Increased Susceptibility of ST2-Deficient Mice to Polymicrobial Sepsis Is Associated with an Impaired Bactericidal Function

Buckley, J. J. C.; Liu, Jing Hua; Li, Chong Hui; Blankson, Siobhan; Wu, Qiong; Redmond, H. P.; Wang, Jiang Huai

doi:10.4049/jimmunol.1003872

Cited by 30 publications

(36 citation statements)

References 44 publications

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“…Moreover, ST2-deficient mice show an increased susceptibility to sepsis due to a default in bactericidal activity. Indeed, an inefficient maturation of phagosomes and a strong repression of NOX2 led to limited production of reactive oxygen species and a default in bactericidal activity (50).…”

Section: The Il-33/st2 Axis and Bacterial Infectionsmentioning

confidence: 99%

Crucial and Diverse Role of the Interleukin-33/ST2 Axis in Infectious Diseases

et al. 2015

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Section: The Il-33/st2 Axis and Bacterial Infectionsmentioning

confidence: 99%

Crucial and Diverse Role of the Interleukin-33/ST2 Axis in Infectious Diseases

et al. 2015

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“…ST2L stimulates, while sST2 has a suppressive role on the Th2 immune response [170,173,174]. Moreover, sST2 down-regulates the inflammatory response through the modulation of TLR expression [176,177]. TLRs are of crucial importance for the recognition of microorganisms and activation of the innate immune response (inflammation) [15,153-158].…”

Section: Discussionmentioning

confidence: 99%

“…This interaction can be interrupted by sST2, which binds IL-33 and suppress its stimulating properties [171]. Soluble ST2 is involved in regulation of the Th1/Th2-associated immune response and modulation of the inflammatory response in the presence of infection [170,173-175], while it has anti-inflammatory properties through negative regulation of TLR-2 and TLR-4 [176,177]. Soluble ST2 has been implicated in several pathological conditions such as allergic asthma [178-183], lung fibrosis [184,185], chronic obstructive pulmonary disease [186], acute myocardial infarction [187,188] and preeclampsia [189,190].…”

Section: Introductionmentioning

confidence: 99%

Soluble ST2, a modulator of the inflammatory response, in preterm and term labor

Stampalija

Chaiworapongsa

Romero

et al. 2013

The Journal of Maternal-Fetal & Neonatal Medicine

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Objective Intra-amniotic infection/inflammation (IAI) is causally linked with spontaneous preterm labor and delivery. The ST2L receptor and its soluble form (sST2) are capable of binding to interleukin (IL)-33, a member of the IL-1 superfamily. Members of this cytokine family have been implicated in the onset of spontaneous preterm labor in the context of infection. Soluble ST2 has anti-inflammatory properties, and plasma concentrations are elevated in systemic inflammation, such as sepsis, acute pyelonephritis in pregnancy and the fetal inflammatory response syndrome. The aims of this study were to examine: 1) whether amniotic fluid concentrations of sST2 change with IAI, preterm, and term parturition; and 2) if mRNA expression of ST2 in the chorioamniotic membranes changes with acute histologic chorioamnionitis in women who deliver preterm. Methods A cross-sectional study was conducted to determine amniotic fluid concentrations of sST2 in: 1) women with preterm labor (PTL) who delivered at term (n=49); 2) women with PTL who delivered preterm without IAI (n=21); 3) women with PTL who delivered preterm with IAI (n=31); 4) term pregnancies not in labor (n=13); and 5) term pregnancies in labor (n=43). The amniotic fluid concentration of sST2 was determined by ELISA. The mRNA expression of ST2 in the chorioamniotic membranes of women who delivered preterm with (n=24), and without acute histologic chorioamnionitis (n=19) was determined by qRT-PCR. Results 1) Patients with PTL who delivered preterm with IAI had a lower median amniotic fluid concentration of sST2 compared to those with PTL who delivered preterm without IAI [median 410 ng/mL, inter-quartile range (IQR) 152-699 ng/mL vs. median 825 ng/mL, IQR 493-1216 ng/mL; p=0.0003] and those with PTL who delivered at term [median 410 ng/mL, IQR 152-699 ng/mL vs. median 673 ng/mL, IQR 468-1045ng/mL; p=0.0003]; 2) no significant differences in the median amniotic fluid concentration of sST2 were observed between patients with PTL who delivered at term and those who delivered preterm without IAI (p=0.4), and between women at term in labor and those at term not in labor (p=0.9); 3) the mean mRNA expression of ST2 was 4-fold lower in women who delivered preterm with acute histologic chorioamnionitis than in those without this lesion (p=0.008). Conclusions The median sST2 amniotic fluid concentration and mRNA expression of ST2 by chorioamniotic membranes is lower in PTL associated with IAI and acute histologic chorioamnionitis than in PTL without these conditions. Changes in the median amniotic fluid sST2 concentration are not observed in preterm and term parturition without IAI. Thus, amniotic fluid sST2 in the presence of IAI behaves differently when compared to sST2 in the plasma of individuals affected by fetal inflammatory response syndrome, acute pyelonephritis in pregnancy, and adult sepsis. Decreased concentrations of sST2 in IAI are likely to promote a pro-inflammatory response, which is important for parturition in the context of infection.

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“…Brunner et al demonstrated that soluble ST2, a marker for Th2 cytokine-producing cells, is increased in sepsis and trauma patients (28). Buckley et al showed in an animal study that ST2-deficient mice exhibit an increased susceptibility to polymicrobial infection with impaired bacterial clearance, because of the defects in phagosome maturation and production ofreactive oxygen species (29). Hoogerwerf et al made a study of 95 patients with severe sepsis and resulted in sustained elevation of serum sST2 levels, which correlates with disease severity and mortality (13).…”

Section: Resultsmentioning

confidence: 99%

Diagnostic Value of 25-Hydroxyvitamin D Level and New Cytokines in Neonatal Sepsis

Çekmez¹,

Aydemir²,

Yıldırım

et al. 2014

Eur J Inflamm

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The aim of this study was to evaluate diagnostic value of 25-hydroxyvitamin D level, Upar, IL-33 and ST2 in comparison with C-reactive protein, TNF-a and Interleukin-6 in neonatal sepsis. A total of 106 term babies were included 20 of whom were the control group. We used only data of high probable sepsis with blood culture positive infants, therefore 46 infants were excluded. Blood was collected from infants from the first day of sepsis (l.value) and 48-72 hours later (2.value). There were significant differences between the controls and sepsis (l.value) for 25-hydroxyvitamin D levels (35±19ng/ml and 69±7.5ng/ml, p=O.Ol), for IL-33 levels (90±34 ng/ml and 412±170 ng/ml, p=O.Ol), for sST21eveis (453±44 ng/ml and 4120±2720ng/ml, p=O.Ol), for sUpar levels (2.1±1.3 ng/ml and 11.4 ± 5.2 ng/ml, p=O.Ol), respectively. There were significant differences between sepsis (l.value) and sepsis (2.value.) with reference to 25-hydroxyvitamin D, IL-33, sST2, and suPAR levels, respectively. In

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Increased Susceptibility of ST2-Deficient Mice to Polymicrobial Sepsis Is Associated with an Impaired Bactericidal Function

Cited by 30 publications

References 44 publications

Crucial and Diverse Role of the Interleukin-33/ST2 Axis in Infectious Diseases

Crucial and Diverse Role of the Interleukin-33/ST2 Axis in Infectious Diseases

Soluble ST2, a modulator of the inflammatory response, in preterm and term labor

Diagnostic Value of 25-Hydroxyvitamin D Level and New Cytokines in Neonatal Sepsis

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