Increased raw poultry meat colonization by extended spectrum beta-lactamase-producing Escherichia coli in the south of Spain

Egea, Pilar; López-Cerero, Lorena; Torres, Eva; Gómez-Sánchez, María del Carmen; Serrano, Lara; Navarro, M.D.; Rodríguez‐Baño, Jesús; Pascual, Álvaro

doi:10.1016/j.ijfoodmicro.2012.08.002

Cited by 78 publications

(51 citation statements)

References 32 publications

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“…None of the 141 ESBL/ AmpC-producing E. coli isolates from raw chicken imported into the United Kingdom from South America was identified as ST131 (Dhanji et al, 2010). Similarly, Egea et al (Egea et al, 2012) did not detect at all the ST131 clone among ESBL-producing E. coli isolates from 33 raw retail meat samples. Conversely, E. coli ST131 was isolated from seven out of 100 retail chicken meat samples analyzed in Spain: three isolates were resistant to FQ and CTX-M-9 positive; one tested positive for CTX-M-9 and one was resistant to FQ (Mora et al, 2010).…”

Section: Discussionmentioning

confidence: 90%

Extraintestinal pathogenic Escherichia coli sequence type 131 H30-R and H30-Rx subclones in retail chicken meat, Italy

Ghodousi

Bonura

Carlo

et al. 2016

International Journal of Food Microbiology

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Extraintestinal pathogenic Escherichia coli sequence type 131 (ST131), typically fluoroquinolone-resistant (FQ-R) and/or extended-spectrum β-lactamase (ESBL)-producing, has emerged globally. Among clinical isolates, ST131, primarily its H30-R and H30-Rx subclones, accounts for most antimicrobial-resistant E. coli and is the dominant E. coli strain worldwide. We assessed its prevalence and characteristics among raw chicken meat samples on sale in Palermo, Italy. A collection of 237 fluoroquinolone resistant and ESBL/AmpC producing E. coli isolates, which had been isolated from processed retail chicken meat in the period May 2013-April 2015, was analyzed. Established polymerase chain reaction methods were used to define ST131 and its H30 subclones, ESBL, AmpC, and plasmidmediated quinolone resistance (PMQR) determinants. Amplified Fragment Length Polymorphism (AFLP) was performed to assess the relatedness among ST131 isolates. Out of the 237 E. coli isolates, 12 isolates belonged to the phylogenetic group B2. Based on the molecular definition of ExPEC, all isolates were attributed with the status of ExPEC. SNP-PCR results confirmed that nine isolates were ST131. SNP-PCR for H30-R and H30-Rx subclones showed that six and three ExPEC ST131 were positive for H30-R and H30-Rx, respectively. The results of AFLP showed that, except for four isolates grouped into two clusters which proved to be indistinguishable, the isolates under study were genetically heterogeneous. To the best of our knowledge, this is the first report of H30-R and H30-Rx subclones in animal food samples. Our findings appear to support the role of food chain in their transmission to humans.

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Section: Discussionmentioning

confidence: 90%

Extraintestinal pathogenic Escherichia coli sequence type 131 H30-R and H30-Rx subclones in retail chicken meat, Italy

Ghodousi

Bonura

Carlo

et al. 2016

International Journal of Food Microbiology

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“…However, plasmid-mediated AmpCproducing microorganisms were not detected due to the experimental setup (17). In addition, Egea et al (37) reported a contamination of raw poultry meat with ESBL-producing E. coli of 93.3%. Cohen et al (18) also reported that 94% of chicken meat samples harbored at least one E. coli isolate with an ESBL phenotype.…”

Section: Discussionmentioning

confidence: 99%

Longitudinal Monitoring of Extended-Spectrum-Beta-Lactamase/AmpC-Producing Escherichia coli at German Broiler Chicken Fattening Farms

Laube¹,

Friese²,

Salviati³

et al. 2013

Appl Environ Microbiol

130

106

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Antimicrobial resistance of Escherichia coli to modern beta-lactam antibiotics due to the production of extended-spectrum betalactamases (ESBL) and/or plasmid-mediated AmpC beta-lactamases (AmpC) represents an emerging and increasing resistance problem that dramatically limits therapeutic options in both human and veterinary medicine. The presence of ESBL/AmpC genes in commensal E. coli from food-producing animals like broilers may pose a human health hazard. However, there are no data available concerning the prevalence of ESBL/AmpC-producing E. coli in German broiler flocks using selective methods. In this longitudinal study, samples were taken from seven conventional broiler fattening farms at three different times within one fattening period. Various samples originating from the animals as well as from their direct environment in the barn were investigated for the occurrence of ESBL/AmpC-producing E. coli. Average detection levels of 51, 75, and 76% in animal samples collected during the three samplings in the course of the fattening period demonstrate a colonization of even 1-day-old chicks, as well as a continuous significant (P < 0.001) increase in prevalence thereafter. The detection frequencies in housing environmental samples were relatively high, with an increase over time, and ranged between 54.2 and 100%. A total of 359 E. coli isolates were characterized by PCR and partly via the disc diffusion method. This study shows that prevalence of ESBL/AmpC-producing E. coli increases during the fattening period of the broiler flocks examined. Both colonized day-old chicks and contaminated farm environments could represent significant sources of ESBL/AmpC-producing E. coli in German broiler fattening farms.

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“…This result is not particularly surprising, given that attempts to detect E. coli ST131 among ESBL-producing E. coli isolates from retail chicken meat have rarely been successful (35,186,190,191,209). In contrast, two STs commonly identified among ESBL-producing E. coli isolates from chicken meat, ST117 and ST354 (189,209,210), were identified among the dominant clonal groups for French CTX-Mproducing non-ST131 clinical isolates (Table 7) (16).…”

Section: Factors Associated With Clinical Isolates Of E Coli St131mentioning

confidence: 94%

“…coli ST131 also appears to be very rare in foodstuffs of animal origin. Vincent et al reported a single non-ESBL-producing (191). In contrast, E. coli ST131 was isolated from seven retail chicken meat samples from the 100 analyzed in Spain (prevalence, 7%).…”

Section: Animal-and Food-borne E Coli St131mentioning

confidence: 97%

Escherichia coli ST131, an Intriguing Clonal Group

2014

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SUMMARY In 2008, a previously unknown Escherichia coli clonal group, sequence type 131 (ST131), was identified on three continents. Today, ST131 is the predominant E. coli lineage among extraintestinal pathogenic E. coli (ExPEC) isolates worldwide. Retrospective studies have suggested that it may originally have risen to prominence as early as 2003. Unlike other classical group B2 ExPEC isolates, ST131 isolates are commonly reported to produce extended-spectrum β-lactamases, such as CTX-M-15, and almost all are resistant to fluoroquinolones. Moreover, ST131 E. coli isolates are considered to be truly pathogenic, due to the spectrum of infections they cause in both community and hospital settings and the large number of virulence-associated genes they contain. ST131 isolates therefore seem to contradict the widely held view that high levels of antimicrobial resistance are necessarily associated with a fitness cost leading to a decrease in pathogenesis. Six years after the first description of E. coli ST131, this review outlines the principal traits of ST131 clonal group isolates, based on the growing body of published data, and highlights what is currently known and what we need to find out to provide public health authorities with better information to help combat ST131.

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Increased raw poultry meat colonization by extended spectrum beta-lactamase-producing Escherichia coli in the south of Spain

Cited by 78 publications

References 32 publications

Extraintestinal pathogenic Escherichia coli sequence type 131 H30-R and H30-Rx subclones in retail chicken meat, Italy

Extraintestinal pathogenic Escherichia coli sequence type 131 H30-R and H30-Rx subclones in retail chicken meat, Italy

Longitudinal Monitoring of Extended-Spectrum-Beta-Lactamase/AmpC-Producing Escherichia coli at German Broiler Chicken Fattening Farms

Escherichia coli ST131, an Intriguing Clonal Group

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