Increased tolerance to two oomycete pathogens in transgenic tobacco expressing pathogenesis-related protein 1a.

Alexander, Danny; Goodman, Robert M.; Gut-Rella, Manuela; Glascock, Christopher; Weymann, Kris; Friedrich, Leslie; Maddox, Daryl W.; Ahl-Goy, Patricia; Luntz, Thomas L.; Ward, Eric

doi:10.1073/pnas.90.15.7327

Cited by 491 publications

(250 citation statements)

References 32 publications

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“…Interestingly, the ECDs in two novel rice receptors, PR-1/SCP and chitinase RLKs, are both pathogenesis-related proteins implicated in defense responses. In tobacco (Nicotiana tabacum), the expression of PR-1a is tightly correlated with the onset of systemic acquired resistance, and its overexpression results in higher tolerance to fungal pathogens (Alexander et al, 1993). Our findings indicate that new receptors were made and retained through the process of selection in these two plant lineages independently.…”

Section: Evolution Of Domains In Rlksmentioning

confidence: 71%

Comparative Analysis of the Receptor-Like Kinase Family in Arabidopsis and Rice[W]

et al. 2004

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Receptor-like kinases (RLKs) belong to the large RLK/Pelle gene family, and it is known that the Arabidopsis thaliana genome contains >600 such members, which play important roles in plant growth, development, and defense responses. Surprisingly, we found that rice (Oryza sativa) has nearly twice as many RLK/Pelle members as Arabidopsis does, and it is not simply a consequence of a larger predicted gene number in rice. From the inferred phylogeny of all Arabidopsis and rice RLK/Pelle members, we estimated that the common ancestor of Arabidopsis and rice had >440 RLK/Pelles and that largescale expansions of certain RLK/Pelle members and fusions of novel domains have occurred in both the Arabidopsis and rice lineages since their divergence. In addition, the extracellular domains have higher nonsynonymous substitution rates than the intracellular domains, consistent with the role of extracellular domains in sensing diverse signals. The lineagespecific expansions in Arabidopsis can be attributed to both tandem and large-scale duplications, whereas tandem duplication seems to be the major mechanism for recent expansions in rice. Interestingly, although the RLKs that are involved in development seem to have rarely been duplicated after the Arabidopsis-rice split, those that are involved in defense/disease resistance apparently have undergone many duplication events. These findings led us to hypothesize that most of the recent expansions of the RLK/Pelle family have involved defense/resistance-related genes.

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Section: Evolution Of Domains In Rlksmentioning

confidence: 71%

Comparative Analysis of the Receptor-Like Kinase Family in Arabidopsis and Rice[W]

et al. 2004

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“…Overall, two facts have emerged from these overexpression experiments. First, the basic forms of PR proteins, which are targeted to the vacuole, exhibit more effective antifungal activity than their acidic counterparts, which are secreted from the plant cell (Sela-Buurlage et al, 1993); the only exception to date is the tobacco PR-la protein (Alexander et al, 1993). Second, when two or more PR proteins are constitutively coexpressed, a synergistic increase in the leve1 of disease control can be obtained (Zhu et al, 1994).…”

Section: Pr Proteins and Other Defense-related Proteinsmentioning

confidence: 99%

Resistance gene-dependent plant defense responses.

1996

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“…On the other hand, several crops which exhibit tolerance to fungal pathogens have been developed among the higher terrestrial plants by using the transgenic technique (2,9,33,35,38,40,59). Many of the resistance genes used for transformation of these plants encode polysaccharolytic enzymes, such as chitinase (8,9,23,51) and ␤-1,3-glucanase (7,23,45,51,60), which should degrade the cell walls of invading fungi.…”

mentioning

confidence: 99%

An Antifungal Protein from the Marine Bacterium Streptomyces sp. Strain AP77 Is Specific for Pythium porphyrae , a Causative Agent of Red Rot Disease in Porphyra spp

Woo

Kitamura

Myouga

et al. 2002

Appl Environ Microbiol

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A novel antifungal protein (SAP) was found in the culture supernatant of a marine bacterium, Streptomyces sp. strain AP77, and was purified. This protein was characterized by chemical, biochemical, and biological analyses. By using gel filtration, the molecular mass of SAP was estimated to be 160 kDa. Structural analysis of SAP by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-time of flight mass spectrometry suggested that SAP is composed of three heterologous protein subunits of 41.7 kDa (SAP1), 21.7 kDa (SAP2), and 18.7 kDa (SAP3) at a molar ratio of 1:1:5 (or 1:1:6). N-terminal amino acid sequence analysis and a homology search revealed that SAP1, SAP2, and SAP3 exhibit 64.3, 68.4, and 86.7% similarity to three Streptomyces coelicolor polypeptides, puromycin resistance protein (Pur8), a conserved hypothetical protein, and bacterioferritin, respectively. The MIC of purified SAP against Pythium porphyrae was determined to be 1.6 g/disk, whereas no inhibitory effect was observed at concentrations up to 100 g/disk against most of the fungal and bacterial strains tested; the only exception was relatively strong antifungal activity against Pythium ultimum (MIC, 6.3 g/disk). In vitro and in vivo toxicity tests demonstrated that SAP showed no toxicity against Porphyra yezoensis cells, human normal dermal fibroblasts, and mice at doses up to 700 g/ml (for 24 h), 250 g/ml (for 12 h), and 75 mg/kg (for 35 days), respectively. SAP was labile when it was subjected to a heated-air drying treatment, which is a great advantage in food production procedures. These results indicated that Streptomyces sp. strain AP77 might be useful as a gene source for safe transgenic Porphyra breeding for tolerance to Pythium infection.

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Increased tolerance to two oomycete pathogens in transgenic tobacco expressing pathogenesis-related protein 1a.

Cited by 491 publications

References 32 publications

Comparative Analysis of the Receptor-Like Kinase Family in Arabidopsis and Rice[W]

Comparative Analysis of the Receptor-Like Kinase Family in Arabidopsis and Rice[W]

Resistance gene-dependent plant defense responses.

An Antifungal Protein from the Marine Bacterium Streptomyces sp. Strain AP77 Is Specific for Pythium porphyrae , a Causative Agent of Red Rot Disease in Porphyra spp

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