Increased tolerance to salt stress in the phosphate-accumulating Arabidopsis mutants siz1 and pho2

Miura, Kenji; Sato, Aiko; Ohta, Masaru; Furukawa, Yoshihiro

doi:10.1007/s00425-011-1476-y

Cited by 60 publications

(54 citation statements)

References 77 publications

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“…This group includes the SUMO E3 ligase SIZ1 (Miura et al, 2011), the phospholipases PLDz1 and PLDz2 ( Fig. 6A; Li et al, 2006;Miura et al, 2011), the phosphatidylinositol-4-phosphate 5-kinase At1g60890 (Stenzel et al, 2008;Wada et al, 2015), the phosphatidylinositol-4-phosphate phosphatase RHD4 (Stenzel et al, 2008;Thole et al, 2008;Wada et al, 2015), the ubiquitinspecific protease UBP14 (Li et al, 2010), the patterning protein TRN1 (Kwak et al, 2015), and the P5-type ATPase PDR2, a key player in root Pi sensing (Wang et al, 2010;Müller et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Deubiquitinating Enzyme OTU5 Contributes to DNA Methylation Patterns and Is Critical for Phosphate Nutrition Signals

et al. 2017

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ORCID IDs: 0000-0002-4868-8284 (D.-F.S.); 0000-0002-7850-6832 (W.S.); 0000-0002-7402-3075 (P.-Y.C.).Phosphate (Pi) starvation induces a suite of adaptive responses aimed at recalibrating cellular Pi homeostasis. Plants harboring a mutation in OVARIAN TUMOR DOMAIN-CONTAINING DEUBIQUITINATING ENZYME5 (OTU5) showed altered DNA methylation of root hair-related genes and altered Pi-responsive root traits. Unlike the wild type, homozygous otu5 mutants did not respond to Pi starvation by increased lateral root formation and increased root hair length but formed very short root hairs when grown on low-Pi media. Under Pi-replete conditions, otu5 plants developed more root hairs than the wild type due to attenuated primary root growth, a phenotype that resembled that of Pi-deficient plants. Growth of plants on low-Pi media altered both H3K4 and H3K27 trimethylation levels at the transcriptional start site of a subset of genes encoding key players in Pi homeostasis, which was correlated with mRNA abundance changes of these genes. Pi starvation had a minor impact on DNA methylation. Differentially methylated regions were enriched in transposable elements, suggesting that DNA methylation associated with low Pi supply is required for maintaining genome integrity. It is concluded that DNA methylation and histone methylation constitute critical, interdependent regulatory components that orchestrate the activity of a subset of Pi-responsive genes.

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Section: Discussionmentioning

confidence: 99%

Deubiquitinating Enzyme OTU5 Contributes to DNA Methylation Patterns and Is Critical for Phosphate Nutrition Signals

et al. 2017

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“…PCR products were detected by ermal Cycler Dice Real Time System (Takara Bio Inc., Kyoto, Japan). Relative di erences in expression were calculated as described previously (Miura et al 2011b;Miura et al 2011c). Brie y, relative transcript abundance was calculated using the comparative C T method (User Bulletin 2 for ABI PRISM 7700 sequence detection systems).…”

Section: Rna Isolation and Quantitative Rt-pcr Analysismentioning

confidence: 99%

SlICE1 encoding a MYC-type transcription factor controls cold tolerance in tomato, Solanum lycopersicum

Miura¹,

Shiba²,

Ohta³

et al. 2012

Plant Biotechnology

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Many abiotic and biotic stresses can reduce plant growth and development. Low temperature is one of the most harmful abiotic stresses, particularly for plants that are tropical or subtropical in origin. To elucidate the molecular mechanisms underlying the cold-stress response, components involved in the signal transduction of cold stress have been characterized. In this study, we characterized a basic helix-loop-helix (bHLH) transcription factor encoding gene, SlICE1, from tomato (Solanum lycopersicum), which shows similarity with Arabidopsis ICE1. e expression of SlICE1 was observed in younger leaves, owers, and green and red fruits. To characterize the function of SlICE1, overexpressing tomato lines were produced. SlICE1-overexpressing tomatoes exhibited chilling tolerance, and SlICE1 enhanced the expression of coldresponsive genes, such as SlCBF1 and SlDRCi7, as well as accumulation of ascorbic acid. e SlICE1 protein was degraded a er cold treatment. ese results indicate that SlICE1 enhances cold tolerance in tomatoes.

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“…Mutants of the Arabidopsis E3 ligase SIZ1 show increased sensitivity to phosphate deprivation, excess copper, temperature stress, and drought stress (Miura et al, 2005(Miura et al, , 2007Yoo et al, 2006;Catala et al, 2007;Chen et al, 2011). Interestingly, siz1 (E3 ligase) mutants in Arabidopsis show increased tolerance to salt stress (Miura et al, 2011), whereas the SUMO protease (isopeptidase) double mutant, ots1 ots2 ( for overly sensitive to salt1 overly sensitive to salt2) shows extreme sensitivity to salt (Conti et al, 2008). Another indirect indication of the role of SUMOylation in the response of plants to stress is the recent observation linking changing levels of SUMOylation to changing levels of salicylic acid (Villajuana-Bonequi et al, 2014).…”

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confidence: 99%

SUMOylation by a Stress-Specific Small Ubiquitin-Like Modifier E2 Conjugase Is Essential for Survival ofChlamydomonas reinhardtiiunder Stress Conditions

Knobbe¹,

Horken²,

Plucinak³

et al. 2015

Plant Physiology

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Posttranslational modification of proteins by small ubiquitin-like modifier (SUMO) is required for survival of virtually all eukaryotic organisms. Attachment of SUMO to target proteins is catalyzed by SUMO E2 conjugase. All haploid or diploid eukaryotes studied to date possess a single indispensable SUMO conjugase. We report here the unanticipated isolation of a Chlamydomonas reinhardtii (mutant5 [mut5]). in which the previously identified SUMO conjugase gene C. reinhardtii ubiquitinconjugating enzyme9 (CrUBC9) is deleted. This surprising mutant is viable and unexpectedly, displays a pattern of protein SUMOylation at 258C that is essentially identical to wild-type cells. However, unlike wild-type cells, mut5 fails to SUMOylate a large set of proteins in response to multiple stress conditions, a failure that results in a markedly reduced tolerance or complete lack of tolerance to these stresses. Restoration of expected stress-induced protein SUMOylation patterns as well as normal stress tolerance phenotypes in mut5 cells complemented with a CrUBC9 gene shows that CrUBC9 is an authentic SUMO conjugase and, more importantly, that SUMOylation is essential for cell survival under stress conditions. The presence of bona fide SUMOylated proteins in the mut5 mutant at 258C can only be explained by the presence of at least one additional SUMO conjugase in C. reinhardtii, a conjugase tentatively identified as CrUBC3. Together, these results suggest that, unlike all other nonpolyploid eukaryotes, there are at least two distinct and functional SUMO E2 conjugases in C. reinhardtii, with a clear division of labor between the two sets: One (CrUBC9) is involved in essential stress-induced SUMOylations, and one (CrUBC3) is involved in housekeeping SUMOylations.

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Increased tolerance to salt stress in the phosphate-accumulating Arabidopsis mutants siz1 and pho2

Cited by 60 publications

References 77 publications

Deubiquitinating Enzyme OTU5 Contributes to DNA Methylation Patterns and Is Critical for Phosphate Nutrition Signals

Deubiquitinating Enzyme OTU5 Contributes to DNA Methylation Patterns and Is Critical for Phosphate Nutrition Signals

SlICE1 encoding a MYC-type transcription factor controls cold tolerance in tomato, Solanum lycopersicum

SUMOylation by a Stress-Specific Small Ubiquitin-Like Modifier E2 Conjugase Is Essential for Survival ofChlamydomonas reinhardtiiunder Stress Conditions

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