Increased RANKL/OPG Ratio and Sclerostin in Patients with Septic Shock

Bergquist, Maria; Andersson, S.; Rylander, Christian; Lindholm, Catharina; Gjertsson, Inger

doi:10.15761/cmid.1000119

Cited by 1 publication

(2 citation statements)

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“…We found that the level of serum RANKL was increased (Figure A) and OPG, P1NP, OCN, Ca 2+ , and ALP levels were decreased in the OVX group (Figure B–D, Figure S1F–H). The ratio of RANKL/OPG played a key role in regulating osteoclast differentiation and affecting the balance between bone formation and bone resorption . Ovariectomy elevated the ratio of RANKL/OPG (Figure C).…”

Section: Resultsmentioning

confidence: 99%

“…The ratio of RANKL/OPG played a key role in regulating osteoclast differentiation and affecting the balance between bone formation and bone resorption. 32 Ovariectomy elevated the ratio of RANKL/OPG (Figure 3C). mEVs could restore bone turnover and improve bone resorption in OVX mice (Figure 3A Furthermore, the effect of mEVs on osteoclast formation was investigated in vitro.…”

Section: Mevs Prevented Bone Lossmentioning

confidence: 94%

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Oral Milk-Derived Extracellular Vesicles Inhibit Osteoclastogenesis and Ameliorate Bone Loss in Ovariectomized Mice by Improving Gut Microbiota

Hao,

Liu,

Zheng

et al. 2024

J. Agric. Food Chem.

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Milk-derived extracellular vesicles can improve intestinal health and have antiosteoporosis potential. In this paper, we explored the effects of bovine raw milk-derived extracellular vesicles (mEVs) on ovariectomized (OVX) osteoporotic mice from the perspective of the gut-bone axis. mEVs could inhibit osteoclast differentiation and improve microarchitecture. The level of osteoporotic biomarkers in OVX mice was restored after the mEVs intervened. Compared with OVX mice, mEVs could enhance intestinal permeability, reduce endotoxin levels, and improve the expression of TNF-α, IL-17, and IL-10. 16S rDNA sequencing indicated that mEVs altered the composition of gut microbiota, specifically for Bacteroides associated with short-chain fatty acids (SCFAs). In-depth analysis of SCFAs demonstrated that mEVs could restore acetic acid, propionic acid, valeric acid, and isovaleric acid levels in OVX mice. Correlation analysis revealed that changed gut microbiota and SCFAs were significantly associated with gut inflammation and osteoporotic biomarkers. This study demonstrated that mEVs could inhibit osteoclast differentiation and improve osteoporosis by reshaping the gut microbiota, increasing SCFAs, and decreasing the level of pro-inflammatory cytokines and osteoclast differentiation-related factors in OVX mice. These findings provide evidence for the use of mEVs as a food supplement for osteoporosis.

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Section: Resultsmentioning

confidence: 99%

Section: Mevs Prevented Bone Lossmentioning

confidence: 94%