Increased serial levels of platelet‐derived growth factor using hypoxic mesenchymal stem cell‐conditioned medium to promote closure acceler‐ ation in a full‐thickness wound

Abstract

The healing process of a full‐thickness wound involves a complex cascade of cellular responses to reverse skin integrity formation. These processes require growth factors, particularly platelet‐derived growth factor (PDGF). Conversely, hypoxic mesenchymal stem‐cell‐conditioned medium (HMSC‐CM)‐contained growth factors notably contribute to acceleration of wound healing. This study aims to investigate the role of HMSC‐CM in controlling the serial levels of PDGF associated with accelerated wound closure in full‐thickness wounds. Twenty male Wistar rats with full‐thickness wounds were developed as animal models. The animals were randomly assigned to four groups, comprising two treatment groups (treated using HMSC‐CM at a high dose as P1 and at a low dose as P2), a control group (administration of base gel), and sham group (healthy group). PDGF levels were examined using an enzyme‐linked immunosorbent assay. Using ImageJ software, wound closure percentages were determined photographically. The study showed that there was a significant increase in PDGF levels on days 3 and 6 after HMSC‐CM treatment, followed by a decrease in PDGF levels on day 9. In line with these findings, wound closure percentage also increased significantly on days 6 and 9. In the rat model, HMSC‐CM administration may promote acceleration of wound closure by increasing serial PDGF levels in the full‐thickness wound.

Effect of Secretome-Hypoxia Mesenchymal Stem Cells on Regulating SOD and MMP-1 mRNA Expressions in Skin Hyperpigmentation Rats

“…We assume that S-HMSC may increase MMP-1 through TGF-β1, IL-1, and IL-6 which are inhibitors of the MMP-1 enzyme synthesis pathway. Previous studies have demonstrated that increased collagen production by fibroblasts may lead to increased deposition into the ECM regulated by the cytokine TGF-β1 [37], [38], [39]. Interestingly, other previous studies have shown that TGF-β1 not only increases collagen production but also inhibits MMPs in the collagen degradation pathway through MMP-3 and MMP-9 [38], [39].…”

“…Previous studies have demonstrated that increased collagen production by fibroblasts may lead to increased deposition into the ECM regulated by the cytokine TGF-β1 [37], [38], [39]. Interestingly, other previous studies have shown that TGF-β1 not only increases collagen production but also inhibits MMPs in the collagen degradation pathway through MMP-3 and MMP-9 [38], [39]. TGF-βs bind to two serine/ threonine kinase receptors consisting of TGF-βRI and TGF-βRII [40].…”

Secretome Hypoxia Mesenchymal Stem Cells Inhibited Ultraviolet Radiation by Inhibiting Interleukin-6 through Nuclear Factor-Kappa Beta Pathway in Hyperpigmentation Animal Models

“…S-HMSCs 100 uL and 200 uL were dissolved in 200 mg of water-based gel as P1 and P2, respectively. The sample was applied topically to the dorsal skin one a day at day 14 until day 28 after UVB-irradiation [28].…”

The Combination of Mesenchymal Stem Cells and Bovine Colostrum in Reducing α-SMA Expression and NLR Levels in Wistar Rats After 50% Fibrotic Liver Resection

“…Mesenchymal stem cells (MSCs) have the capacity to self-renew and differentiate into other cell types, thereby facilitating the process of cell regeneration [9], [10], [11], [12], [13]. On the other hand, bovine colostrum has antioxidant properties that can aid in tissue repair [14].…”

“…A previous study reported that MSCs are able to accelerate the liver regeneration process [20]. MSCs will produce several paracrine factors including vascular endothelial growth factor (VEGF), hepatocyte growth factor, insulin growth factor, and interleukin 10 (IL-10) that associated with liver regeneration [11], [12], [21], [22], [23]. In addition, bovine colostrum has a total antioxidant capacity, such as superoxide dismutase, glutathione peroxidase, catalase, Vitamin E, Vitamin C, and β carotene [24].…”