Increased sodium-dependent D-glucose transport in the jejunal brush-border membrane of spontaneously hypertensive rat

Vázquez, Carmen M.; Coleto, Rocío; Zanetti, Rosana; Ruı́z-Gutı́errez, Valentina

doi:10.1007/s004240050140

Cited by 6 publications

(6 citation statements)

References 33 publications

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“…Moreover, initial observations by Parenti et al (21) reported that Na ϩ -dependent glucose transport was faster in Milan hypertensive rats compared with normotensive rats. This report has been further supported by recent observations which suggested that the increased glucose transport could be related to increased transporter density in renal tissue from DS rats (14) or jejunal tissue from SHR (30). These data might suggest that some of the changes in cellular Na ϩ transport observed in hypertension might result from a modification of Na ϩ -dependent glucose transport.…”

supporting

confidence: 84%

“…Moreover, recently it has been reported that Na ϩ -coupled glucose transport through SGLT1 activity is increased in renal BBMV from high-salt-loaded DS rats (14). Kinetic studies of Na ϩ -dependent glucose uptake in jejunal BBMV from SHR and WKY suggested that enhancement in glucose transport in SHR could be due to an increase in the number of transport molecules (30).…”

Section: Discussionmentioning

confidence: 99%

“…Increased Na ϩ -dependent glucose transport has been suggested to be due to several cellular mechanisms, such as increased phosphorylation of the cotransporter (30) or induction of trafficking of the Na ϩ -glucose cotransporter from an intracellular pool into the BBMV (5). Thus to investigate the mechanism by which renovascular hypertension might regulate the increase in Na ϩ -dependent glucose uptake, kinetics studies were performed as well as mRNA and protein expression in BBMV from hypertensive and normotensive rats.…”

Section: Discussionmentioning

confidence: 99%

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Angiotensin II-dependent increased expression of Na⁺-glucose cotransporter in hypertension

Bautista¹,

Manning²,

Martínez³

et al. 2004

American Journal of Physiology-Renal Physiology

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Glucose uptake is increased in hypertension. Thus we investigated Na+-glucose cotransporter (SGLT2) activity and expression in proximal tubules from renovascular hypertensive rats. Sham-operated rats, aortic coarctation rats, and aortic coarctation rats treated with either ramipril (2.5 mg.kg-1.day-1 for 21 days) or losartan (10 mg.kg-1.day-1 for 21 days) were used. Na+-dependent glucose uptake was measured in brush-border membrane vesicles (BBMV). Vmax in BBMV from hypertensive rats was greater compared with those from normotensive rats (3 +/- 0.2 vs. 1.5 +/- 0.1 nmol.mg protein-1.min-1) without a change in Km. Renal immunostaining was greater, and Western blot analysis and RT-PCR showed a higher expression of SGLT2 in hypertensive rats than in normotensive rats (1,029 +/- 71 vs. 5,003 +/- 292, 199 +/- 15 vs. 95 +/- 10, and 1.4 +/- 0.2 vs. 0.3 +/- 0.1 arbitrary units, respectively). In rats treated with either ramipril or losartan, Vmax decreased to 2.1 +/- 0.3 and 1.8 +/- 0.4 nmol.mg protein-1.min-1, respectively, as well as did the intensity of immunostaining and levels of protein and mRNA. We suggest that in renovascular hypertension, angiotensin II induced SGLT2 via the AT1 receptor, which was evidenced at both the functional and expression levels, probably contributing to increased absorption of Na+ and thereby to the development or maintenance of hypertension.

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supporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Angiotensin II-dependent increased expression of Na⁺-glucose cotransporter in hypertension

Bautista¹,

Manning²,

Martínez³

et al. 2004

American Journal of Physiology-Renal Physiology

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“…The experiments on D‐glucose transport were performed in vesicles whose volume was similar across all three experimental groups. In all samples used for the study of transport kinetics, the accumulation ratio for 100 μM D‐glucose was about 10‐fold, similar to that reported for other transport studies in rats (17). The kinetic study revealed that the changes in transport in response to dietary modifications affected the V max without modifying the K m , as observed in most adaptive responses (22).…”

Section: Discussionsupporting

confidence: 85%

Dietary Unsaturated Long‐Chain Fatty Acids Modify D‐Glucose Absorption in Weaning Rats

Vicario,

Pedragosa,

Rivero

et al. 2005

J. pediatr. gastroenterol. nutr.

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Objective:The authors evaluated the effects of dietary long‐chain polyunsaturated fatty acids on D‐glucose absorption in weaning rats.Methods:Pups were born from control mothers fed a diet containing (per kg of total fatty acids) 280 g of saturated fatty acids, 496 g of monounsaturated fatty acids and 222 g of polyunsaturated fatty acids or from mothers fed a diet containing a high proportion of saturated fatty acids (920 g/kg) and a low proportion of unsaturated fatty acids (low‐unsaturated fatty acid, 80 g/kg), initiated 2 weeks before mating and continued throughout pregnancy. When pups from low‐unsaturated fatty acid mothers were 15 days old, they were subdivided into two groups: one control (low‐unsaturated fatty acid‐C) and one fed a long‐chain polyunsaturated fatty acid supplement rich in arachidonic acid and docosahexaenoic acid (low‐unsaturated fatty acid‐S) until weaning. At day 21, the kinetics of D‐glucose absorption was studied in brush‐border membrane vesicles from the jejunoileal segment.Results:The maximal transport rate (Vmax) of glucose in the low‐unsaturated fatty acid‐C and low‐unsaturated fatty acid‐S groups was higher than in control rats: 160 and 130 versus 98 pmol/(mg protein·s), respectively (P < 0.05). Rats fed the low‐unsaturated fatty acid diet had a lower diffusion constant (Kd) than control rats did: 21.6 and 29.2 nL/(mg protein·s), respectively (P < 0.05). However, rats receiving the long‐chain polyunsaturated fatty acid supplement and control rats had similar Kd values.Conclusion:These results indicate that dietary long‐chain polyunsaturated fatty acid supplementation can restore, in part, the kinetic characteristics of intestinal D‐glucose absorption in pups from mothers maintained on a low‐unsaturated fatty acid diet.

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“…Abnormalities of a number of transport systems have been described in essential hypertension [33][34][35], but…”

Section: Research Articlesmentioning

confidence: 99%

Increased Na + -H + exchanger activity in the ileal brush-border membrane of spontaneously hypertensive rats

Vázquez

Coleto

Zanetti

et al. 1997

Cellular and Molecular Life Sciences (CMLS)

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In the present study, we have examined the intestinal Na+ transport, through the Na+)-H+ exchanger in ileal brush-border membrane vesicles (BBMV) isolated from spontaneously hypertensive rats (SHR), and normotensive Wistar Kyoto (WKY) rats as a control group. Na+ uptake into ileal BBMV was stimulated the presence of a proton gradient (pH 5.5 inside/pH 7.5 outside) in SHR and WKY rats, resulting in a transient accumulation (overshoot) in both groups of rats. No overshoot was observed in the absence of a pH gradient. The magnitude of the accumulation was significantly higher in SHR than in WKY rats. Uptake of Na+ at equilibrium was identical in the presence and the absence of a proton gradient and was not changed in SHR. The use of amiloride inhibited pH gradient-driven Na+ uptake in a dose-dependent manner with a Ki of 90 microM and 100 microM for SHR and WKY rats, respectively. The relationship between proton gradient-driven Na+ uptake and external Na+ concentration was saturable and conformed to Michaelis-Menten kinetics in both SHR and WKY rats. Lineweaver-Burk analysis of the pH gradient-driven Na+ uptake indicated values of Vmax that were significantly increased in SHR compared to WKY rats (11.4 +/- 0.55 nmol/mg/8 s vs. 4.96 +/- 0.78 nmol/mg/8 s for SHR and WKY rats, respectively). In contrast, similar K(m) for Na+ were found between SHR and WKY rats (4.0 +/- 0.2 mM vs. 4.9 +/- 0.6 mM for SHR and WKY rats, respectively). These studies show derangement in ileal BBMV Na+ transport of SHR, which is characterized by increased Na(+)-H+ exchanger activity.

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Increased sodium-dependent D-glucose transport in the jejunal brush-border membrane of spontaneously hypertensive rat

Cited by 6 publications

References 33 publications

Angiotensin II-dependent increased expression of Na⁺-glucose cotransporter in hypertension

Angiotensin II-dependent increased expression of Na⁺-glucose cotransporter in hypertension

Dietary Unsaturated Long‐Chain Fatty Acids Modify D‐Glucose Absorption in Weaning Rats

Increased Na + -H + exchanger activity in the ileal brush-border membrane of spontaneously hypertensive rats

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Increased sodium-dependent D-glucose transport in the jejunal brush-border membrane of spontaneously hypertensive rat

Cited by 6 publications

References 33 publications

Angiotensin II-dependent increased expression of Na+-glucose cotransporter in hypertension

Angiotensin II-dependent increased expression of Na+-glucose cotransporter in hypertension

Dietary Unsaturated Long‐Chain Fatty Acids Modify D‐Glucose Absorption in Weaning Rats

Increased Na + -H + exchanger activity in the ileal brush-border membrane of spontaneously hypertensive rats

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Angiotensin II-dependent increased expression of Na⁺-glucose cotransporter in hypertension

Angiotensin II-dependent increased expression of Na⁺-glucose cotransporter in hypertension