Increased plasma interleukin-8 concentrations in alcoholic hepatitis

Abstract

Patients with alcoholic hepatitis often have hepatic polymorphonuclear leukocyte infiltration and neutrophilia. Interleukin-8 is a cytokine that stimulates neutrophil chemotaxis and release of lysosomal enzymes. It is made by several types of cells, including fibroblasts, Kupffer cells and hepatocytes. In this study, serial plasma interleukin-8 concentrations were measured with enzyme-linked immunosorbent assay in 40 consecutive patients with moderate-to-severe alcoholic hepatitis over a 6-mo period. Two control groups included 10 patients without clinically important liver disease admitted for treatment of alcohol dependence and 12 healthy male volunteers. The mean plasma interleukin-8 level on admission was markedly increased: 695 +/- 146 pg/ml in the alcoholic hepatitis patients. The alcohol-dependent control group and the normal volunteer controls had mean interleukin-8 concentrations of 106 +/- 28 pg/ml and 10 +/- 5 pg/ml, respectively. Initially increased interleukin-8 levels in alcoholic hepatitis patients decreased to a mean of 182 +/- 42 pg/ml over the first month; levels had decreased further to 124 +/- 79 pg/ml after 6 mo. Increased interleukin-8 concentrations in patients with alcoholic hepatitis suggest a role for interleukin-8 in the neutrophilia and hepatic polymorphonuclear leukocyte infiltration of alcoholic hepatitis.

Interleukin-8 and hIRH (SDF1-?/PBSF) mRNA expression and histological activity index in patients with chronic hepatitis C

“…10 In certain pathological situations, IL-8 is produced excessively by a wide variety of cells and it is released in the circulation. Previous studies showed that IL-8 secretion or IL-8 gene expression in chronic or acute liver diseases such as cirrhosis and autoimmune or alcoholic hepatitis, were increased compared with normal controls, [13][14][15][16][17] but without attempts at defining which components of liver injury may be most affected.…”

“…11,12 Previous studies have shown that IL-8 secretion or IL-8 gene expression in chronic or acute liver disease, such as cirrhosis and alcoholic or autoimmune hepatitis, were increased compared with normal control groups. [13][14][15][16][17] However, the role of hIRH as well as IL-8 in the pathogenesis of hepatitis C virus (HCV)-related hepatitis remains unclear.…”

Activation of nuclear factor kappa B and cytokine imbalance in experimental alcoholic liver disease in the rat

“…13,14 There is also increasing support for a role for chemokines in alcoholic liver injury. 15,16 Chemokines are a group of cytokines that exhibit strong chemoattractant activities and are involved in inflammatory cell migration. 17,18 To evaluate the relationship between pathological liver injury and activation of NF-B and cytokine disregulation, we used the rat intragastric feeding model for alcoholic liver disease.…”

Chronic alcohol intoxication induces hepatic injury through enhanced macrophage inflammatory protein-2 production and intercellular adhesion molecule-1 expression in the liver

“…observed in patients with alcoholic liver disease. 3 The human equivalent of IL-8 has not been found in the rat, but an ILRecombinant rat MIP 2 (Biosource) has a molecular weight of 8 kd (73 amino acids) and stimulates neutrophil chemotaxis, 8-like molecule, most likely related to keratinocyte cytokine/ growth related cytokine has been detected. 6,9,10,27 Another superoxide release, and degranulation.…”

“…[1][2][3] In addition to IL-8 Orleans, LA. or IL-8-like chemokine, macrophage inflammatory proteins and purity was more than 97%, as assessed by Trypan blue exclusion are thought to contribute to hepatic injury in alcoholics and technique and peroxidase staining. endotoxemia, the mediators responsible for these manifestaIsolation of Hepatocytes and Nonparenchymal Cells.…”