Increased proportions of CCR4&lt;sup&gt;+&lt;/sup&gt; cells among peripheral blood CD4&lt;sup&gt;+&lt;/sup&gt; cells and serum levels of allergen-specific IgE antibody in canine chronic rhinitis and bronchitis

Yamaya, Yoshiki; Watari, Toshihiro

doi:10.1292/jvms.14-0338

Cited by 4 publications

(2 citation statements)

References 24 publications

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“…In veterinary medicine, research on antigen-specific IgE has mainly been focused on food allergy [ 71 ], fleabite hypersensitivity [ 72 , 73 ], allergic respiratory diseases [ 74 , 75 , 76 ], and atopic dermatitis [ 53 , 77 , 78 ]. Some private veterinary laboratories propose such allergen-specific IgE testing for clinical application.…”

Section: Humoral In Vitro Testsmentioning

confidence: 99%

In Vitro Research Tools in the Field of Human Immediate Drug Hypersensitivity and Their Present Use in Small Animal Veterinary Medicine

Lavergne

2016

Veterinary Sciences

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Drug hypersensitivity reactions (DHR) are immune-mediated idiosyncratic adverse drug events. Type I DHR are often referred to as “immediate” and involve B lymphocyte-secreted IgE that bind to the membrane of basophils and mast cells, inducing their degranulation. This review presents various in vitro tests that were developed in the field of human type I HS and implemented as clinical diagnostic tools in human cases of immediate DHR. The respective strengths and weaknesses of each test will be discussed in parallel of validation data such as specificity and sensitivity whenever available. Some of them have also been used as diagnostic tools in veterinary medicine, but not in cases of immediate DHR. Most of these diagnostic tools can be categorized into humoral and cellular tests. The former tests measure serum concentrations of factors, such as histamine, tryptase, and drug-specific IgE. The latter assays quantify markers of drug-induced basophil activation or drug-specific lymphocyte proliferation. Pharmacogenetic markers have also been investigated in immediate DHR, but not as extensively as in non-immediate ones. Throughout, practical aspects and limitations of the tests, as well as sensitivity and specificity parameters, will be presented. In addition, the experience of veterinary medicine with these diagnostic tools will be summarized. However, to date, none of them has ever been reported in a veterinary case of type I DHR.

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Section: Humoral In Vitro Testsmentioning

confidence: 99%

In Vitro Research Tools in the Field of Human Immediate Drug Hypersensitivity and Their Present Use in Small Animal Veterinary Medicine

Lavergne

2016

Veterinary Sciences

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“…White blood cells were isolated from peripheral blood using lysing buffer (BD Biosciences; Franklin Lakes, NJ, USA), followed by an incubation with fluorescein isothiocyanate (FITC)‐conjugated mouse anti‐canine CD3 (clone CA17.2A12, Bio‐Rad Laboratories; Hercules, CA, USA), allophycocyanin (APC)‐conjugated rat anti‐canine CD8 (clone YCATE55.9, Bio‐Rad Laboratories) and phycoerythrin (PE)‐conjugated mouse anti‐human CCR4 (clone 1G1, BD Biosciences) monoclonal antibodies in washing buffer at 4°C for 30 min. Antibodies with appropriate isotypes (FITC‐conjugated mouse IgG1, clone MOPC‐21, Bio Legend; APC‐conjugated rat IgG1, clone 43414, R&D Systems; Minneapolis, MN, USA; PE‐conjugated mouse IgG1, clone MOPC‐21, Bio Legend) were used for each sample.…”

Section: Methodsmentioning

confidence: 99%