Increased plasma levels of lncRNA H19 and LIPCAR are associated with increased risk of coronary artery disease in a Chinese population

Abstract

Recent studies in animal models and humans show that long non-coding RNAs (lncRNAs) are involved in the development of atherosclerosis, which contributes to the pathological foundation of coronary artery disease (CAD). LncRNAs in plasma and serum have been considered as promising novel biomarkers for diagnosis and prognosis of cardiovascular diseases, especially CAD. We here measured the circulating levels of 8 individual lncRNAs which are known to be relevant to atherosclerosis in the plasma samples from 300 patients with CAD and 180 control subjects by using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) methods. We found that the plasma level of H19 and long intergenic non-coding RNA predicting cardiac remodeling (LIPCAR) were significantly increased in patients with CAD. The area under the receiver operating characteristic curve was 0.631 for H19 and 0.722 for LIPCAR. Multivariate logistic regression analyses indicated that plasma H19 and LIPCAR were independent predictors for CAD, even after adjustment for traditional cardiovascular risk factors. Further studies identified that plasma levels of H19 and LIPCAR were also increased in CAD patients with heart failure compared to those with normal cardiac function. Taken together, our results suggest that increased plasma levels of H19 and LIPCAR are associated with increased risk of CAD and may be considered as novel biomarkers for CAD.

Long Non-Coding RNAs Link Oxidized Low-Density Lipoprotein With the Inflammatory Response of Macrophages in Atherogenesis

“…Long non-coding RNA H19 was significantly upregulated in the serum of patients with atherosclerosis and those with a high risk of coronary artery disease in a Chinese population (125,126). In addition, H19 was also induced by ox-LDL and inhibition of H19 protected HUVECs and HAVSMCs against ox-LDL induced apoptosis via sponging miR-148b or miR-let-7, respectively (88,89).…”

MALAT1 promoted cell proliferation and migration via MALAT1/miR‐155/MEF2A pathway in hypoxia of cardiac stem cells

“…In recent decades, lncRNAs acquire recognition for their importance in a variety of biological processes, including growth, differentiation, pluripotency, embryonic development, homeostasis, and disease. 18 Furthermore, emerging evidence has suggested that lncRNAs played an important role in hypoxia via regulating hypoxic gene expression at transcriptional, and posttranscriptional, and translation levels. 14,15 For example, lncRNA BACE1-AS was upregulated in heart failure, 16 plasma lncRNA UCA1 expression was associated with poor prognosis in chronic heart failure patients 17 and lncRNA H19 expression was associated with increased risk of coronary artery disease.…”

Circulating lncRNA IFNG‐AS1 expression correlates with increased disease risk, higher disease severity and elevated inflammation in patients with coronary artery disease

“…A growing number of recent studies display that lncRNAs play pivotal roles in diverse physiological and pathological processes in the cardiovascular system. For example, increased plasma levels of lncRNA H19 and LIPCAR are associated with increased risk of CAD . A study reports that long intergenic noncoding RNA 00305 sponges miR‐136 to regulate the hypoxia‐induced apoptosis of human umbilical vein endothelial cells .…”