Increased transmigration of G–CSF‐mobilized peripheral blood CD34+ cells after overnight storage at 37°C

Greco, Nicholas J.; Lee, William R.; Moroff, Gary

doi:10.1046/j.1537-2995.2003.00572.x

Cited by 4 publications

(7 citation statements)

References 42 publications

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“…This stands in line with previous results from G-CSF mobilized, peripheral blood HPCs, whose chemotactic response increased after overnight storage at 37 1C. 21 We therefore analyzed the cellular receptor for SDF-1, that is the expression of CXCR-4 at different storage temperatures, and found that CXCR-4 expression was significantly higher in cord blood CD34 þ cells stored at RT than at 4 1C (approximately 75 versus 50%), and 15% higher than at baseline. This can only partly explain the differences in SDF-1 chemoattraction.…”

Section: Discussionsupporting

confidence: 93%

Optimum storage conditions for cord blood-derived hematopoietic progenitor cells prior to isolation

Moldenhauer

Wolf

Habermann

et al. 2007

Bone Marrow Transplant

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Optimum storage conditions of cord blood-derived hematopoietic progenitor cells before isolation remain unknown. We therefore evaluated CD34 þ cells isolated from cord blood units (n ¼ 57) within 1 h after collection and following storage for 24, 48 and 72 h at either room temperature (RT) or 4 1C. Isolated CD34 þ cells were analyzed for their cell count, immunophenotype, apoptosis rate, clonogenicity and transmigration capacity in response to stromaderived factor 1a using direct-paired comparisons (n ¼ 27). CD34 þ , CD133 þ and CD45 þ positivity after isolation remained the same under all conditions. After 24 h, CD34 þ cell counts and numbers of CFU-GM colonies dropped regardless of the storage temperature. After 48 h, the number of CD34 þ cells increased compared to 24 h, if the cord blood had been stored at RT resulting in almost three times more CD34 þ cells than at 4 1C. These cells had a lower early apoptosis rate and formed four times more BFU-E than those stored at 4 1C with equivalent plating efficiencies. CD34 þ cells kept at RT for 48 h had the highest transmigration capacities, which paralleled an increased CXCR-4 expression. Cord blood should be stored at RT before CD34 þ isolation and a storage time for 48 h should be preferred to 24 h.

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Section: Discussionsupporting

confidence: 93%

Optimum storage conditions for cord blood-derived hematopoietic progenitor cells prior to isolation

Moldenhauer

Wolf

Habermann

et al. 2007

Bone Marrow Transplant

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“…39 It is interesting we also noted a trend for elevated migration of rested cryopreserved cells toward the CXCR3 ligand CXCL11, despite unchanged CXCR3 expression (Fig. This is the closest in vitro assay to in vivo lymphocyte endothelial interactions and a good model of homing behavior.…”

Section: Effect Of Cryopreservation On Lymphocyte Homing Potentialmentioning

confidence: 67%

“…36 We detected an insignificant reduction in expression of L-selectin (CD62L) immediately after thawing as noted previously. 39 The mechanisms responsible for culture-induced increases in CXCR4 expression remain to be characterized but evidence from hematopoetic stem cells points to a role for culture conditions such as hypoxia, local cytokine release, 40 and interactions with other stromal cell compart-ments. 13,37 However, levels returned to normal on both CD4 and CD8 + lymphocytes after overnight culture ( 13 and this study).…”

Section: Effect Of Cryopreservation On Lymphocyte Homing Potentialmentioning

confidence: 99%

Functional Consequences of Human Lymphocyte Cryopreservation

Faint

Tuncer

Garg

et al. 2011

Journal of Immunotherapy

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In order to understand human inflammatory diseases and to develop and assess new therapeutic strategies targeting leukocyte recruitment to tissue, it is necessary to study human lymphocyte interactions with endothelium. It is often not practical to carry out assays on fresh human samples and therefore cells may be cryopreserved and batched for later study. Furthermore, many forms of adoptive cell therapy use cryopreserved cells that are required to migrate to tissue after infusion in vivo. The consequences of cryopreservation on the adhesion and migration of leukocytes is not known leading us to study the effects of cryopreservation on lymphocyte phenotype, migration, and adhesion. Cryopreservation and subsequent thawing did not alter the proportion of retrieved T cell subsets. Overall levels of expression of β1 or β2 integrins were unaffected but marked changes were observed in other relevant receptors. Expression of CD69, a transmembrane protein that plays a critical role in lymphocyte egress from tissues and the chemokine receptor CXCR4, increased on thawed populations and levels of CD62L and CXCR3 were reduced on thawed cells but restored if cells were allowed to recover after thawing. These changes were associated with modulation of the ability of lymphocytes to migrate across cytokine-stimulated monolayers of endothelium toward recombinant CXCL11 and CXCL12. Thus cryopreservation and thawing of lymphocytes induces changes in their adhesive phenotype and modulates their ability to migrate across endothelial monolayers. These findings have implications for in vitro experimentation and for cell therapy in which cryopreserved cells are expected to migrate when reinfused into patients.

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“…The impact of factors influencing the graft quality, such as liquid storage and transportation, have been investigated earlier, but the picture is complex . Storage, transport temperature, and duration are known to affect hematopoietic stem cell (CD34+) recovery over time.…”

mentioning

confidence: 99%

“…These methods do not detect cells in early apoptosis and may lead to overestimation of graft cell viability. Attempts have been made to find more refined methods that include detection of early apoptosis analysis . Today in many laboratories, including ours, there exist no robust methods to easily evaluate graft quality and how it impacts clinical outcome.…”

mentioning

confidence: 99%

Quality of the hematopoietic stem cell graft affects the clinical outcome of allogeneic stem cell transplantation

Watz¹,

Remberger²,

Ringdén³

et al. 2015

Transfusion

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BACKGROUND In approximately two‐thirds of patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) no suitable related donor can be identified but an unrelated HLA‐matched donor can be found through international donor registries. HSCT grafts from unrelated donors are commonly collected at distant sites. Therefore, graft storage and transportation becomes crucial in the HSCT process. We aimed to study the impact of graft quality on clinical outcome and identify factors affecting graft quality. STUDY DESIGN AND METHODS We investigated the influence of graft quality on the clinical outcome in 144 HSCT patients. Graft quality was assessed by determining the viability (7‐aminoactinomycin D [7AAD]) on a frozen‐thawed sample from the peripheral blood stem cell (PBSC) graft. RESULTS Patients receiving PBSCs with inferior quality (i.e., viability < 64% in the frozen‐thawed sample) more frequently developed acute graft‐versus‐host disease (aGVHD) Grades I to IV than patients receiving grafts with better quality (p = 0.025). The transplant‐related mortality (TRM) was higher in the group receiving grafts with lower viability (p = 0.03). The viability of the frozen‐thawed samples was highly variable (median, 64%; range, 24%‐96%). No correlation could be observed when comparing the viability in newly arrived PBSC grafts to frozen‐thawed vials. Grafts with white blood cell (WBC) count of more than 300 × 109/L had lower viability than those with lower WBC counts (p < 0.001). CONCLUSION Graft quality affects clinical outcome. Patients receiving grafts with inferior quality had more aGVHD and higher TRM. There is a need for better analyses for assessing graft quality in routine HSCT care; analysis using 7AAD on fresh PBSC grafts is not sufficient.

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Increased transmigration of G–CSF‐mobilized peripheral blood CD34+ cells after overnight storage at 37°C

Abstract: CD34+ cell transmigration was comparable between liquid-stored and cryopreserved samples, and both demonstrated similar increases after overnight incubation at 37 degrees C.

Cited by 4 publications

References 42 publications

Optimum storage conditions for cord blood-derived hematopoietic progenitor cells prior to isolation

Optimum storage conditions for cord blood-derived hematopoietic progenitor cells prior to isolation

Functional Consequences of Human Lymphocyte Cryopreservation

Quality of the hematopoietic stem cell graft affects the clinical outcome of allogeneic stem cell transplantation

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