercholesterolaemia.

show abstract

Section: Introductionsupporting

confidence: 79%

Section: Introductionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Analysis Ofmrface Marker Expression By Flow Cytometrymentioning

confidence: 99%

See 2 more Smart Citations

Enhancement of the antigen-presenting function of monocytes by cholesterol: possible relevance to inflammatory mechanisms in extrinsic allergic alveolitis and atherosclerosis

Hughes¹,

Townsend²,

Haslam³

1992

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…If ACE induction in vivo is also restricted to the Leu-M2+ subpopulation of monocytes, it raises the interesting possibility that the presence of Leu-M2+ monocytes at the site of granuloma formation may be one of the factors that determine whether the resulting granuloma is ACE positive as in sarcoidosis, or ACE negative as in Crohn's disease. A recent report by Haslam et al [45] examining the distribution of HLA-DR, HLA-DQ and HLA-DP antigens on the macrophages recovered by bronchoalveolar lavage from sarcoidosis patients would support the concept that the HLA-DQ antigen may be important. These investigators found that, although the majority of alveolar macrophages from patients with sarcoidosis, fibrosing alveolitis and extrinsic allergic alveolitis all expressed HLA-DQ, DP and DR antigens, the density of HLA-DQ and DP was greater on macrophages from patients with the granulomatous diseases, sarcoidosis or extrinsic allergic alveolitis, than it was on macrophages from patients with nongranulomatous fibrosing alveolitis.…”

Section: Discussionmentioning

confidence: 91%

Monocyte heterogeneity in angiotensin-converting enzyme induction mediated by autologous T lymphocytes

Ryu

Vuk-Pavlović

Rohrbach

1992

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYThe ability of monocyte subpopulations to be induced selectively by T lymphocytes to synthesize enhanced levels of angiotensin-converting enzyme (ACE) was examined using an in vitro model etnploying normal peripheral blood monocytes and T lymphocytes. Separation of monocytes into subpopulations on the basis of buoyant density indicated no difference in the ability ofthe resulting monocyte subpopulations to produce basal levels of ACE when cultured in the absence of T lytnphocytes. However, the subpopulations differed significantly in their ability to synthesize enhanced levels of ACE in response to the presence of autologous T lymphocytes; low-density monocytes were induced by T lymphocytes to synthesize three-fold more ACE than were highdensity monocytes. Surface antigen labelling using MoAbs demonstrated that the low-density monocyte subpopulations also had a significantly higher percentage of Leu-M2"'" monocytes compared with the high-density monocyte subpopulations. When monocytes were separated on the basis of the presence of the Leu-M2 antigen using an immune rosetting technique, T lymphocytes were able to induce significantly elevated levels of ACE in the Leu-M2+ enriched monocyte subpopulation but were unable to induce ACE beyond basal levels in the Leu-M2+-depleted monocyte subpopulation. These results demonstrate that monocytes are heterogeneous with respect to their ability to be induced by T lymphocytes to synthesize ACE. This raises the possibility that selective aeeumulation of a monocyte subpopulation in the granulomatous inflammation of sarcoidosis may be one ofthe factors required for elevated ACE synthesis in the resulting granuloma epithelioid cells.

show abstract

Alveolar macrophage–T cell interactions during Th1‐type sarcoid inflammation

Agostini

Facco

Chilosi

et al. 2001

Microscopy Res & Technique

View full text Add to dashboard Cite

Sarcoidosis is an immunomediated, multisystem disorder of unknown cause(s) characterized by a heightened Th1 immune response that leads to an uncontrolled granuloma formation at sites of disease activity. The past few years have seen outstanding advances in the understanding of immunological and molecular events involved in the pathogenesis of this disease. The idea is that several cytokines and chemokines, which are secreted at sites of disease activity, participate in granuloma formation. This paper describes recent data that have clarified some of the events that govern the development of the hypersensitivity reaction during sarcoidosis. In particular, we will review recent evidence indicating that a complex relationship exists between the macrophage/lymphocyte cellular axis and the tissue networks of cytokines.

show abstract

Increases in HLA-DQ, DP, DR, and Transferrin Receptors on Alveolar Macrophages in Sarcoidosis and Allergic Alveolitis Compared with Fibrosing Alveolitis

Cited by 46 publications

References 28 publications

Enhancement of the antigen-presenting function of monocytes by cholesterol: possible relevance to inflammatory mechanisms in extrinsic allergic alveolitis and atherosclerosis

Enhancement of the antigen-presenting function of monocytes by cholesterol: possible relevance to inflammatory mechanisms in extrinsic allergic alveolitis and atherosclerosis

Monocyte heterogeneity in angiotensin-converting enzyme induction mediated by autologous T lymphocytes

Alveolar macrophage–T cell interactions during Th1‐type sarcoid inflammation

Contact Info

Product

Resources

About