Increased proliferation and adhesion properties of human dental pulp stem cells in PLGA scaffolds via simulated microgravity

He, Lijuan; Pan, Shuang; Li, Y.; Zhang, L.; Zhang, W.; Hong, Yi; Song, Chao; Niu, Yumei

doi:10.1111/iej.12441

Cited by 27 publications

(22 citation statements)

References 49 publications

(47 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This cell adhesion molecule and extracellular matrix protein is known to be altered when different cell types are exposed to s-µ g conditions 8 , 9 , 18 , 32 , 33 . A reduction was reported in adult retinal pigment epithelium cells and increases in dental pulp stem cells 32 , 33 . During the PFC FTC-133 cells exhibited no change in LAMA1 gene expression after 31 parabolas 18 .…”

Section: Discussionmentioning

confidence: 99%

Thyroid cancer cells in space during the TEXUS-53 sounding rocket mission – The THYROID Project

Kopp

Krüger

Feldmann

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Human follicular thyroid cancer cells (FTC-133) were sent to space via a sounding rocket during the TEXUS-53 mission to determine the impact of short-term microgravity on these cells. To enable cell culture and fixation in real microgravity, an automated experiment container (EC) was constructed. In order to ensure safe cell culture, cell-chambers consisting of polycarbonate (PC) material were used. They were highly biocompatible as proved by measuring cell survival using Annexin V flow cytometry. In the follow-up experiment, FTC-133 cells were sent to space via a sounding rocket and were fixed before and after the microgravity (µg) phase with RNAlater. In addition, cells were tested for reactions on hypergravity (hyper-g) as much as 18 g to determine whether worst case acceleration during launch can have an influence on the cells. We investigated genes belonging to biological processes such as cytoskeleton, cell adhesion, tumor growth, angiogenesis and apoptosis. Pathway analyses revealed central functions of VEGFA and EGF. EGF upregulates aspartate beta-hydroxylase (ASPH) which is influencing CASP3. Hyper-g induced a significant up-regulation of TUBB1, VIM, RDX, CAV1, VEGFA and BCL2. FTC-133 cells grown in an automated EC exposed to µg revealed moderate gene expression changes indicating their survival in orbit.

show abstract

Section: Discussionmentioning

confidence: 99%

Thyroid cancer cells in space during the TEXUS-53 sounding rocket mission – The THYROID Project

Kopp

Krüger

Feldmann

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The dental pulp tissues were digested with 3 mg/ml collagenase type I (Sigma-Aldrich; Merck Millipore, Darmstadt,) and 4 mg/ml dispase (BD Biosciences, San Jose, CA, USA) for 1 h at 37˚C, following which the solution was passed through a 70-µm strainer. The characterizations of the hDPSCs were based on a previous report (17). The cells between passages two and five were used in the following experiments.…”

Section: Methodsmentioning

confidence: 99%

“…Cell-PLGA complex culture. The PLGA scaffolds (Synthecon, Inc., Houston, TX, USA) were pretreated, as previously described (17). The hDPSCs (2x10 6 ) were seeded into each scaffold and cultured in Dulbecco's modified Eagle's medium (DMEM; Hyclone; GE Healthcare Life Sciences, Chalfont, UK) supplemented with 10% fetal bovine serum (FBS; Hyclone; GE Healthcare Life Sciences) for 72 h at 37˚C.…”

Section: Methodsmentioning

confidence: 99%

Three-dimensional simulated microgravity culture improves the proliferation and odontogenic differentiation of dental pulp stem cell in PLGA scaffolds implanted in mice

Pan

et al. 2016

Molecular Medicine Reports

View full text Add to dashboard Cite

Tooth regeneration through stem cell-based therapy is a promising treatment for tooth decay and loss. Human dental pulp stem cells (hDPSCs) have been widely identified as the stem cells with the most potential for tooth tissue regeneration. However, the culture of hDPSCs in vitro for tissue engineering is challenging, as cells may proliferate slowly or/and differentiate poorly in vivo. Dynamic three‑dimensional (3D) simulated microgravity (SMG) created using the rotary cell culture system is considered to an effective tool, which contributes to several cell functions. Thus, the present study aimed to investigate the effect of dynamic 3D SMG culture on the proliferation and odontogenic differentiation abilities of hDPSCs in poly (lactic‑co‑glycolic acid) (PLGA) scaffolds in nude mice. The hDPSCs on PLGA scaffolds were maintained separately in the 3D SMG culture system and static 3D cultures with osteogenic medium for 7 days in vitro. Subsequently, the cell‑PLGA complexes were implanted subcutaneously on the backs of nude mice for 4 weeks. The results of histological and immunohistochemical examinations of Ki‑67, type I collagen, dentin sialoprotein and DMP‑1 indicated that the proliferation and odontogenic differentiation abilities of the hDPSCs prepared in the 3D SMG culture system were higher, compared with those prepared in the static culture system. These findings suggested that dynamic 3D SMG culture likely contributes to tissue engineering by improving the proliferation and odontogenic differentiation abilities of hDPSCs in vivo.

show abstract

“…Dental pulp stem cells were isolated and cultured as previously described (He et al 2016). In brief, pulp tissue was obtained from healthy human third molars (patients aged 18-22 years) at the Hospital of Stomatology, Wuhan University.…”

Section: Cell Culturesmentioning

confidence: 99%

Vascular endothelial growth factor A/Vascular endothelial growth factor receptor 2 axis promotes human dental pulp stem cell migration via the FAK/PI3K/Akt and p38 MAPK signalling pathways

et al. 2019

View full text Add to dashboard Cite

Aim To investigate the effects of vascular endothelial growth factor A (VEGFA) and the underlying molecular mechanisms on the migration of human dental pulp stem cells (hDPSCs). Methodology The expression of VEGFA in inflammatory pulp tissue and lipopolysaccharide (LPS)‐stimulated dental pulp cells was examined by immunofluorescence staining and qRT‐PCR. The migration of hDPSCs was detected using transwell migration and wound healing assays. The activation of FAK, PI3K, Akt and p38 signalling was evaluated by Western blot analysis. Silence RNA (siRNA) technology was utilized to knockdown the expression of VEGFR1 (Flt‐1) and VEGFR2 (Flk‐1/KDR). PF573228 (inhibitor of FAK), LY294002 (inhibitor of PI3K), SB203580 (inhibitor of p38) and SU5416 (inhibitor of VEGFR2) were employed to investigate the effect of VEGFA on the migratory mechanism of hDPSCs. Data were analysed statistically using the Student’s t‐test or one‐way ANOVA. Results The expression levels of VEGFA in inflammatory pulp tissue in vivo and LPS‐stimulated dental pulp cells in vitro were significantly greater than those in the control groups (P < 0.05). Vascular endothelial growth factor A promoted the migration of hDPSCs in a concentration‐dependent manner. Several signalling pathways, including FAK, PI3K, Akt and p38, were activated by VEGFA in a dose‐ and time‐dependent manner in hDPSCs. The VEGFA‐induced migration of hDPSCs was significantly inhibited with drug inhibitors such as PF573228, LY294002, SB203580 or SU5416 (P < 0.05). These signalling pathways activated by VEGFA stimulation were significantly suppressed by pre‐treatment with inhibitor of VEGFR2 (SU5416) or transfection with siRNA of VRGFR2 (P < 0.05) but not VEGFR1 siRNA. Conclusions Vascular endothelial growth factor A/VEGFR2 axis promoted the migration of hDPSCs via the FAK/PI3K/Akt and p38 MAPK signalling pathways. These findings reveal a novel molecular mechanism for cell migration of hDPSCs, which may contribute to the remodelling of pulp tissue and dentine.

show abstract

Increased proliferation and adhesion properties of human dental pulp stem cells in PLGA scaffolds via simulated microgravity

Abstract: SMG may regulate the behaviour of hDPSCs grown in PLGA scaffolds in an integrin-mediated manner, which may contribute to tooth tissue engineering by increasing their expandability and scaffold adhesion.

Cited by 27 publications

References 49 publications

Thyroid cancer cells in space during the TEXUS-53 sounding rocket mission – The THYROID Project

Thyroid cancer cells in space during the TEXUS-53 sounding rocket mission – The THYROID Project

Three-dimensional simulated microgravity culture improves the proliferation and odontogenic differentiation of dental pulp stem cell in PLGA scaffolds implanted in mice

Vascular endothelial growth factor A/Vascular endothelial growth factor receptor 2 axis promotes human dental pulp stem cell migration via the FAK/PI3K/Akt and p38 MAPK signalling pathways

Contact Info

Product

Resources

About