Increased uterine NLRP3 inflammasome and leucocyte infiltration in a rat model of preeclampsia

Zeng, Huiqian; Han, Xinjia; Yu, Shengjun; Mei, Shanshan; Cheng, Xi; Zhang, Weiqiang; Zhang, Guanglan; Fang, Dajun

doi:10.1111/aji.13493

Cited by 7 publications

(4 citation statements)

References 51 publications

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“…There is a high level of NLRP3 expression in granulosa cells from patients with ovarian insufficiency, and the NLRP3 inflammasome has negative effects on female fertility ( 45 ). Activating the NLRP3 inflammasome disrupts the inflammatory microenvironment in the uterus, which leads to excessive inflammation at the maternal–fetal interface in rat preeclampsia ( 46 ). The NLRP3 inflammasome is activated in the myometrium tissues and amnion and chorion deciduas, which are associated with labor onset at term and preterm in humans and mice ( 47 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of early pregnancy on NOD-like receptor expression in the ovine endometrium

Zhang,

Cai,

Wang

et al. 2024

Front. Vet. Sci.

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IntroductionNucleotide-binding domain (NOD)-like receptors (NLRs) are expressed in the endometrium, and involved in modulating the female innate immune responses. There are conceptus-endometrial interactions during pregnancy, which ensure immune homeostasis of the maternal-fetal interface. The purpose of this study was to explore the effects of early pregnancy on NLR expression in the ovine endometrium.MethodsEndometrial tissues were collected at day 16 of the estrous cycle, and at days 13, 16 and 25 of pregnancy (n = 6 for each group), and RT-qPCR, western blot and immunohistochemistry analysis were used to analyze the expression of NLRs, including NOD1, NOD2, major histocompatibility complex class II transactivator (CIITA), neuronal apoptosis inhibitor protein (NAIP), NLR family, pyrin domain-containing 1 (NLRP1), NLRP3 and NLRP7.ResultsExpression levels of NOD1, NOD2, NAIP, CIITA, NLRP1 and NLRP3 declined, but expression level of NLRP7 increased in the endometria during early pregnancy compared with nonpregnant ewes. In addition, NOD2 and CIITA proteins were located in the endometrium in a protein type-, cell type- and pregnancy status-specific manner.DiscussionEarly pregnancy modulated expression of NLR family in the ovine endometrium, which may be essential for conceptus-endometrial interactions and maternal-fetal interface immune homeostasis.

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Section: Discussionmentioning

confidence: 99%

Effects of early pregnancy on NOD-like receptor expression in the ovine endometrium

Zhang,

Cai,

Wang

et al. 2024

Front. Vet. Sci.

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“…An accumulation of evidence now suggests that the NLRP3 inflammasome plays a pivotal role in the mechanism of inflammatory complications during pregnancy. Zeng et al found that the activation of NLRP3 inflammasome in the uterus is responsible for the excessive inflammation at the maternal–fetal interface during PE [ 44 ]. The NLRP3 inflammasome processes an increased “danger” of cytokines after the NLRP3 is activated.…”

Section: Discussionmentioning

confidence: 99%

NLRP3 inflammasome in peripheral blood monocytes as a risk factor for early -onset preeclampsia

Guo

et al. 2023

BMC Pregnancy Childbirth

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Introduction As a leading cause of pregnancy and fetal mortality, pre-eclampsia impacts about 5–8% of pregnancies globally. To date, few studies have focused on the role played by (NOD)-like receptors protein 3 (NLRP3) in peripheral blood in early-onset pre-eclampsia (PE). In this study, we investigated whether NLRP3 expression in monocytes before 20 weeks of gestation was associated with an increased risk of early-onset PE. Methodology During the study period from 2019 to 2021, women with singleton pregnancies were enrolled in this prospective study at the General Hospital of Northern Theater Command. A generalized additive model (GAM) and logistic regression models were applied to determine any association between NLRP3 and the risk of early-onset PE. Results In total, 571 and 48 subjects were included in the control and pre-eclampsia groups, respectively. The GAM and logistic regression models showed that NLRP3 was a significant factor for PE occurrence. The area under the curve, accuracy, specificity, sensitivity, positive likelihood ratio, negative likelihood ratio, and diagnostic odds ratio were 0.86, 0.82, 0.95, 0.72, 15.17, 0.29, and 52.0, respectively. Conclusion The monitoring for NLRP3 in peripheral blood may be a potential, prospectively identifying risk factor for preeclampsia.

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“…A variety of PreE animal models have been used to provide more insight into immunological mechanisms associated with PreE [38][39][40]. A study by Zeng et al which utilized a PreE rat model reported that PreE rats had higher mRNA expression of NLRP3 in uterine tissues compared to normal pregnant rats [41]. This group also found that both uterine protein expression of caspase-1 along with mRNA expression of caspase-1 and IL-1β were increased, leading them to conclude that the NLRP3 inflammasome has a key role in regulating uterine inflammation.…”

Section: Preclinical Studiesmentioning

confidence: 99%

Preeclampsia and COVID-19: the Role of Inflammasome Activation

et al. 2022

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Purpose of ReviewIt is well established that controlled immune activation and balance is critical for women's reproductive health and successful pregnancy outcomes. Research in recent decades in both clinical and animal studies has demonstrated that aberrant immune activation and inflammation play a role in the development and progression of women's reproductive health and pregnancy-related disorders. Inflammasomes are multi-protein cytoplasmic complexes that mediate immune activation. In this review, we summarize current knowledge on the role of inflammasome activation in pregnancy-related disorders.Recent Findings Increased activation of inflammasome is associated with multiple women's health reproductive disorders and pregnancy-associated disorders, including preeclampsia (PreE). Inflammasome activation is also associated with the novel coronavirus disease 2019 (COVID-19) disease caused by the SARS-Cov-2 virus. We and others have observed a positive association between increased PreE incidences with the onset of the COVID-19 pandemic. Here, we present our recent data indicating increased inflammasome activation, represented by caspase-1 activity, in women with COVID-19 and PreE compared to normotensive pregnant women COVID-19. Summary The role of inflammation in pregnancy-related disorders is an area of intense research interest. With the onset of the COVID-19 pandemic and the associated increase in PreE observed clinically, there is a greater need to identify mechanisms of pathophysiology and targets to treat this maternal disorder. Inflammasome activation is associated with PreE and COVID-19 infection and may hold therapeutic potential to improve outcomes associated with PreE and curb the morbidity attributed to PreE. Keywords Inflammasome • Preeclampsia • COVID-19 • NLRP3This article is part of the Topical Collection on Preeclampsia

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Increased uterine NLRP3 inflammasome and leucocyte infiltration in a rat model of preeclampsia

Cited by 7 publications

References 51 publications

Effects of early pregnancy on NOD-like receptor expression in the ovine endometrium

Effects of early pregnancy on NOD-like receptor expression in the ovine endometrium

NLRP3 inflammasome in peripheral blood monocytes as a risk factor for early -onset preeclampsia

Preeclampsia and COVID-19: the Role of Inflammasome Activation

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