“…More recent techniques are all based on RT-PCR, rather than Northern Blot, and successfully overcome these issues by providing increased sensitivity, speed and length quantification. Several methods, such as RACE-PAT (Salles, Darrow, O'Connell, & Strickland, 1992), and Ligation-Mediated (LM)-PAT (Salles & Strickland, 1995), extension PAT (ePAT) (Janicke, Vancuylenberg, Boag, Traven, & Beilharz, 2012) or splint-mediated PAT (sPAT) (Minasaki, Rudel, & Eckmann, 2014), have been described, and these are slightly different in their technology in the cDNA synthesis step, each having advantages and disadvantages.…”