Increased salt tolerance in Zymomonas mobilis strain generated by adaptative evolution

Fuchino, Katsuya; Bruheim, Per

doi:10.21203/rs.3.rs-25602/v1

Cited by 4 publications

(6 citation statements)

References 23 publications

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“…This was somewhat contrary to what we anticipated, since Zs100 cells underwent long cultivation under constant stress, which could have in uenced its metabolism or growth pro le for better survival. This result is in sharp contrast to our previous experience with Z. mobilis adaptive evolution against abiotic stress, in which 10-20 passages of serial transfer drove strains to gain adapted phenotype [19]. The obtained result here showed that the competition with yeast in the co-culture did not in uence Z. mobilis ethanologenic physiology.…”

Section: Resultscontrasting

confidence: 99%

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An assessment of serial co-cultivation approach for generating novel Zymomonas mobilis strains

Fuchino¹,

Bruheim²

2020

Preprint

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Objective The alphaproteobacterium Zymomonas mobilis is an efficient ethanol producer. By utilizing its distinctive physiological features, Z. mobilis-based biorefinery shows great potential for large scale industrial biofuel production. Serial co-cultivation is an emerging approach that promotes inter-species interactions which can improve or rewire the metabolic features in industrially useful microorganisms by inducing frequent mutations. We applied this method to assess if adaptation to long term co-culture improves or rewires the desirable physiological features of Z. mobilis.Results We performed serial co-culture of Z. mobilis with the baker’s yeast, Saccharomyces cerevisiae. We observed filamentation of Z. mobilis cells in the co-culture, indicating that the Z. mobilis cells were exposed to a selective pressure due to the presence of a competitor. After 50 times of serial transfers, we characterized the generated Z. mobilis strains. The analysis showed that long term co-culture did not drive significant changes in either the growth or profile of excreted metabolites in the generated strains. In line with this, whole genome sequencing of the generated Z. mobilis strains revealed only minor genetic variations from the parental strain. The result indicates that co-culture method needs to be carefully optimized for Z. mobilis strain improvement.

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Section: Resultscontrasting

confidence: 99%

“…Whole genome of the co-cultured Z. mobilis strains was sequenced by GATC re-sequencing service (INVIEW Genome sequencing). The reference sequence was our lab stock Zm6 strain which was previously sequenced [18,19].…”

Section: Whole Genome Sequencingmentioning

confidence: 99%

An assessment of serial co-cultivation approach for generating novel Zymomonas mobilis strains

Fuchino¹,

Bruheim²

2020

Preprint

Self Cite

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show abstract

“…This was somewhat contrary to what we anticipated, since Zs100 cells underwent long cultivation under constant stress, which could have in uenced its metabolism or growth pro le for better survival. This result is in s sharp contrast to our previous experience with Z. mobilis adaptive evolution against abiotic stress, in which 10-20 passages of serial transfer drove strains to gain adapted phenotype [20]. The obtained result here showed that the competition with yeast in the co-culture did not in uence Z. mobilis ethanologenic physiology.…”

Section: Resultscontrasting

confidence: 99%

An Assessment of Serial Co-cultivation Approach for Generating Novel Zymomonas Mobilis Strains

Fuchino¹,

Bruheim²

2020

Preprint

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ObjectiveThe alphaproteobacterium Zymomonas mobilis is an efficient ethanol producer.Utilizingits distinctive physiological features, Z. mobilis-based biorefineryshows a great potential foran industrial biofuel production at large scale.Serial co-cultivation based adaptationthat promotes species-interactionhas been an emerging approach to improveor rewire metabolic featuresinindustrially useful microorganisms by inducing frequent mutations. We applied this methodto assess ifadaptation to longterm co-cultureimprovesor rewire the desirablephysiological features ofZ. mobilis.ResultsWe have performed serial co-culture of Z. mobilis mixed with the baker yeast Saccharomyces cerevisiae. We observed filamentation of Z. mobilis cell in the co-culture, indicating that Z. mobilis cells were stressed due to the presence of competitorand that there appeared to be a selective pressure. After 50 times of serial transfers, we characterized the generated Z. mobilis strains. The analysis showed that long term co-culture did not drive significant changes in growth or excreted metabolites profile of generated strains. In line with this, whole genome sequencing of the generated Z. mobilis strains revealed only minor genetic variations from parental strain. The result indicates that co-culture method should be carefully optimized for Z. mobilis strain improvement.

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“…This was somewhat contrary to what we anticipated, especially in the Z. mobilis cells with S. cerevisiae in 100 g/L glucose, since the Z. mobilis cells in that condition underwent long cultivation under constant stress, which could have influenced its metabolism or growth profile for better survival. This result is in sharp contrast to our previous experience with Z. mobilis adaptive evolution against salt stress, in which 10-20 passages of serial transfer drove the strains to gain adapted phenotype of two times better total ethanol production under the saline condition than that of the parental strain [ 19 ]. The obtained result here showed that the competition with yeast or E. coli in the co-culture did not influence Z. mobilis ethanologenic physiology.…”

Section: Main Textcontrasting

confidence: 94%

Section: Main Textmentioning

confidence: 99%

An assessment of serial co-cultivation approach for generating novel Zymomonas mobilis strains

Fuchino

Bruheim

2020

BMC Res Notes

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Objective The alphaproteobacterium Zymomonas mobilis is an efficient ethanol producer, and Z. mobilis-based biorefinery shows great potential for biofuel production. Serial co-cultivation is an emerging approach that promotes inter-species interactions which can improve or rewire the metabolic features in industrially useful microorganisms by inducing frequent mutations. We applied this method to assess if it improves or rewires the desirable physiological features of Z. mobilis, especially ethanol production. Results We performed serial co-culture of Z. mobilis with the baker’s yeast, Saccharomyces cerevisiae. We observed filamentation of Z. mobilis cells in the co-culture, indicating that the Z. mobilis cells were exposed to stress due to the presence of a competitor. After 50 times of serial transfers, we characterized the generated Z. mobilis strains, showing that long term co-culture did not drive significant changes in either the growth or profile of excreted metabolites in the generated strains. In line with this, whole genome sequencing of the generated Z. mobilis strains revealed only minor genetic variations from the parental strain. 50 generations of Z. mobilis monoculture did not induce morphological changes or any significant genetic variations. The result indicates that the method needs to be carefully optimized for Z. mobilis strain improvement.

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Increased salt tolerance in Zymomonas mobilis strain generated by adaptative evolution

Cited by 4 publications

References 23 publications

An assessment of serial co-cultivation approach for generating novel Zymomonas mobilis strains

An assessment of serial co-cultivation approach for generating novel Zymomonas mobilis strains

An Assessment of Serial Co-cultivation Approach for Generating Novel Zymomonas Mobilis Strains

An assessment of serial co-cultivation approach for generating novel Zymomonas mobilis strains

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