2020
DOI: 10.1111/acel.13278
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Increased transcriptome variation and localised DNA methylation changes in oocytes from aged mice revealed by parallel single‐cell analysis

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Cited by 34 publications
(37 citation statements)
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“…A similar trend was observed with the hetero-dimer pair. Our results aligns with previous reports, where this bimodal distribution is a common feature reported in single-cell analysis of epigenetics, given the heterogeneous epigenetic content within a cell population ( Grosselin et al, 2019 , Castillo-Fernandez et al, 2020 ).…”
Section: Discussionsupporting
confidence: 93%
“…A similar trend was observed with the hetero-dimer pair. Our results aligns with previous reports, where this bimodal distribution is a common feature reported in single-cell analysis of epigenetics, given the heterogeneous epigenetic content within a cell population ( Grosselin et al, 2019 , Castillo-Fernandez et al, 2020 ).…”
Section: Discussionsupporting
confidence: 93%
“…This suggests that young oocytes are far more robust with regards to protein quality control than older oocytes. These findings are well supported by transcriptomic and single cell RNA sequencing studies (Grøndahl et al, 2010;Barragán et al, 2017;Wang et al, 2020) that demonstrate the dramatic modulation of mammalian oocyte transcriptomes with age, with the potential for these age-related effects on transcription to result in methylation differences (Castillo-Fernandez et al, 2020). These transcriptomic resources will enhance our understanding of changes in proteostasis that contribute to aging.…”
Section: The Contribution Of Declining Proteostasis To Oocyte Agingmentioning
confidence: 68%
“…In the human female germline, maternal methylation imprints are established during later stages of oocyte growth and for some genes may not be completed until shortly before pronuclear fusion [37]. However, consistent with the mouse model [13], the vast majority of human oocytes from both "younger" and "older" females displayed correct oocyte imprinting patterns. This also largely excludes somatic cell contamination and technical artefacts.…”
Section: Limitationsmentioning
confidence: 89%
“…In the mouse model, the majority of oocytes of older females displayed reduced complexity and increased variation of the transcriptome, associated with reduced developmental potential compared to oocytes from younger animals. Interestingly, a limited number of "old" oocytes exhibited a "young-like" epigenome [ 13 ]. Single oocyte methylomes from aged mice displayed decreased global CpG methylation (of single-copy genes), whereas our results show increased rDNA methylation in aging mouse and human oocytes.…”
Section: Discussionmentioning
confidence: 99%
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