Increased Osteoid Formation in BMP-2–Loaded Silk-Based Screws

Abstract

The BMP-2-loaded silk-based fixation device in this study exhibited characteristics comparable to the current nonloaded silk screws with regard to integration and biocompatibility. However, functionalization of silk screws with BMP-2 appeared to allow for more organized collagen and osteoid deposition after 3 and 6 months and may increase the potential of successful remodeling.

Silk Biomaterials-Mediated miRNA Functionalized Orthopedic Devices

“…Previously, we demonstrated the capacity to load silkbased orthopedic devices with the antimicrobial drug Ciprofloxacin, 2 the osteoinductive growth factor bone morphogenic protein 2 (BMP2), 1,2 as well as the BMP2's peptide P24. 2 The study demonstrated the capacity to load different bioactive molecules, as well as the capacity for these molecules to maintain functionality.…”

“…O rthopedic silk medical devices, prepared from silkworm silk derived from Bombyx mori, represent new and promising candidates for bone repair and tissue regeneration due to their mechanical properties, low immunological responses, biodegradability, and ease of functionalization. [1][2][3][4][5][6] For bone fracture fixation, as well as guided tissue formation, successful engineered biomaterials must be biocompatible and resorbable, present an environment with appropriate cues for cell differentiation and deposition of new matrix, provide appropriate mechanical properties, and exhibit osteoconductivity. To this end, we recently developed a variety of chemical, structural, and biomechanical modifications of silk to improve function for bone tissue fracture repairs.…”

“…To this end, we recently developed a variety of chemical, structural, and biomechanical modifications of silk to improve function for bone tissue fracture repairs. 1,2,[4][5][6] These studies have focused on improving osteoconductivity through modifications with bioactive molecules to facilitate bone formation and for mechanical reinforcement.…”

“…List of Silk Biomaterials Used The thin wafer was attached to the base of the mold by melting both edges of the wax and then allowing it to dry. Silk plate blank molds were prepared using wax that was machined into a rectangular mold with inner dimensions of1.75 • 1.75 • 1.3 in 3 (4.45 • 4.45 • 3.…”

Silk Hydrogel-Mediated Delivery of Bone Morphogenetic Protein 7 Directly to Subcutaneous White Adipose Tissue Increases Browning and Energy Expenditure

“…Bioengineered silk protein delivery vehicles are an appealing candidate for mediating BMP7 delivery to WAT, as silk is a readily available natural resource (such as from silkworm production), is non-immunogenic, and is able to be engineered into microspheres, hydrogels and other drug delivery systems that protect the ‘drug’ from degradation, but are able to be regulated to control drug release kinetics (( Guziewicz et al, 2011 ; Hines and Kaplan, 2011 ; Pritchard et al, 2013a ; Pritchard et al, 2013b ; Guziewicz et al, 2013 ; Coburn et al, 2015 ; Lovett et al, 2015 ; Kluge et al, 2016 ; Zhang et al, 2017 )). Silk-mediated delivery of BMP2 has already been optimized in vitro and in mouse models ( Diab et al, 2012 ; Shi et al, 2013 ; Zhang et al, 2014 ; Koolen et al, 2016 ; Shen et al, 2016 ).…”