Increased plasma 17-hydroxyprogesterone and milt production in response to gonadotropin-releasing hormone agonist in captive male starry flounder, Platichthys stellatus

Moon, Sungjin; Lim, Han-kyu; Kwon, Joon Yeong; Lee, J.K; Chang, Yen-Jui

doi:10.1016/s0044-8486(02)00643-9

Cited by 26 publications

(12 citation statements)

References 26 publications

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“…A multiple hCG injection protocol has not been assayed in flatfishes, but weekly injections of 1,500 UI kg -1 were highly effective in stimulating 11-KT secretion in the European eel (Anguilla anguilla) (Peñaranda et al, 2009). Stimulation of androgen release by GnRHa treatment has been previously reported in several flatfishes, such as the winter flounder (Pleuronectes americanus) (Harmin et al, 1995), plaice (Pleuronectes platessa) (Vermeirssen et al, 1998), starry flounder (Platichthys stellatus) (Moon et al, 2003) and the Senegalese sole (Agulleiro et al, 2006(Agulleiro et al, , 2007. Taken together, these and the present study confirm the stimulatory effect of GnRHa on 11-KT and T release, likely through the action of GnRHainduced pituitary secretion of endogenous gonadotropins (Yaron et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…In aquaculture, the most common strategy to stimulate gonad maturation, ovulation and spermiation in fish is the exogenous treatment with gonadotropin-releasing hormone agonists (GnRHa), either in the form of liquid injections or sustained-release delivery systems Mylonas and Zohar, 2001;Mylonas et al, 2009). The GnRHa based hormonal treatments have been successfully used in several flatfish species to induce spawning and spermiation, normally with highest efficiency exhibited by treatments using GnRHa slow-release delivery implants (Larsson et al, 1997;Mugnier et al, 2000;Vermeirssen et al, 2000;Moon et al, 2003). Although less widely used than GnRHa, another effective hormonal therapy is the treatment with human chorionic gonadotropin (hCG), usually administered through saline-diluted multiple injections.…”

Section: Introductionmentioning

confidence: 99%

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Comparative effects of human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone agonist (GnRHa) treatments on the stimulation of male Senegalese sole (Solea senegalensis) reproduction

et al. 2011

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The aquaculture of Senegalese sole (Solea senegalensis) is limited by the failure of cultured breeders (G1 generation) to produce fertilized spawning. Critical reproductive dysfunctions have been observed in both female and male Senegalese sole cultured breeders, including reduced fecundity and diminished sperm production. The present work aimed to study the effectiveness of different hormonal treatments on the stimulation of male reproduction. Male Senegalese sole cultured breeders were treated with 1) saline injections (controls), 2) gonadotropin-releasing hormone agonist ). Each group of males was placed in separated spawning tanks together with females treated with GnRHa implants.All three hormonal treatments increased plasma testosterone (T) and 11-ketotestosterone (11-KT) levels and the gonadosomatic index (GSI), with highest effects exerted by the hCG treatment. Histological examination of the testes showed no effect of the GnRHa injection, but a clear stimulation of germ cell proliferation and testicular maturation by GnRHa implants and hCG injections. As expected, GnRHa implantation of females induced egg release in all experimental tanks and interestingly, female fecundity increased in tanks containing GnRHa-or hCG-treated males. A fertilized spawning was obtained only from the group containing hCG-treated males. In conclusion, hormonal treatments stimulated steroidogenesis and spermatogenesis in male Senegalese sole, with highest efficiency of the hCG multiple injection treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative effects of human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone agonist (GnRHa) treatments on the stimulation of male Senegalese sole (Solea senegalensis) reproduction

et al. 2011

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“…GnRHa and hCG treatment has been tested successfully in starry flounder males and has been shown to be an effective strategy for enhancing milt quantity The superscripts indicate significant differences between treatments at that sampling time (P \ 0.05). The arrow indicates the timing of the second injection Different superscripts indicate significant differences between treatments (P \ 0.05) (Lim et al 2002;Moon et al 2003;. hCG and GnRHa, administered short or long term via injection or pellet implant, increased the milt volume in previous experiments with starry flounder (Lim et al 2002;Moon et al 2003;.…”

Section: Discussionmentioning

confidence: 93%

“…The arrow indicates the timing of the second injection Different superscripts indicate significant differences between treatments (P \ 0.05) (Lim et al 2002;Moon et al 2003;. hCG and GnRHa, administered short or long term via injection or pellet implant, increased the milt volume in previous experiments with starry flounder (Lim et al 2002;Moon et al 2003;. However, the effect of exogenous hormones on starry flounder ovulation is unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, it is important to characterize the response to these treatments. Protocol development for brood stock management has included studies of the effects of GnRHa on the stimulation of milt production and spermiation in male starry flounder (Lim et al 2002;Moon et al 2003;. Treatment of male starry flounder with GnRHa increased the milt volume, confirming the role of GtH in stimulating milt volume increase in this species over long periods.…”

Section: Introductionmentioning

confidence: 96%

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Effect of exogenous hormones on ovulation and gonadal steroid plasma levels in starry flounder, Platichthys stellatus

Lim

2016

Aquacult Int

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The present study was designed to examine the potential for inducing ovulation in starry flounder (Platichthys stellatus) using gonadotropin-releasing hormone analog (GnRHa) and human chorionic gonadotropin (hCG) to assess whether starry flounder are differentially responsive to GnRHa and hCG. Female starry flounder were injected or implanted with different doses of hCG or GnRHa pellets to examine their ovulationinducing potential and effects on plasma levels of testosterone (T), 17b-estradiol (E2), and 17,20b-dihydroxy-4-pregnen-3-one (17,20bP). Blood samples were collected for up to 10 or 25 days post-injection or post-implantation in two separate experiments designed to mimic the early and middle stages of spawning, respectively. Fish treated with the GnRHa pellets (100 lg) showed a significant increase in the total number of stripped eggs relative to the controls. GnRHa administration had no effect on the floating rate or fertilization rate of ovulated eggs in the both experiments, whereas hCG injection affected both of these rates. Plasma T levels were not significantly different between the exogenous hormonetreated and control fish. In contrast, the plasma E2 level was elevated in those fish treated with GnRHa, regardless of injection or implantation, and was accompanied by increased numbers of stripped eggs in both experiments. Treatment with GnRHa resulted in higher 17,20bP levels compared to the controls, and there was a positive relationship between elevated plasma 17,20bP and an increase in ovulated eggs in response to GnRHa treatment. The implantation of starry flounder with GnRHa-containing pellets was effective at inducing sustained ovulation compared to hCG treatment.

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The Role of Reproductive Sciences in the Preservation and Breeding of Commercial and Threatened Teleost Fishes

Mayer

2019

Advances in Experimental Medicine and Biology

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Increased plasma 17-hydroxyprogesterone and milt production in response to gonadotropin-releasing hormone agonist in captive male starry flounder, Platichthys stellatus

Cited by 26 publications

References 26 publications

Comparative effects of human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone agonist (GnRHa) treatments on the stimulation of male Senegalese sole (Solea senegalensis) reproduction

Comparative effects of human chorionic gonadotropin (hCG) and gonadotropin-releasing hormone agonist (GnRHa) treatments on the stimulation of male Senegalese sole (Solea senegalensis) reproduction

Effect of exogenous hormones on ovulation and gonadal steroid plasma levels in starry flounder, Platichthys stellatus

The Role of Reproductive Sciences in the Preservation and Breeding of Commercial and Threatened Teleost Fishes

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