Increased urothelial cancer associated 1 is associated with tumor proliferation and metastasis and predicts poor prognosis in colorectal cancer

Abstract

Long non-coding RNA, urothelial cancer associated 1 (UCA1), is reported to play a critical role in progression of carcinogenesis. In the present study, we identified differential expression of UCA1 in colorectal cancer (CRC) and paired peritumoral tissues using gene expression microarray analyses. qPCR analysis confirmed that UCA1 was upregulated in CRC (p<0.001) and the expression of UCA1 was statistically correlated with lymph node metastasis (P=0.040), distant metastasis (P=0.043) and tumor stage (P=0.010). Kaplan-Meier analysis indicated that patients with high UCA1 expression had a poor prognosis. Moreover, multivariate analysis identified UCA1 overexpression as an independent predictor for CRC. We also found that knockdown of UCA1 significantly suppressed cell proliferation and metastasis in CRC cells. Flow cytometry assays showed UCA1 silencing induced G0/G1 growth arrest and apoptosis of CRC cells. To further investigate the regulatory mechanisms of UCA1, we identified that Ets-2 bound to the UCA1 core promoter using luciferase assays. Collectively, our findings suggested that UCA1 might be an important prognostic indicator in CRC and may be a potential target for diagnosis and gene therapy.

LncRNA UCA1 promotes renal cell carcinoma proliferation through epigenetically repressing p21 expression and negatively regulating miR-495

“…21 Increased UCA1 was associated with tumor proliferation and metastasis and predicted poor prognosis in CRC. 22 In this study, we found that UCA1 was overexpressed in RCC tissues compared with the adjacent normal tissues, and higher expression levels of UCA1 were associated with advanced tumor stage.…”

“…21 Increased UCA1 was associated with tumor proliferation and metastasis and predicted poor prognosis in CRC. 22 In this study, we found that UCA1 was overexpressed in RCC tissues compared with the adjacent normal tissues, and higher expression levels of UCA1 were associated with advanced tumor stage.Numerous studies suggest that UCA1 play critical roles in some cancer development and progression. Wang et al 23 found that upregulation of UCA1 contributed to progression …”

Overexpression of long non-coding RNA-CTD903 inhibits colorectal cancer invasion and migration by repressing Wnt/β-catenin signaling and predicts favorable prognosis

“…Dozens of lncRNAs are reported to be potential biomarkers for cancer diagnosis and prognosis prediction in many solid tumors (8,9). Recently, LncRNA-H19, UCA1 and HOTAIR were also reported strongly correlated with CRC metastasis and prognosis (12)(13)(14). Therefore, lncRNAs are believed to represent a new category of cancer biomarkers and potential tumor therapeutic targets.…”

“…We previously identified aberrant expression of lncRNAs between CRC and matched paratumor normal tissues by a microarray analysis (13). Briefly, we selected six pairs of CRC tissues and corresponding paratumor normal tissues and assayed the expression of lncRNAs (30,586 items) and protein-coding mRNAs (26,109 items).…”

LncRNA UCA1 promotes the invasion and EMT of bladder cancer cells by regulating the miR‑143/HMGB1 pathway

“…UCA1 is highly expressed in diverse cancer types, such as breast cancer (17), pancreatic cancer (18), gastric cancer (19), and colorectal cancer (20,21), suggesting that high expression of UCA1 might serve as a molecular marker for predicting metastasis and prognosis in these cancers (22). Several studies also demonstrated that upregulation of UCA1 is associated with EMT in breast cancer (23) and bladder cancer (24), but the underlying mechanism remains largely unknown.…”