Increased retention of polycyclic aromatic hydrocarbons in soils induced by soil treatment with humic substances

Conte, Pellegrino; Zena, A.; Pilidis, George; Piccolo, Alessandro

doi:10.1016/s0269-7491(00)00101-9

Cited by 108 publications

(59 citation statements)

References 13 publications

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“…Humic substances are known to be naturally occurring surface-active agents capable of increasing the mobility of hydrophobic hydrocarbons in soils (Piccolo, 1994;Rebhun et al, 1996;Verstraete and Devliegher, 5 1996;Janzen et al, 1996;Kastner and Mahro, 1996;lnglesias± Jimenez et al 1997;Ortega±Calvo and Saiz±Jimenez, 1998;Lassen and Carlsen, 1999;Conte et al, 2001). Although it has been shown that phytogenic molecules present in the rizosphere may be usefully applied to enhance the aerobic bioremediation of PCB-contaminated soils Di Gioia, 1998, 2001), nothing is known, to the best of our knowledge, on the eects of HS on the environmental fate of PCBs in contaminated soils.…”

Section: Discussionmentioning

confidence: 99%

Effects of humic substances on the bioavailability and aerobic biodegradation of polychlorinated biphenyls in a model soil

Fava

Piccolo

2001

Biotech & Bioengineering

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The very high hydrophobicity of polychlorinated biphenyls (PCBs) strongly reduces their bioavailability in aged contaminated soils, thus limiting their bioremediation. The biodegradability of PCBs in heavily contaminated soils can be significantly enhanced by soil treatment with surface-active agents. In this work, the effects of naturally occurring surfactants such as humic substances (HS) on the aerobic biodegradation of PCBs in a model soil were studied. The soil was amended with biphenyl (4 g/kg), Fenclor 42 (1,000 mg/kg), the aerobic PCB-biodegrading bacterial co-culture ECO3 (inoculum: 10(8)CFU/mL), and treated in aerobic batch slurry-phase conditions (17.5% w/v) with and without the addition of HS at the rates of 1.5 and 3.0% (w/w). Low PCBs biodegradation and dechlorination yields were observed in the HS-free microcosms, probably as a result of the rapid disappearance of inoculated bacteria. The presence of HS influenced significantly the activity of the specialized biomass and the biodegradation of PCBs in the microcosms. The microcosms that received HS at the 1.5% rate showed a higher persistence of the specialized bacteria and yields of PCB biodegradation and dechlorination about 150 and 100%, respectively, larger than those found for the HS-free microcosms. Lower stimulating effects were observed in the microcosms added with the HS at 3.0% rate. These effects were attributed to an increased solubilization of PCBs in the hydrophobic domains of the humic supramolecular associations and to a different accessibility of PCBs by the specialized bacteria at the different rates of HS addition. Although the slurry-phase treatment generally showed a decrease of the original soil ecotoxicity, the addition of the originally non-toxic HS decreased soil ecotoxicity for the Collembola animal biomarker and increased that towards the Lepidium sativum vegetal biomarker.

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Section: Discussionmentioning

confidence: 99%

Effects of humic substances on the bioavailability and aerobic biodegradation of polychlorinated biphenyls in a model soil

Fava

Piccolo

2001

Biotech & Bioengineering

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“…As a result, the US Environmental Protection Agency (US EPA) and the European Community placed PAHs in the priority pollutant list (Wild and Jones, 1995). Unfortunately, PAHs pollutant streams that enter the environment have not been sufficiently stemmed; indeed their intensity continues to increase, mainly due to the rising demand for fossil fuels (Conte et al, 2001). Being both chemically very stable while exhibiting a high hydrophobicity ensures that PAHs are effectively adsorbed onto soil particles and in particular soil organic matter (Means et al, 1980).…”

Section: Introductionmentioning

confidence: 99%

Effect of pyrene on denitrification activity and abundance and composition of denitrifying community in an agricultural soil

Guo

Deng

Qiao

et al. 2011

Environmental Pollution

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a b s t r a c tToxicity of pyrene on the denitrifiers was studied by spiking an agricultural soil with pyrene to a series of concentrations (0e500 mg kg À1 ) followed by doseeresponse and dynamic incubation experiments. Results showed a positive correlation between potential denitrification activity and copy numbers of denitrifying functional genes (nirK, nirS and nosZ), and were both negatively correlated with pyrene concentrations. Based on the comparison of EC 50 values, denitrifiers harboring nirK, nirS or nosZ gene were more sensitive than denitrification activity, and denitrifiers harboring nirS gene were more sensitive than that harboring nirK or nosZ genes. Seven days after spiking with EC 50 concentration of pyrene, denitrifiers diversity decreased and community composition changed in comparison with the control. Phylogenetic analyses of three genes showed that the addition of pyrene increased the proportion of Bradyrhizobiaceae, Rhodospirillales, Burkholderiales and Pseudomonadales. Some species belonging to these groups were reported to be able to degrade PAHs.

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“…Furthermore, few efforts have been made to improve its performances and to investigate the possibility of using environmental compatible pollutant-mobilizing agents instead of chemical surfactants. Some biogenic products, such as cyclodextrins (Fava et al, , 2003McCray and Brusseau, 1998;Boving et al, 1999;Reid et al, 2000;Sheremata and Hawari, 2000;Morillo et al, 2001;Ko and Yoo, 2003), dissolved humic substances (Iglesias-Jimenez et al, 1997;Conte et al, 2001;Fava and Piccolo, 2002), and microbial surfactants (Noordman et al, 1998;Rosenberg and Ron, 1999;Christofi and Ivshina, 2002), have been recently found to be promising mobilizing agents for hydrophobic aromatic pollutants in different soils, as they conjugated marked pollutantmobilizing effects to nontoxicity and biodegradability. Thus, the use of these agents in lieu of synthetic surfactants may contribute to improving the environmental compatibility of the washing technology, which combines good pollutant recovery yields with few or no negative impacts on the soil native structure and on the biotreatability of the resulting wastewaters.…”

Section: Introductionmentioning

confidence: 99%

Effects of cyclodextrins, humic substances, and rhamnolipids on the washing of a historically contaminated soil and on the aerobic bioremediation of the resulting effluents

Berselli

Milone

Canepa

et al. 2004

Biotech & Bioengineering

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Nontoxic and biodegradable pollutant-mobilizing agents, instead of chemical surfactants, were tested in the washing of an actual-site chloroaromatic-contaminated soil. A soil historically contaminated by chlorinated anilines and benzenes, thiophenes and several polycyclic aromatic hydrocarbons was subjected to washing by suspending it (15% w/v) in water or in water with 1.0% (w/v) beta-clodextrin (beta-CD), hydroxypropyl-beta-cyclodextrin (HP-beta-CD), rhamnolipid (RL), dissolved humic substances (HS), or Triton X-100 (TX) in shaken batch reactors for 24 hr. The resulting wastewaters were amended with nutrients and treated aerobically in shaken reactors for 65 days. The biogenic agents markedly enhanced (by 237%, beta-CD; 265%, HP-beta-CD; 400%, RL; 566%, HS) the capability of water of eluting organic pollutants from the soil. TX enhanced the overall pollutant removal by about 660%; however, a lower depletion of the initial soil ecotoxicity, along with a more extensive impact on the soil organic matter, was observed. Furthermore, TX adversely affected the bioremediation of the resulting effluent by apparently inducing a premature decrease of specialized bacterial biomass. By contrast, the biogenic agents, and in particular HS and RL, sustained the biodegradation and dechlorination of pollutants by apparently enhancing the availability of specialized bacteria in the reactors. Thus, the biogenic agents proposed here seem to be promising nontoxic and nonaggressive soil washing agents for the integrated physicochemical (washing) and biological (aerobic posttreatment) restoration of poorly bioremediable (chloro) organics-contaminated soils.

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Increased retention of polycyclic aromatic hydrocarbons in soils induced by soil treatment with humic substances

Cited by 108 publications

References 13 publications

Effects of humic substances on the bioavailability and aerobic biodegradation of polychlorinated biphenyls in a model soil

Effects of humic substances on the bioavailability and aerobic biodegradation of polychlorinated biphenyls in a model soil

Effect of pyrene on denitrification activity and abundance and composition of denitrifying community in an agricultural soil

Effects of cyclodextrins, humic substances, and rhamnolipids on the washing of a historically contaminated soil and on the aerobic bioremediation of the resulting effluents

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