Increased plasma membrane fluidity and decreased receptor availability of nonmuscle cells in myotonic dystrophy

Hübner, Christoph; Lindner, S G; Albani, M.; Ballmann, Manfred; Keup, B.; Schürmann, Melanie; Stegner, H.-E.; Claußen, Maike; Kohlschütter, Alfried

doi:10.1016/0022-510x(89)90137-8

Cited by 10 publications

(2 citation statements)

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“…Changes in the motional freedom of lipids and protein molecules in the cellular membrane represent a critical feature of pathological cells, influencing several aspects of their behaviour such as the response to chemotherapeutic agents [48], [49], availability or activation of membrane receptors [50], [51], migratory, invasive and/or metastatic potential [52], [53]. Even though no univocal trend is evident regarding the direction of alterations in tumor cell membrane fluidity [54], in most tumors malignant cell membranes have been found to possess higher fluidity than those of their healthy counterparts.…”

Section: Discussionmentioning

confidence: 99%

Epithelial-Stromal Interactions in Human Breast Cancer: Effects on Adhesion, Plasma Membrane Fluidity and Migration Speed and Directness

et al. 2012

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Interactions occurring between malignant cells and the stromal microenvironment heavily influence tumor progression. We investigated whether this cross-talk affects some molecular and functional aspects specifically correlated with the invasive phenotype of breast tumor cells (i.e. adhesion molecule expression, membrane fluidity, migration) by co-culturing mammary cancer cells exhibiting different degrees of metastatic potential (MDA-MB-231>MCF-7) with fibroblasts isolated from breast healthy skin (normal fibroblasts, NFs) or from breast tumor stroma (cancer-associated fibroblasts, CAFs) in 2D or 3D (nodules) cultures. Confocal immunofluorescence analysis of the epithelial adhesion molecule E-cadherin on frozen nodule sections demonstrated that NFs and CAFs, respectively, induced or inhibited its expression in MCF-7 cells. An increase in the mesenchymal adhesion protein N-cadherin was observed in CAFs, but not in NFs, as a result of the interaction with both kinds of cancer cells. CAFs, in turn, promoted N-cadherin up-regulation in MDA-MB-231 cells and its de novo expression in MCF-7 cells. Beyond promotion of “cadherin switching”, another sign of the CAF-triggered epithelial-mesenchymal transition (EMT) was the induction of vimentin expression in MCF-7 cells. Plasma membrane labeling of monolayer cultures with the fluorescent probe Laurdan showed an enhancement of the membrane fluidity in cancer cells co-cultured with NFs or CAFs. An increase in lipid packing density of fibroblast membranes was promoted by MCF-7 cells. Time-lapsed cell tracking analysis of mammary cancer cells co-cultured with NFs or CAFs revealed an enhancement of tumor cell migration velocity, even with a marked increase in the directness induced by CAFs.Our results demonstrate a reciprocal influence of mammary cancer and fibroblasts on various adhesiveness/invasiveness features. Notably, CAFs' ability to promote EMT, reduction of cell adhesion, increase in membrane fluidity, and migration velocity and directness in mammary cancer cells can be viewed as an overall progression- and invasion-promoting effect.

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Section: Discussionmentioning

confidence: 99%

Epithelial-Stromal Interactions in Human Breast Cancer: Effects on Adhesion, Plasma Membrane Fluidity and Migration Speed and Directness

et al. 2012

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“…Dextran sulfate downrcgulates Fc and CD lib / CD 18 receptor expression [5] and abrogates HIV ad sorption to CD4 receeptor-positive cells [6], Like wise, leukocyte/endothelium interaction has recently been found reduced by dextran sulfate and other sulfated polysaccharides [7], In order to investigate whether plasma membrane fluidization might be re sponsible for the downregulation of CD llb/CD 18 receptors and the reduced leukocyte adhesion, we measured the effect of dextran sulfate on plasma membrane fluidity of peripheral white blood cells, using steady-state fluorescence anisotropy of the classical membrane probe diphenylhexatriene [8], Anisotropy values in dextran sulfate-treated cells were decreased as compared with control cells, indi cating increased fluidity in the hydrophobic mem brane interior.…”

Section: Dm Saumweber R Bergmann C Hammermentioning

confidence: 99%