Increased Proliferation of Neuroblastoma Cells under Fructose Metabolism Can Be Measured by Isothermal Microcalorimetry

Pini, Nicola; Huo, Zihe; Holland‐Cunz, Stefan; Gros, Stephanie J.

doi:10.3390/children8090784

Cited by 2 publications

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More recently, we have been able to link a higher metastatic potential of tumor cells to biophysical properties, such as decreased adherence and increased thermogenesis [ 54 ]. Isothermal microcalorimetry can also be of advantage in evaluating thermogenesis of neuroblastoma cells under stimulation with different nutrient supplies [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Perfusion-Based Bioreactor Culture and Isothermal Microcalorimetry for Preclinical Drug Testing with the Carbonic Anhydrase Inhibitor SLC-0111 in Patient-Derived Neuroblastoma

Huo

Bilang

Supuran

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Neuroblastoma is a rare disease. Rare are also the possibilities to test new therapeutic options for neuroblastoma in clinical trials. Despite the constant need to improve therapy and outcomes for patients with advanced neuroblastoma, clinical trials currently only allow for testing few substances in even fewer patients. This increases the need to improve and advance preclinical models for neuroblastoma to preselect favorable candidates for novel therapeutics. Here we propose the use of a new patient-derived 3D slice-culture perfusion-based 3D model in combination with rapid treatment evaluation using isothermal microcalorimetry exemplified with treatment with the novel carbonic anhydrase IX and XII (CAIX/CAXII) inhibitor SLC-0111. Patient samples showed a CAIX expression of 18% and a CAXII expression of 30%. Corresponding with their respective CAIX expression patterns, the viability of SH-EP cells was significantly reduced upon treatment with SLC-0111, while LAN1 cells were not affected. The inhibitory effect on SH-SY5Y cells was dependent on the induction of CAIX expression under hypoxia. These findings corresponded to thermogenesis of the cells. Patient-derived organotypic slice cultures were treated with SLC-0111, which was highly effective despite heterogeneity of CAIX/CAXII expression. Thermogenesis, in congruence with the findings of the histological observations, was significantly reduced in SLC-0111-treated samples. In order to extend the evaluation time, we established a perfusion-based approach for neuroblastoma tissue in a 3D perfusion-based bioreactor system. Using this system, excellent tissue quality with intact tumor cells and stromal structure in neuroblastoma tumors can be maintained for 7 days. The system was successfully used for consecutive drug response monitoring with isothermal microcalorimetry. The described approach for drug testing, relying on an advanced 3D culture system combined with a rapid and highly sensitive metabolic assessment, can facilitate development of personalized treatment strategies for neuroblastoma.

show abstract

Section: Discussionmentioning

confidence: 99%

Perfusion-Based Bioreactor Culture and Isothermal Microcalorimetry for Preclinical Drug Testing with the Carbonic Anhydrase Inhibitor SLC-0111 in Patient-Derived Neuroblastoma

Huo

Bilang

Supuran

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

Isothermal calorimetry calscreener in the metabolism gauge of human malignant neoplastic cells: a burgeoning nexus in cancer biochemical metrology and diagnostics

Bayode,

Alabi,

Ibisanmi

et al. 2023

Bull Natl Res Cent

View full text Add to dashboard Cite

Background At present, the function of isothermal microcalorimetry (IMC) calscreener in detecting alterations in the metabolic pathways of cancer cells remains unexplored. We disclosed the shortcomings of current screening methods and the need for precise and dependable instruments in the detection and informed treatment of cancer via the IMC in relation to the experimental procedures required to obtain accurate results. Main body of abstract We examined the intricate technical aspects of isothermal calorimetry. The application of the IMC calscreener in cancer research is then discussed in depth, including how it can be used to evaluate the efficacy of treatments, identify metabolic inhibitors, and assess metabolic rates. We also investigated the diagnostic potential of isothermal calorimetry, particularly for early cancer detection and tracing therapy efficacy. Conclusions General findings shed light on the present issues and potential approaches for isothermal calorimetry application in cancer research and diagnosis. We underline the potential for isothermal calorimetry to fundamentally alter how to understand and treat cancer, as well as the need for additional studies to maximize its application in clinical settings. This in turn offers a thorough and fascinating account of the emerging relationship between isothermal calorimetry and cancer biochemistry, as well as its potential to revolutionize cancer detection and therapy.

show abstract

Increased Proliferation of Neuroblastoma Cells under Fructose Metabolism Can Be Measured by Isothermal Microcalorimetry

Cited by 2 publications

References 30 publications

Perfusion-Based Bioreactor Culture and Isothermal Microcalorimetry for Preclinical Drug Testing with the Carbonic Anhydrase Inhibitor SLC-0111 in Patient-Derived Neuroblastoma

Perfusion-Based Bioreactor Culture and Isothermal Microcalorimetry for Preclinical Drug Testing with the Carbonic Anhydrase Inhibitor SLC-0111 in Patient-Derived Neuroblastoma

Isothermal calorimetry calscreener in the metabolism gauge of human malignant neoplastic cells: a burgeoning nexus in cancer biochemical metrology and diagnostics

Contact Info

Product

Resources

About