Increased peroxisome proliferation is associated with early yeast replicative ageing

Deb, Rachayeeta; Ghose, Suchetana; Nagotu, Shirisha

doi:10.1007/s00294-022-01233-3

“…Peroxisomes influence longevity [ 59 ], and longevity increases the lifespan of stressed cells [ 60 ]. The CTA1 gene acts on EtOH tolerance and seems to degrade ROS [ 61 , 62 ]. We found that the CTA1 gene transmits MAPK signals to the longevity and peroxisomal pathways.…”

Section: Discussionmentioning

confidence: 99%

Integrative Analysis of the Ethanol Tolerance of Saccharomyces cerevisiae

Wolf

¹

,

Marques

²

,

Almeida

³

et al. 2023

IJMS

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Ethanol (EtOH) alters many cellular processes in yeast. An integrated view of different EtOH-tolerant phenotypes and their long noncoding RNAs (lncRNAs) is not yet available. Here, large-scale data integration showed the core EtOH-responsive pathways, lncRNAs, and triggers of higher (HT) and lower (LT) EtOH-tolerant phenotypes. LncRNAs act in a strain-specific manner in the EtOH stress response. Network and omics analyses revealed that cells prepare for stress relief by favoring activation of life-essential systems. Therefore, longevity, peroxisomal, energy, lipid, and RNA/protein metabolisms are the core processes that drive EtOH tolerance. By integrating omics, network analysis, and several other experiments, we showed how the HT and LT phenotypes may arise: (1) the divergence occurs after cell signaling reaches the longevity and peroxisomal pathways, with CTA1 and ROS playing key roles; (2) signals reaching essential ribosomal and RNA pathways via SUI2 enhance the divergence; (3) specific lipid metabolism pathways also act on phenotype-specific profiles; (4) HTs take greater advantage of degradation and membraneless structures to cope with EtOH stress; and (5) our EtOH stress-buffering model suggests that diauxic shift drives EtOH buffering through an energy burst, mainly in HTs. Finally, critical genes, pathways, and the first models including lncRNAs to describe nuances of EtOH tolerance are reported here.

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“…Interestingly, cells incompetent for β-oxidation have a less severe aging phenotype than cells devoid of peroxisomes, indicating that other peroxisomal functions are important in regulating CLS ( Lefevre et al 2013 ). Peroxisome proliferation is also associated with the early stage of replicative aging ( Deb et al 2022 ). Free oxidative radicals have long been suggested to regulate the aging process ( Harman 1972 ).…”

Section: Discussionmentioning

confidence: 99%

Tra1 controls the transcriptional landscape of the aging cell

Bari

¹

,

Berg

²

,

Genereaux

³

et al. 2022

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Gene expression undergoes considerable changes during the aging process. The mechanisms regulating the transcriptional response to cellular aging remain poorly understood. Here, we employ the budding yeast Saccharomyces cerevisiae to better understand how organisms adapt their transcriptome to promote longevity. Chronological lifespan (CLS) assays in yeast measure the survival of non-dividing cells at stationary phase over time, providing insights into the aging process of post-mitotic cells. Tra1 is an essential component of both the yeast SAGA/SLIK and NuA4 complexes, where it recruits these complexes to acetylate histones at targeted promoters. Importantly, Tra1 regulates the transcriptional response to multiple stresses. To evaluate the role of Tra1 in chronological aging, we took advantage of a previously characterized mutant allele that carries mutations in the TRA1 PI3K domain (tra1Q3). We found that loss of functions associated with tra1Q3 sensitized cells to growth media acidification and shortens lifespan. Transcriptional profiling reveals that genes differentially regulated by Tra1 during the aging process are enriched for components of the response to stress. Notably, expression of catalases (CTA1, CTT1) involved in hydrogen peroxide detoxification decreases in chronologically aged tra1Q3 cells. Consequently, they display increased sensitivity to oxidative stress. tra1Q3 cells are unable to grow on glycerol indicating a defect in mitochondria function. Aged tra1Q3 cells also display reduced expression of peroxisomal genes, exhibit decreased numbers of peroxisomes and cannot grow on media containing oleate. Thus, Tra1 emerges as an important regulator of longevity in yeast via multiple mechanisms.

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“…2013). Peroxisome proliferation is also associated with the early stage of replicative aging (Deb et al . 2022).…”

Section: Discussionmentioning

confidence: 99%

Tra1 controls the transcriptional landscape of the aging cell

Bari

¹

,

Berg

²

,

Genereaux

³

et al. 2022

Preprint

0

View full text Add to dashboard Cite

Gene expression undergoes considerable changes during the aging process. The mechanisms regulating the transcriptional response to cellular aging remain poorly understood. Here, we employ the budding yeast Saccharomyces cerevisiae to better understand how organisms adapt their transcriptome to promote longevity. Chronological lifespan (CLS) assays in yeast measure the survival of non-dividing cells at stationary phase over time, providing insights into the aging process of post-mitotic cells. Tra1 is an essential component of both the yeast SAGA/SLIK and NuA4 complexes, where it recruits these complexes to acetylate histones at targeted promoters. Importantly, Tra1 regulates the transcriptional response to multiple stresses. To evaluate the role of Tra1 in chronological aging, we took advantage of a previously characterized mutant allele that carries mutations in the TRA1 PI3K domain (tra1Q3). We found that loss of functions associated with tra1Q3 sensitized cells to growth media acidification and shortens lifespan. Transcriptional profiling reveals that genes differentially regulated by Tra1 during the aging process are enriched for components of the response to stress. Notably, expression of catalases (CTA1, CTT1) involved in hydrogen peroxide detoxification decreases in chronologically aged tra1Q3 cells. Consequently, they display increased sensitivity to oxidative stress. tra1Q3 cells are unable to grow on glycerol indicating a defect in mitochondria function. Aged tra1Q3 cells also display reduced expression of peroxisomal genes, exhibit decreased numbers of peroxisomes and cannot grow on media containing oleate. Thus, Tra1 emerges as an important regulator of longevity in yeast via multiple mechanisms.

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Increased peroxisome proliferation is associated with early yeast replicative ageing

Cited by 7 publications

References 75 publications

Integrative Analysis of the Ethanol Tolerance of Saccharomyces cerevisiae

Integrative Analysis of the Ethanol Tolerance of Saccharomyces cerevisiae

Tra1 controls the transcriptional landscape of the aging cell

Tra1 controls the transcriptional landscape of the aging cell

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