Increased telomerase activity and human telomerase reverse transcriptase mRNA expression in the endometrium of patients with endometriosis

Kim, C. M.; Oh, Yang-Hyo; Cho, S. H.; Chung, Dawn; Hwang, Jeongjae; Park, K. H.; Cho, D. J.; Choi, Youngmin; Lee, Byung Seok

doi:10.1093/humrep/del425

Cited by 39 publications

(39 citation statements)

References 27 publications

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“…Several studies have demonstrated that both telomerase activity and expression are significantly upregulated in the endometrium with endometriosis, which also shows subfertility and low implantation rate (Kim et al, 2007;Hapangama et al, 2008b;Mafra et al, 2014). Considering our data, we concluded that telomerase may contribute to a dysfunctional endometrium during the implantation window in RIF patients.…”

Section: Discussionsupporting

confidence: 59%

“…Telomerase has been well studied in the context of cell oncogenesis, senescence, and immortalization (Stewart et al, 2012;Ye et al, 2014). Recently, several studies have also revealed that telomerase is closely involved in female reproductive health (Kim et al, 2007;Hapangama et al, 2008a;Hapangama et al, 2008b;Chen et al, 2011;Mafra et al, 2014). In the human endometrium, the mRNA of telomerase reverse transcriptase (Tert), which encodes the catalytic subunit of telomerase, is highly expressed in epithelial cells (Tanaka et al, 1998;Hapangama et al, 2008b).…”

Section: Introductionmentioning

confidence: 99%

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Endometrial expression of telomerase, progesterone, and estrogen receptors during the implantation window in patients with recurrent implantation failure

Long

Liu

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Telomerase plays a critical role in cell proliferation and senescence, but the exact involvement of endometrial telomerase in recurrent implantation failure (RIF) is unknown. We collected endometrial biopsies from RIF patients (N = 30) and fertile women (N = 30). Real-time PCR was performed for detecting changes in telomerase reverse transcriptase (Tert), ER alpha, and PR expression at the transcript level, and the correlation between the variable expressions of these genes was tested using regression analysis. Then, western blot and immunohistochemistry were used to analyze the expression profiles of TERT and ER alpha at the protein level. Compared to the control, Tert expression was substantially increased, whereas ER alpha expression significantly decreased in the endometrium with RIF. No change was observed in PR expression. Tert expression was inversely associated with ER alpha expression. TERT protein expression in RIF patients was also clearly elevated, and was localized to both the endometrial epithelium and stromal cells. However, the signals for ER alpha in the stromal cells were weaker than those in the control. Expression of endometrial telomerase was substantially enhanced as ER alpha decreased in RIF patients during the implantation window.

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Section: Discussionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Endometrial expression of telomerase, progesterone, and estrogen receptors during the implantation window in patients with recurrent implantation failure

Long

Liu

et al. 2016

Genet. Mol. Res.

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“…Irregular function of these SCs may contribute to the pathogenesis of endometriosis and the growth of tissue outside the uterine cavity, causing dysmenorrhoea, subfertility and endometrial carcinoma [30]. Stem cells that are ectopically distributed through lymphovascular metastasis [31] may also contribute to the pathogenetic process, because their high proliferation promotes rapid clonal expansion [32]. …”

Section: Introductionmentioning

confidence: 99%

Endometrial stem cells in regenerative medicine

et al. 2014

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First described in 2004, endometrial stem cells (EnSCs) are adult stem cells isolated from the endometrial tissue. EnSCs comprise of a population of epithelial stem cells, mesenchymal stem cells, and side population stem cells. When secreted in the menstrual blood, they are termed menstrual stem cells or endometrial regenerative cells. Mounting evidence suggests that EnSCs can be utilized in regenerative medicine. EnSCs can be used as immuno-modulatory agents to attenuate inflammation, are implicated in angiogenesis and vascularization during tissue regeneration, and can also be reprogrammed into induced pluripotent stem cells. Furthermore, EnSCs can be used in tissue engineering applications and there are several clinical trials currently in place to ascertain the therapeutic potential of EnSCs. This review highlights the progress made in EnSC research, describing their mesodermal, ectodermal, and endodermal potentials both in vitro and in vivo.

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“…The abnormal existence or hyperplasia of cells is thought to be associated with the pathogenesis of a number of diseases (8). Numerous putative stem cell markers have been previously described (9,10). Differentiated cells are produced by the ectopic expression of specific transcription factors, including SRY-box containing gene 2 (SOX2), octamer-binding transcription factor 4 (Oct-4), Krüeppel-like factor 4 (KLF-4), homeobox protein NANOG (NANOG), C-X-C chemokine receptor type 4 (CXCR4) and cellular homolog of the oncogenic retrovirus v-myc oncogene (c-Myc), and subsequently form pluripotent stem cells (11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach

Hwang

Wang

et al. 2013

Molecular Medicine Reports

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Endometriosis is a gynecological disease defined as the presence of endometrial tissue outside the uterine cavity, which is caused by various factors. Proteomic analysis of two sets of eutopic endometrial cells collected from the menstrual blood of females with (n=6; n=3) or without (n=6; n=3) endometriosis was performed to identify novel potential biomarkers for endometriosis. The data revealed that samples from endometriosis patients had stem cell characteristics, as they had higher mRNA expression levels of octamer-binding transcription factor 4 (Oct-4), C-X-C chemokine receptor type 4 (CXCR4), SRY-box containing gene 2 (SOX2) and mesenchymal-epithelial transition factor (MET) compared with that of the normal controls. Three proteins, collapsin response mediator protein 2 (CRMP2), ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1) and myosin regulatory light polypeptide 9 (MYL9), were simultaneously identified from the two sets of samples from females with or without endometriosis by two-dimensional electrophoresis (2-DE). A difference in CRMP2 expression was confirmed with western blotting. Taken together, the results suggest that CRMP2 plays a role in the pathogenesis of endometriosis.

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Increased telomerase activity and human telomerase reverse transcriptase mRNA expression in the endometrium of patients with endometriosis

Abstract: This study showed the overexpression of hTERT mRNA and telomerase activity in the endometrium of endometriosis patients. These finding suggest that replication potential of endometrial cells may have an important role in the pathogenesis of endometriosis.

Cited by 39 publications

References 27 publications

Endometrial expression of telomerase, progesterone, and estrogen receptors during the implantation window in patients with recurrent implantation failure

Endometrial expression of telomerase, progesterone, and estrogen receptors during the implantation window in patients with recurrent implantation failure

Endometrial stem cells in regenerative medicine

Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach

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