Increased Sister Chromatid Exchange in Peripheral Blood Lymphocytes from Humans Exposed to Pesticide: Evidence Based on a Meta-analysis

Abstract

Background: Sister chromatid exchange (SCE) in human peripheral blood lymphocytes is one of the most extensively studied biomarkers employed to evaluate genetic damage subsequent to pesticide exposure. Objective: To estimate the pooled levels of SCE in human peripheral blood lymphocytes among population exposed to pesticide. Materials and Methods: Meta-analysis on the association between SCE frequency and pesticide exposure was performed with STATA 10.0 software package and Review Manager 5.0.24 in this study. Results: The overall means of SCE were 7.88 [95% confidence intervals (95%CI): 6.71-9.04] for exposure group and 6.05 (95%CI: 5.13-6.95) for controls, respectively. There was statistically significant difference in the SCE frequency in human peripheral blood lymphocytes between pesticide-exposed groups and control groups, and the summary estimate of weighted mean difference was 1.69 (95%CI: 1.01-2.38). We also observed that pesticide-exposed population had significantly higher SCE frequency than control groups among smokers, nonsmokers, pesticide applicator, pesticide producer, other exposure population and Asian population in stratified analyses. Conclusions: Data indicate that the SCE frequency in human peripheral blood lymphocytes might be an indicator of early genetic effects for pesticide-exposed populations. s Keywords: Sister chromatid exchange -pesticide -peripheral blood lymphocytes -meta-analysis

Study of commonly used organophosphate pesticides that induced oxidative stress and apoptosis in peripheral blood lymphocytes of rats

“…11 It has been reported that OP pesticides induce chromosomal aberration, sister chromatid exchange, and micronucleus frequency in humans (occupationally or accidentally exposed to OP pesticide) peripheral blood lymphocytes and rat bone marrow cells. 12,13 It is also reported that OP pesticides induce genetic damage in erythrocytes of amphibian tadpoles. 14,15 There are only a few reports on acute exposure.…”

Analysis of chromosome damage by sister chromatid exchange (SCE) and redox homeostasis characterization on sheep flocks from Sardinian pasturelands

“…Cytogenetic tests represent direct and sensitive methods that have been used for detecting DNA damages in chromosomes for the bio-monitoring of different species (Penders et al, 2012;Wójcik and Smalec, 2013;Yang et al, 2014). In particular, SCE is a short-term test for the detection of reciprocal exchanges of DNA between two sister chromatids, involving DNA breakage and subsequent re-junction.…”