Increased podocyturia in pregnant women compared to non‐pregnant women

Furuta, Itsuko; Zhai, Tianyue; Umazume, Takeshi; Ishikawa, Satoshi; Nakagawa, Kinuko; Akaishi, Rina; Yamada, Takahiro; Morikawa, Mamoru; Minakami, Hisanori

doi:10.1111/jog.13283

Cited by 2 publications

(2 citation statements)

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“…Nephrin mRNA expression was 2.87 copies/mg Cr (range, 0.12–31.7 copies/mg Cr) and 1.46 (range, 0.06–26.8 copies/mg Cr; P = 0.4104) before and after delivery, respectively. Post‐partum decrease in podocin mRNA expression in NCP women suggested increased podocyturia even in NCP pregnancies, confirming the results of a previous study …”

Section: Resultssupporting

confidence: 89%

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Post‐partum podocyturia following pre‐eclamptic pregnancy

Furuta

Zhai

Umazume

et al. 2017

J of Obstet and Gynaecol

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Section: Resultssupporting

confidence: 89%

“…Post-partum decrease in podocin mRNA expression in NCP women suggested increased podocyturia even in NCP pregnancies, confirming the results of a previous study. 15…”

Section: Changes In Parameters With Time After Childbirthmentioning

confidence: 99%

Post‐partum podocyturia following pre‐eclamptic pregnancy

Furuta

Zhai

Umazume

et al. 2017

J of Obstet and Gynaecol

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Urine-derived podocytes from steroid resistant nephrotic syndrome patients as a model for renal-progenitor derived extracellular vesicles effect and drug screening

Tanzi,

Buono,

Grange

et al. 2024

Preprint

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Background Personalized disease models are crucial for assessing the specific response of diseased cells to drugs, particularly novel biological therapeutics. Extracellular vesicles (EVs), nanosized vesicles released by cells for intercellular communication, have gained therapeutic interest due to their ability to reprogram target cells. We here utilized urinary podocytes obtained from children affected by steroid-resistant nephrotic syndrome with characterized genetic mutations as a model to test the therapeutic potential of EVs derived from kidney progenitor cells. Methods EVs were isolated from kidney progenitor cells (nKPCs) derived from the urine of a preterm neonate. Three lines of urinary podocytes obtained from nephrotic patients' urine and a line of Alport patient podocytes were characterized and used to assess albumin permeability in response to various drugs or to nKPC-EVs. RNA sequencing was conducted to identify commonly modulated pathways. Results Podocytes appeared unresponsive to pharmacological treatments, except for a podocyte line demonstrating responsiveness, in alignment with the patient's clinical response at 48 months. At variance, treatment with the nKPC-EVs was able to significantly reduce permeability in all the steroid-resistant patients-derived podocytes as well as in the line of Alport-derived podocytes. RNA sequencing of nKPC-EV-treated podocytes revealed the common upregulation of two genes (small ubiquitin-related modifier 1 (SUMO1) and Sentrin-specific protease 2 (SENP2)) involved in the SUMOylation pathway, a process recently demonstrated to play a role in slit diaphragm stabilization. Gene ontology analysis on podocyte expression profile highlighted cell-to-cell adhesion as the primary upregulated biological activity in treated podocytes. Conclusions nKPCs emerge as a promising non-invasive source of EVs with potential therapeutic effects on podocyte dysfunction. Furthermore, our findings suggest the possibility of establishing a non-invasive in vitro model for screening regenerative compounds on patient-derived podocytes.

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Increased podocyturia in pregnant women compared to non‐pregnant women

Abstract: Podocyturia monitored by Pod- and Nep-mRNA expression and urine cells expressing AQP2-mRNA were increased in uncomplicated pregnancies compared to healthy non-pregnant women. Urine cells expressing AQP2-mRNA increased with increasing podocyturia in healthy women.

Cited by 2 publications

References 27 publications

Post‐partum podocyturia following pre‐eclamptic pregnancy

Post‐partum podocyturia following pre‐eclamptic pregnancy

Urine-derived podocytes from steroid resistant nephrotic syndrome patients as a model for renal-progenitor derived extracellular vesicles effect and drug screening

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