Increased stress parameter synthesis in the yeast Saccharomyces cerevisiae after treatment with 4‐hydroxy‐2‐nonenal¹

Abstract

The metabolism of glutathione (GSH), a marker of oxidative stress and trehalose, a rather general physiological stress marker, was examined in exponentially growing Saccharomyces cerevisiae cells after treatment with 4-hydroxynonenal (HNE). GSH was entirely depleted within a 2 h incubation with 250 |iM HNE. After removal of the aldehyde it was replenished by de novo synthesis leading to an overshooting GSH level, which later decreased to the basal level. In addition, trehalose was elevated 4-fold in HNE-treated yeast cells compared to control cells. We conclude that increased GSH levels upon HNE treatment are a general phenomenon of eukaryotic cells to ensure protection and survival during further harsh conditions. Furthermore, we have discovered a new indication for the stress marker trehalose in S. cerevisiae.

Saccharomyces cerevisiae Expresses Three Phospholipid Hydroperoxide Glutathione Peroxidases

“…DISCUSSION A relationship between cellular glutathione and the resistance of yeast to certain products of lipid peroxidation is known (31,32). This study reveals new functions that may establish part of this relationship.…”

Ultrastructural analysis of HNE‐treated Saccharomyces cerevisiae cells reveals fragmentation of the vacuole and an accumulation of lipids in the cytosol

“…Selected time points of 2 h and 11 days after incubation in presence of HNE were of interest to us. The ®rst time-point is identical to the generation time of S. cerevisiae and a series of previous studies 3,8,12 used this period to evaluate the biochemical response to HNE in yeast. The second timepoint corresponds to the resumption of proliferation of surviving cells, subsequent to the degeneration and removal of the aldehyde.…”

4-Hydroxynonenal As a Biological Signal: Molecular Basis and Pathophysiological Implications

“…Although HNE elicited the induction of both GCS subunits, the use of emetine, a protein synthesis inhibitor, resulted in the block of the increase in GCS regulatory subunit mRNA only (Liu et al, 1998). De novo synthesis of GSH (after initial depletion) was also observed after treatment with rather high doses of HNE (250 liM) in the yeast Saccharomyces cerevisiae (Wonisch et al, 1997).…”