Increased rate of lipid peroxidation and protein carbonylation in experimental diabetic pregnancy

Cederberg, Jonas; Basu, Samar; Eriksson, Ulf J.

doi:10.1007/s001250051686

Cited by 101 publications

(64 citation statements)

References 32 publications

(36 reference statements)

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“…Interestingly, exogenous treatment with α-tocopherol markedly increased α-tocopherol concentrations in the renal medulla of all treated animals, but was more pronounced in the diabetic animals (increased five-fold). Previous studies have shown that lipid peroxidation, measured as TBARS, and carbonylated proteins are good markers for radical damage to lipids and proteins, respectively [9,46]. An increased concentration of protein carbonyls is closely associated with poor diabetic control and correlates positively to HbA 1c [47].…”

Section: Discussionmentioning

confidence: 99%

Reactive oxygen species cause diabetes-induced decrease in renal oxygen tension

Palm

Cederberg²,

Hansell³

et al. 2003

Diabetologia

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273

288

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Aims/hypothesis. Augmented formation of reactive oxygen species (ROS) induced by hyperglycaemia has been suggested to contribute to the development of diabetic nephropathy. This study was designed to evaluate the influence of streptozotocin (STZ)-induced diabetes mellitus, as well as the effects of preventing excessive ROS formation by α-tocopherol treatment, on regional renal blood flow, oxygen tension and oxygen consumption in anaesthetized Wistar Furth rats. Methods. Non-diabetic and STZ-diabetic rats were investigated after 4 weeks with or without dietary treatment with the radical scavenger DL-α-tocopherol (vitamin E, 5%). A laser-Doppler technique was used to measure regional renal blood flow, whilst oxygen tension and consumption were measured using Clarktype microelectrodes. Results. Renal oxygen tension, but not renal blood flow, was lower throughout the renal parenchyma of diabetic rats when compared to non-diabetic control rats. The decrease in oxygen tension was most pronounced in the renal medulla. Renal cellular oxygen consumption was markedly increased in diabetic rats, predominantly in the medullary region. Diabetes increased lipid peroxidation and protein carbonylation in the renal medulla. Treatment with α-tocopherol throughout the course of diabetes prevented diabetesinduced disturbances in oxidative stress, oxygen tension and consumption. The diabetic animals had a renal hypertrophy and a glomerular hyperfiltration, which were unaffected by α-tocopherol treatment. Conclusions/interpretation. We conclude that oxidative stress occurs in kidneys of diabetic rats predominantly in the medullary region and relates to augmented oxygen consumption and impaired oxygen tension in the tissue. [Diabetologia (2003[Diabetologia ( ) 46:1153[Diabetologia ( -1160

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Section: Discussionmentioning

confidence: 99%

Reactive oxygen species cause diabetes-induced decrease in renal oxygen tension

Palm

Cederberg²,

Hansell³

et al. 2003

Diabetologia

Self Cite

273

288

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“…We hypothesize that the need for GSH availability in alveolar macrophage functioning can be applicable to other conditions in pregnancy that increase oxidative stress for the developing fetus. These include diabetes (48,49), maternal smoking (50), pregnancy-induced hypertension (50 -52), intrauterine growth retardation (53), and preterm premature rupture of membranes (54). Therefore, the potential implica- …”

Section: Fetal Alcohol Impairs Fetal Macrophagementioning

confidence: 99%

Fetal Alcohol Exposure Impairs Alveolar Macrophage Function via Decreased Glutathione Availability

et al. 2005

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Immature function of the alveolar macrophage increases the risk of pulmonary infections in premature newborns. In utero alcohol increases fetal systemic oxidative stress. Because the premature lung is deficient in glutathione (GSH), we hypothesized that chronic in utero alcohol (ethanol) exposure exacerbates the oxidative stress within the developing lung, thereby impairing alveolar macrophage function. Additionally, we evaluated the effects of in vivo and in vitro GSH availability on ethanol-exposed macrophage function. Using a guinea pig model of chronic in utero ethanol exposure, fetal epithelial lining fluid (ELF) and alveolar macrophage GSH were decreased with increased markers of oxidative stress. Ethanol-exposed macrophage exhibited impaired phagocytosis and increased apoptosis compared with gestational control. When the GSH precursor S-adenosyl-methionine (SAM) was added to the maternal drinking water containing ethanol, fetal ELF and macrophage GSH were maintained and ELF oxidative stress diminished. In vivo maternal SAM therapy maintained macrophage phagocytosis and decreased apoptosis. In vitro GSH supplements also improved phagocytosis and viability in both premature and ethanolexposed macrophage. This suggested that in utero ethanol impaired premature macrophage function and viability via decreased GSH availability. Furthermore, GSH supplementation during and after ethanol exposure improved fetal macrophage function and viability. These results add a new dimension to the detrimental effects of fetal alcohol exposure on the developing alveolar macrophage, raising the possibility of GSH therapy to augment premature alveolar macrophage function. Fetal alcohol exposure remains a significant problem in our society. Alcohol abuse and binge drinking by pregnant women has been estimated at an alarming 35% of pregnancies (1-3). Fetal alcohol syndrome or alcohol-related neurodevelopmental disorder has been estimated to occur in ‫0001/1ف‬ pregnancies (4). Because there is a strong relationship between cocaine abuse and concurrent alcohol ingestion (5-7), and both substances may increase the risk of premature delivery (7,8), a significant population of premature infants is exposed to alcohol in utero.In animal models of fetal alcohol exposure, alcohol increases systemic oxidative stress in the developing fetus (9 -14). A decrease in the antioxidant GSH has been demonstrated, particularly in the alcohol-exposed fetal liver (9,15,16). As an essential antioxidant in the body, GSH is normally present in high concentrations in the ELF of the lung (17). The fetal lung is at risk for increased oxidative stress during development, because maturation of antioxidant systems, including GSH, is gestationally dependent (18,19). A reduction in alveolar GSH, as seen in the premature infant, leaves the preterm lung susceptible to increased pulmonary oxidative injury (20,21) and chronic lung disease (18,22). The impact of the oxidative stress of fetal alcohol exposure superimposed on the low GSH environment of the developing lu...

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“…Oxidative stress such as lipid peroxidation and protein oxidation has been shown increased in both insulin-dependent diabetes (IDDM), and non-insulin dependent (NIDDM). [31][32][33] When oxygen free radicals are involved in pathological conditions antioxidants could be effective in attenuating their severity, and employing plant preparations rich in phenolic compounds would be a significant alternative. These findings indicate that some of the phytochemicals present in B. microstachya leaves may contribute in a significant way to the intake of antioxidants and make these low-cost beverages very interesting from a nutritional point of view jointly with other phenolic-rich extracts.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Activities and Free Radical Scavenging Potential of Bauhinia microstachya (RADDI) MACBR. (Caesalpinaceae) Extracts Linked to Their Polyphenol Content

Silva

Behr

Zanotto-Filho

et al. 2007

Biological & Pharmaceutical Bulletin

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Bauhinia belongs to the Caesalpinaceae, a plant family that comprises approximately 250 species.1) Native peoples from tropical regions use Bauhinia leaves and bark as medicine in infusions and other phytotherapic preparations. 2,3)Some native Bauhinia species in Brazil are known as "patade-vaca" (cow's hoof) and are widely used in folk medicine to treat various ailments, mainly infections and diabetes. Some reports have demonstrated positive effects on factors related to diseases and pathological states.2-5) Bauhinia microstachya (RADDI) MACBR. (Caesalpinaceae) is a creeper plant that occurs naturally in southern Brazil where is popularly known as "escada-de-macaco" (monkey's ladder). B. microstachya is often cultivated in small areas and its leaves are used as herbal antidiabetic medicine. Traditionally the leaf infusion or garrafada (a preparation with cachaça-the popular local sugar cane brandy-and leaves) is drunk after meals to help control blood sugar levels and other diabetic disorders. Phytochemical investigations with B. microstachya leaves have identified compounds such as steroidal glycosides, triterpenes, lactones and phenolic compounds, mainly flavonoids. [6][7][8] Plant phenolics form a large group of natural compounds, ubiquitous in the plant kingdom. It is known that these secondary metabolites display a remarkable array of biochemical interactions, probably due to antioxidant properties. 9)These substances may act as potent metal chelators and/or free radical scavengers, 10) however, it has been reported that the performance of these compounds in oxidative systems depends on activity-structure relationships.11,12) As an unfortunate consequence of aerobic life, free radicals and other reactive oxygen species (ROS) are formed by biological redox reactions.13) The role of free radicals reactions in biology and medicine has become an area of intense interest due to their relationship to chronic diseases.13) It is generally accepted that free radicals play an important role in the development of tissue damage and pathological events in living organisms.13) Lipids containing polyunsaturated fatty acids can be oxidized by free radical-mediated reactions. In addition, when oxygen is supplied in excess or their reduction is insufficient, this generates endogenous ROS imbalance with formation of hydroxyl ( · OH) and superoxide (O 2 · Ϫ ) radicals.13) In inflammation and endothelial damage, NO plays a major role as precursor of peroxynitrite (ONOO Ϫ ).14) If the endogenous response system, such as antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), is inadequate in scavenging ROS, damage to important macromolecules can be established and generate cellular oxidative stress.13) In addition, the non-enzymatic antioxidants (such as vitamin C, vitamin E and phenolic compounds found in a vegetable-rich diet) play a significant role in the physiological redox balance together with enzymatic defenses. 13)Considering the increasing interest in antioxidants, the potential antioxidant effects of pheno...

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Increased rate of lipid peroxidation and protein carbonylation in experimental diabetic pregnancy

Cited by 101 publications

References 32 publications

Reactive oxygen species cause diabetes-induced decrease in renal oxygen tension

Reactive oxygen species cause diabetes-induced decrease in renal oxygen tension

Fetal Alcohol Exposure Impairs Alveolar Macrophage Function via Decreased Glutathione Availability

Antioxidant Activities and Free Radical Scavenging Potential of Bauhinia microstachya (RADDI) MACBR. (Caesalpinaceae) Extracts Linked to Their Polyphenol Content

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