Increased susceptibility of Pseudomonas aeruginosa to ciprofloxacin in the presence of vancomycin

Day, C A; Marceau-Day, M. L.; Day, Donal F.

doi:10.1128/aac.37.11.2506

Cited by 16 publications

(10 citation statements)

References 16 publications

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“…This phenomenon has been reported for P . aeruginosa when exposed to different antibiotics, including treatment with CIP (Day et al ., ; Lee et al ., ).…”

Section: Resultsmentioning

confidence: 97%

Non‐invasive, ratiometric determination of intracellular pH in Pseudomonas species using a novel genetically encoded indicator

Arce-Rodríguez

Volke

Bense

et al. 2019

Microbial Biotechnology

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Summary The ability of Pseudomonas species to thrive in all major natural environments (i.e. terrestrial, freshwater and marine) is based on its exceptional capability to adapt to physicochemical changes. Thus, environmental bacteria have to tightly control the maintenance of numerous physiological traits across different conditions. The intracellular pH (pHi) homoeostasis is a particularly important feature, since the pHi influences a large portion of the biochemical processes in the cell. Despite its importance, relatively few reliable, easy‐to‐implement tools have been designed for quantifying in vivo pHi changes in Gram‐negative bacteria with minimal manipulations. Here we describe a convenient, non‐invasive protocol for the quantification of the pHi in bacteria, which is based on the ratiometric fluorescent indicator protein PHP (pH indicator for Pseudomonas). The DNA sequence encoding PHP was thoroughly adapted to guarantee optimal transcription and translation of the indicator in Pseudomonas species. Our PHP‐based quantification method demonstrated that pHi is tightly regulated over a narrow range of pH values not only in Pseudomonas, but also in other Gram‐negative bacterial species such as Escherichia coli. The maintenance of the cytoplasmic pH homoeostasis in vivo could also be observed upon internal (e.g. redirection of glucose consumption pathways in P. putida) and external (e.g. antibiotic exposure in P. aeruginosa) perturbations, and the PHP indicator was also used to follow dynamic changes in the pHi upon external pH shifts. In summary, our work describes a reliable method for measuring pHi in Pseudomonas, allowing for the detailed investigation of bacterial pHi homoeostasis and its regulation.

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“…This phenomenon has been reported for P . aeruginosa when exposed to different antibiotics, including treatment with CIP (Day et al ., ; Lee et al ., ).…”

Section: Resultsmentioning

confidence: 97%

Non‐invasive, ratiometric determination of intracellular pH in Pseudomonas species using a novel genetically encoded indicator

Arce-Rodríguez

Volke

Bense

et al. 2019

Microbial Biotechnology

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“…In vitro data used to examine aminoglycoside-quinolone combinations also show variation, with some isolates demonstrating synergism and others showing indifference and, in some cases, antagonism [38,41]. There is also in vitro evidence of synergy when ciprofloxacin is combined with vancomycin [42]. However, to our knowledge, there are no data to examine the effectiveness of these combination in humans.…”

Section: Discussionmentioning

confidence: 92%

Pseudomonas aeruginosaCommunity‐Acquired Pneumonia in Previously Healthy Adults: Case Report and Review of the Literature

2000

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We report a case of rapidly fatal Pseudomonas aeruginosa community-acquired pneumonia (CAP) in a previously healthy 67-year-old woman. Eleven published case reports of P. aeruginosa CAP in previously healthy adults are reviewed. According to our review, the mean age of affected patients is 45.3 years. Five patients described in the literature were smokers with a mean smoking history of 40 pack-years. The clinical presentation is nonspecific, and although the pneumonia can be rapidly fatal, only 33% of the patients who were reported died. However, mortality may be independent of treatment within the first 36 hours of presentation. Exposure to aerosols of contaminated water is a risk factor for P. aeruginosa CAP in this population. Pseudomonas CAP should be considered in the differential diagnosis for anyone with a smoking history who presents with rapidly progressive pneumonia. We discuss treatment recommendations that are based on evidence in the currently available literature on the subject.

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“…We then explored whether lysozyme-sensitive mutants also exhibited elevated sensitivity to antibiotic treatments. To examine bacterial responsiveness to antibiotics of diverse classes, we tested vancomycin (Day et al, 1993 ), ceftazidime (O'Callaghan, 1986 ), ampicillin (Ghobashy and Chiori, 1984 ), tobramycin (Hoff et al, 1974 ), and ciprofloxacin (Roy et al, 1983 ), the first three of which target cell wall synthesis. Tobramycin and ciprofloxacin inhibit protein synthesis and DNA replication, respectively.…”

Section: Resultsmentioning

confidence: 99%

A Genetic Screen Reveals Novel Targets to Render Pseudomonas aeruginosa Sensitive to Lysozyme and Cell Wall-Targeting Antibiotics

Lee

et al. 2017

Front. Cell. Infect. Microbiol.

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Pseudomonas aeruginosa is capable of establishing airway infections. Human airway mucus contains a large amount of lysozyme, which hydrolyzes bacterial cell walls. P. aeruginosa, however, is known to be resistant to lysozyme. Here, we performed a genetic screen using a mutant library of PAO1, a prototype P. aeruginosa strain, and identified two mutants (ΔbamB and ΔfabY) that exhibited decrease in survival after lysozyme treatment. The bamB and fabY genes encode an outer membrane assembly protein and a fatty acid synthesis enzyme, respectively. These two mutants displayed retarded growth in the airway mucus secretion (AMS). In addition, these mutants exhibited reduced virulence and compromised survival fitness in two different in vivo infection models. The mutants also showed susceptibility to several antibiotics. Especially, ΔbamB mutant was very sensitive to vancomycin, ampicillin, and ceftazidime that target cell wall synthesis. The ΔfabY displayed compromised membrane integrity. In conclusion, this study uncovered a common aspect of two different P. aeruginosa mutants with pleiotropic phenotypes, and suggests that BamB and FabY could be novel potential drug targets for the treatment of P. aeruginosa infection.

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Increased susceptibility of Pseudomonas aeruginosa to ciprofloxacin in the presence of vancomycin

Cited by 16 publications

References 16 publications

Non‐invasive, ratiometric determination of intracellular pH in Pseudomonas species using a novel genetically encoded indicator

Non‐invasive, ratiometric determination of intracellular pH in Pseudomonas species using a novel genetically encoded indicator

Pseudomonas aeruginosaCommunity‐Acquired Pneumonia in Previously Healthy Adults: Case Report and Review of the Literature

A Genetic Screen Reveals Novel Targets to Render Pseudomonas aeruginosa Sensitive to Lysozyme and Cell Wall-Targeting Antibiotics

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