Increased serum-soluble interleukin-2 receptor in burn patients: characterization and effects on the immune system

Jobin, Nathalie; Garrel, Dominique R.; Bernier, Jacques

doi:10.1016/s0198-8859(99)00133-0

Cited by 14 publications

(13 citation statements)

References 74 publications

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“…Unfortunately, speculation regarding the reason for this inflammatory state is hampered by the lack of a thorough understanding of cytokine interactions during sepsis. The serum sIL-2R concentration is commonly used as an index for in vivo immune system activation and as a biomarker for identifying patients with severe injury and sepsis who are at risk for developing septic shock [25][26][27] . In our study, treatment with the anti-RAGE antibody decreased the level of sIL-2R, as expected.…”

Section: Discussionmentioning

confidence: 99%

Anti-RAGE antibody ameliorates severe thermal injury in rats through regulating cellular immune function

et al. 2014

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Aim: The receptor of advanced glycation end products (RAGE) participates in a variety of pathophysiological processes and inflammatory responses. The aim of this study was to investigate the therapeutic potential of an anti-RAGE neutralizing antibody for severe thermal injury in rats, and to determine whether the treatment worked via modulating cellular immune function. Methods: Full-thickness scald injury was induced in Wistar rats, which were treated with the anti-RAGE antibody (1 mg/kg, iv) at 6 h and 24 h after the injury. The rats were sacrificed on d 1, 3, 5, and 7. Blood and spleen samples were harvested to monitor organ function and to analyze dendritic cell (DC) and T cell cytokine profiles. The survival rate was analyzed up to d 7 after the injury. Results: Administration of the antibody significantly increased the 7 d survival rate in thermally injured rats (6.67% in the model group; 33.33% in anti-RAGE group). Treatment with the antibody also attenuated the multiple organ dysfunction syndrome (MODS) following the thermal injury, as shown by significant decreases in the organ dysfunction markers, including serum ALT, AST, blood urea nitrogen, creatinine and CK-MB. Moreover, treatment with the antibody significantly promoted DC maturation and T cell activation in the spleens of thermally injured rats. Conclusion: Blockade of the RAGE axis by the antibody effectively ameliorated MODS and improved the survival rate in thermally injured rats, which may be due to modulation of cellular immune function.

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Section: Discussionmentioning

confidence: 99%

Anti-RAGE antibody ameliorates severe thermal injury in rats through regulating cellular immune function

et al. 2014

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“…Burn patients display severe alterations of the immune system, mainly in IL-2, IL-6, and the soluble forms of IL-2 and CD25 receptors (Jobin et al, 2000;Peteiro-Cartelle et al, 1999). Interactions between the pineal gland and the immune system are bidirectional, as interleukins and cytokines affect melatonin synthesis and release (Withyachumnarnkul et al, 1990).…”

Section: Discussionmentioning

confidence: 99%

Long-Term Alteration of Daily Melatonin, 6-Sulfatoxymelatonin, Cortisol, and Temperature Profiles in Burn Patients: A Preliminary Report

Pina

Brun

Tissot

et al. 2010

Chronobiology International

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Melatonin, which shows a robust nycthemeral rhythm, plays the role of an endogenous synchronizer, able to stabilize and reinforce circadian rhythms and maintain their mutual phase relationships. Additionally, melatonin is a potent antioxidant and displays immunological properties. Because free radical generation, immune dysfunction, and sleep and metabolic disorders are involved in the short- and long-term pathophysiology of the burn syndrome, we undertook the study of daily urine melatonin, 6-sulfatoxymelatonin (aMT6s, the main hepatic melatonin metabolite), and cortisol variations plus temperature profiles in burn patients using a non-invasive protocol. Eight patients (6 males, 2 females) were studied on three occasions after admission to the intensive care unit (early session: days 1 to 3; intermediate session: day 10; late session: days 20 to 30). Melatonin, aMT6s, and free cortisol levels were determined in urine samples collected at 4 h intervals over a continuous 24 h span. Core temperature was recorded daily. Controls consisted of healthy subjects in the same age range. Cosinor analysis of the data provided an evaluation of mesor, amplitude, and acrophase of circadian rhythms. Also, we calculated day (D), night (N), and 24 h hormone excretions, N/D ratio for melatonin and aMT6s, and D/N ratio for cortisol. These data were analyzed using Kruskal-Wallis test followed by multiple comparisons. Cosinor analysis did not detect a circadian rhythm in melatonin, aMT6s, or cortisol in any of the three sessions. D melatonin excretion displayed a major increase, resulting in a decreased N/D melatonin ratio, and the melatonin mesor (24 h mean) was increased in the early session, compared with controls. For aMT6s, only the early N/D ratio was decreased, and the mesor of the intermediate session increased. These results were not the consequence of hepatic and/or kidney alteration, as the patients' hepatic and renal parameters were in the normal range. The D and N melatonin/aMT6s ratios of controls and patients were similar, and the aMT6s profiles were superimposed on the melatonin ones, mainly during the day. The D, N, and 24 h cortisol values were increased in all sessions, except for the D level of the early session. The consistently increased mesors in the three sessions provided confirmation. The core temperature profiles were abnormal in all three sessions, mainly during the night, although there was a tendency toward normalization with time. The individual mesors were consistently increased compared with controls. Globally, the abnormalities we report could participate in the pathophysiology of short- and long-term alterations observed in burn syndrome, especially disturbances of sleep, metabolism, and immune function.

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“…IL-2 in plasma increased and may have exacerbated the elevation of IFN-␥ levels in these patients. 30 TNF-␣ and IL-10 plasma levels may have been increased by the presence of Fragmin, 31 which was given to the patients prophylactically. Because a number of the patients were obese, another caveat was that TNF-␣ and IL-6 may have been increased in some of the obese patients because there has been a correlation with an increase in these cytokines with truncal fat mass irrespective of age and gender.…”

Section: Discussionmentioning

confidence: 99%

Circulating Endothelial Cell Levels Correlate with Proinflammatory Cytokine Increase in the Acute Phase of Thermal Injury

Kowal-Vern¹,

Webster²,

Rasmasubban³

et al. 2005

Journal of Burn Care & Rehabilitation

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Circulating endothelial cells (CECs) are increased in sickle cell disease, myocardial infarction, and acute lung injury. The purpose of this study was to determine whether CECs are a prognosticating marker for the development of pneumonia in burn patients with/without inhalation injury in addition to their relationship to proinflammatory cytokines. There were 24 patients: 6 with inhalation injury, 5 with burn only,and 13 with burn plus inhalation injury. CECs were measured by anchored cytometry (Clarient ChromaVision, San Juan Capistrano, CA). In addition, plasma levels of tumor necrosis factor-alpha, interferon-gamma, and interleukins (IL)-10, IL-6, IL-4, and IL-2 were compared with CEC levels. Patients with inhalation injury had a significant (P < .001) paucity of CECs compared with the thermally injured with inhalation. There was a statistically significant increase in inteferon-gamma, tumor necrosis factor-alpha, and IL-6, IL-4, and IL-2 compared with control patients (P < .01), with a concomitant increase in the number of CECs. The numbers of CEC levels did not prognosticate which patients would develop pneumonia. Burn patients with/without inhalation injury had concurrent increase in CECs and proinflammatory cytokines during the acute phase of injury.

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Increased serum-soluble interleukin-2 receptor in burn patients: characterization and effects on the immune system

Cited by 14 publications

References 74 publications

Anti-RAGE antibody ameliorates severe thermal injury in rats through regulating cellular immune function

Anti-RAGE antibody ameliorates severe thermal injury in rats through regulating cellular immune function

Long-Term Alteration of Daily Melatonin, 6-Sulfatoxymelatonin, Cortisol, and Temperature Profiles in Burn Patients: A Preliminary Report

Circulating Endothelial Cell Levels Correlate with Proinflammatory Cytokine Increase in the Acute Phase of Thermal Injury

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