Increased plasma complement factor H is associated with geriatric depression

Shin, Cheolmin; Ham, Byung Joo; Ko, Young Hoon; Pae, Chi Un; Park, Moon Ho; Steffens, David C.; Patkar, Ashwin A.

doi:10.1017/s1041610218000558

Cited by 18 publications

(19 citation statements)

References 32 publications

(35 reference statements)

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“…While this produced several data points for analysis, with samples incubated at four storage conditions and assayed in duplicate, a larger study is needed to confirm the effect of these sample treatments. We reviewed the five publications that also measured complement proteins using MILLIPLEX ® kits referenced by Sandholm et al [8][9][10][11][12] Two references used MILLIPLEX ® panels other than MILLIPLEX ® Human Complement Panel 2 for measurement of C4 (HNDG2MAG-36K) and C3 and Factor H (HNDG1MAG-36K). 8,9 The MILLIPLEX ® Human Complement Panel 2 was optimized to match available reference ranges for serum/plasma.…”

Section: Discussionmentioning

confidence: 99%

“…We reviewed the five publications that also measured complement proteins using MILLIPLEX ® kits referenced by Sandholm et al [8][9][10][11][12] Two references used MILLIPLEX ® panels other than MILLIPLEX ® Human Complement Panel 2 for measurement of C4 (HNDG2MAG-36K) and C3 and Factor H (HNDG1MAG-36K). 8,9 The MILLIPLEX ® Human Complement Panel 2 was optimized to match available reference ranges for serum/plasma. Some complement analytes are also present in other panels in the MILLIPLEX ® portfolio, and were developed using different reagents, and tested in conditions suitable for each panel.…”

Section: Discussionmentioning

confidence: 99%

“…We reviewed the five publications that also measured complement proteins using MILLIPLEX ® kits referenced by Sandholm et al 8‐12 Two references used MILLIPLEX ® panels other than MILLIPLEX ® Human Complement Panel 2 for measurement of C4 (HNDG2MAG‐36K) and C3 and Factor H (HNDG1MAG‐36K) 8,9 . The MILLIPLEX ® Human Complement Panel 2 was optimized to match available reference ranges for serum/plasma.…”

Section: Discussionmentioning

confidence: 99%

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Complement factor serum and plasma levels measured by a MILLIPLEX^® Magnetic Bead Panel: A reply

Keith¹,

Gilliam²,

Pépin³

et al. 2021

Scand J Immunol

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BACKGROUNDWe would like to thank you for the opportunity to respond to the discrepancies raised in Dr Sandholm's letter and to present our recent findings. Sandholm and colleagues previously described the rationale for testing patient samples for complement component levels for the study and treatment of numerous pathological conditions, as well as the advantages of multiplex assays over single enzyme immunoassays. 1 There is an expectation that commercially available immunoassay kits, including the research use only MILLIPLEX ® Human Complement Magnetic Bead Panel 2 (HCMP2MAG-19K), will perform accurately and consistently. These studies were initiated in response to the published results of Sandholm et al 1 to investigate the cause of aberrant sample values, particularly for C3. | MATERIALS AND METHODSWe tested the commercially available MILLIPLEX ® Magnetic Bead Panel, HCMP2MAG-19K (Merck, Darmstadt, Germany), which consists of a multiplexed immunoassay for complement factors C1q, C3, C3b/iC3b, C4, Factor B, H, and Properdin. This panel is for research use only, not for use in diagnostic procedures. Serum and plasma samples were tested at a 40 000-fold dilution. Samples were also tested with the MicroVue ™ Complement Factor H EIA (Quidel, San Diego, CA, USA).Blood samples included serum (n = 8) and K2EDTAplasma (n = 10) for which C3 values had been clinically determined using nephelometry (Discovery Life Sciences, Los Osos, CA, USA) and matched donor samples (n = 4) of serum, K2EDTA-plasma and lepirudin plasma (BioIVT, Westbury, NY, USA). Blood samples were stored at −80°C pending analysis, except during the heat-stress study described below. All assays were run according to the manufacturer's instructions.For the heat-stress study, four aliquots were taken from each sample type (serum, K2EDTA-plasma, lepirudin plasma) from each donor and incubated at 4°C for 24 hours, room temperature for 24 hours, or 37°C for 2 hours pending analysis. One aliquot was kept at −80°C as a control. While four donors are a small subset, the three sample types and four temperature conditions produced 96 data points with samples assayed in duplicate and were considered sufficient for the observation of any large deviation of sample values from acceptable C3 reference ranges as indicated by Sandholm et al.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Complement factor serum and plasma levels measured by a MILLIPLEX^® Magnetic Bead Panel: A reply

Keith¹,

Gilliam²,

Pépin³

et al. 2021

Scand J Immunol

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“…CFH is critical for activation of the alternate complement system. In a recent study, increased levels of CFH in plasma were positively correlated with depression in geriatric patients (Shin et al 2019). Genetic variants of the CFH gene, which encodes the CFH protein, are associated with age-related macular degeneration (AMD).…”

Section: Discussionmentioning

confidence: 99%

“…Complement factor H (CFH) is an important regulator of the alternative pathway of the complement system. Interestingly, increased plasma CFH has been found to be correlated with geriatric depression (Shin et al 2019). Additionally, genetic variants of CFH are strongly associated with age-related macular degeneration (AMD), and CFH has been shown to inhibit the anti-inflammatory activity of CD47 (Calippe et al 2017).…”

Section: Macrophage Stimulating 1 Decreases With Nt-020mentioning

confidence: 99%

Effects of nutraceutical intervention on serum proteins in aged rats

et al. 2020

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Aging is associated with many pathophysiological changes that could lead to the onset of degenerative disease. Some of the physiological changes that occur with aging include increased inflammation and decreased stem cell proliferation, leading to decreased capacity for tissue regeneration and loss of function. In previous studies, we and others have found nutraceutical intervention to ameliorate some of the deleterious effects associated with aging. In particular, we have previously shown that NT-020, a supplement composed of a proprietary blend of blueberries, green tea, vitamin D3, and carnosine, is able to rescue age-related cognitive deficits, impaired neurogenesis, and inflammation in rats. We have also previously demonstrated that stem cells cultured with old serum showed decreased proliferation; however, when stem cells were cultured in serum from old rats given a diet supplemented with NT-020, proliferation did not differ from that of cells cultured with serum from young rats. While it is clear that NT-020 is exerting a therapeutic, anti-aging effect, the mechanisms of action were yet to be fully elucidated.To that end, in the present study, we conducted a bioinformatics experiment to examine the rat proteome of serum from young and old control rats and young and old rats given a diet supplemented with NT-020. Serum from old rats showed an increase in some inflammatory and pro-aging factors while serum from old rats given a diet supplemented with NT-020 showed an increase in some anti-aging factors, most notably proteins associated with the complement system and autophagy. A number of immune functions that increase with age were shown to be downregulated with NT-020 treatment.

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Reduced plasma Fetuin-A is a promising biomarker of depression in the elderly

Fanelli

Benedetti

Wang

et al. 2019

Eur Arch Psychiatry Clin Neurosci

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Increased plasma complement factor H is associated with geriatric depression

Abstract: This study revealed an association between high plasma levels of CFH and geriatric depression, thereby suggesting the alternative pathway of the complement system contributing to the development of geriatric depression.

Cited by 18 publications

References 32 publications

Complement factor serum and plasma levels measured by a MILLIPLEX^® Magnetic Bead Panel: A reply

Complement factor serum and plasma levels measured by a MILLIPLEX^® Magnetic Bead Panel: A reply

Effects of nutraceutical intervention on serum proteins in aged rats

Reduced plasma Fetuin-A is a promising biomarker of depression in the elderly

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Increased plasma complement factor H is associated with geriatric depression

Abstract: This study revealed an association between high plasma levels of CFH and geriatric depression, thereby suggesting the alternative pathway of the complement system contributing to the development of geriatric depression.

Cited by 18 publications

References 32 publications

Complement factor serum and plasma levels measured by a MILLIPLEX® Magnetic Bead Panel: A reply

Complement factor serum and plasma levels measured by a MILLIPLEX® Magnetic Bead Panel: A reply

Effects of nutraceutical intervention on serum proteins in aged rats

Reduced plasma Fetuin-A is a promising biomarker of depression in the elderly

Contact Info

Product

Resources

About

Complement factor serum and plasma levels measured by a MILLIPLEX^® Magnetic Bead Panel: A reply

Complement factor serum and plasma levels measured by a MILLIPLEX^® Magnetic Bead Panel: A reply