Increased nutritive value of transgenic potato by expressing a nonallergenic seed albumin gene from Amaranthus hypochondriacus

Chakraborty, Subhra; Chakraborty, Niranjan; Datta, Asis

doi:10.1073/pnas.050012697

Cited by 131 publications

(79 citation statements)

References 26 publications

(13 reference statements)

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“…Promoters of genes controlling starch and glycoprotein storage in the tubers of potato, cassava, sweet potato, taro or yam are widely used to improve their nutritional value or fight viral and fungal infections (Chakraborty et al 2000;Mohammed et al 2000). Several regulatory elements, such as dioscorin promoter pDJ3S, patatin class I promoters (B33 and PAT1), sporamin promoter and ß-amylase promoters are tuber-specific.…”

Section: Time-specific Promotersmentioning

confidence: 99%

Cis-regulatory elements used to control gene expression in plants

Biłas

Szafran

Hnatuszko-Konka

et al. 2016

Plant Cell Tiss Organ Cult

202

113

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Section: Time-specific Promotersmentioning

confidence: 99%

Cis-regulatory elements used to control gene expression in plants

Biłas

Szafran

Hnatuszko-Konka

et al. 2016

Plant Cell Tiss Organ Cult

202

113

View full text Add to dashboard Cite

“…Protein deficiency lowers resistance to disease, delays physical growth and development, and may cause permanent impairment of the brain in infants and young children. A major effort has been to improve the amino acid composition of plant protein because animals, including humans, are incapable of synthesizing 10 of the 21 amino acids required for protein synthesis, and these "essential amino acids" must therefore be obtained from the diet (1). Because of the importance of dietary protein and the fact that plants are its major source, development of strategies to increase protein levels and the concentration of essential amino acids in food crops is of primary importance in a crop improvement program.…”

mentioning

confidence: 99%

Next-generation protein-rich potato expressing the seed protein gene AmA1 is a result of proteome rebalancing in transgenic tuber

Chakraborty

Agrawal

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Protein deficiency is the most crucial factor that affects physical growth and development and that increases morbidity and mortality especially in developing countries. Efforts have been made to improve protein quality and quantity in crop plants but with limited success. Here, we report the development of transgenic potatoes with enhanced nutritive value by tuber-specific expression of a seed protein, AmA1 (Amaranth Albumin 1), in seven genotypic backgrounds suitable for cultivation in different agro-climatic regions. Analyses of the transgenic tubers revealed up to 60% increase in total protein content. In addition, the concentrations of several essential amino acids were increased significantly in transgenic tubers, which are otherwise limited in potato. Moreover, the transgenics also exhibited enhanced photosynthetic activity with a concomitant increase in total biomass. These results are striking because this genetic manipulation also resulted in a moderate increase in tuber yield. The comparative protein profiling suggests that the proteome rebalancing might cause increased protein content in transgenic tubers. Furthermore, the data on field performance and safety evaluation indicate that the transgenic potatoes are suitable for commercial cultivation. In vitro and in vivo studies on experimental animals demonstrate that the transgenic tubers are also safe for human consumption. Altogether, these results emphasize that the expression of AmA1 is a potential strategy for the nutritional improvement of food crops.allergenecity | essential amino acids | nutritional health

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“…Compared with traditional breeding strategies, the application of biotechnology to improve phytonutrient levels in whole foods is more difficult due to the complex array of potentially important chemicals and the complexity of the underlying biosynthetic pathways (Forkmann, 1991). Subhra et al, (2000). Improvement of nutritive value of crop plants, in particular the amino acid composition, has been a major long-term goal of plant breeding programs.…”

Section: Nutritional Componentsmentioning

confidence: 99%

Biotechnological Tools to Improve Quality Parameters in Temperate Vegetables

Srinivas¹,

Reddy²,

Saidaiah³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Biotechnology has played an important role towards the attainment of production sustainability of crops by using various biotechnological tools. Biotechnology has the potential to provide new opportunities for achieving enhanced crop productivity in a way that will alleviate poverty, improve food security and nutrition, and promote sustainable use of natural resources. Efforts are being made towards nutritional improvement of crops by using the tools of biotechnology by increasing the levels of essential nutrients. A major challenge of sustainable livelihood in the developing nations can be met with the judicious and rational application of biotechnology along with other equally powerful crop production systems. Adoption of biotechnology in agriculture has been the focus of controversy due to varied reasons, as questions have arisen regarding food and environmental safety. Compared with traditional breeding strategies, the application of biotechnology to improve phytonutrient levels in whole foods is more difficult due to the complex array of potentially important chemicals and the complexity of the underlying biosynthetic pathways. To genetic modifications to reduce browning and oxidation and extend the shelf life of prepackaged lettuce. It is a specific plant-produced substances (phytochemicals), as well as classes of phytochemicals that offer specific health benefits. Phytochemical families with clearly beneficial health properties include glucosinolates found in the brassica vegetables including broccoli; carotenoids, anthocyanins and flavonols found in many temperate vegetables. It is also exploring approaches to retard wilting and yellowing in leafy green vegetables. The targeted expression of cytokinins to senescing leaves has shown promise as a route to extending shelf life of leafy vegetables. In addition, most of the transformants with higher zeaxanthin levels showed also increased total carotenoid contents (up to 5.7-fold) and some of them exhibited reduced amounts of lutein. The increase in total carotenoids suggests that the genetic modification affects the regulation of the whole carotenoid biosynthetic pathway in potato tubers. Purple colour potato was developed by expressing anthocyanin pigment by a gene encoding MYB transcription factor. Transgenic potato was developed by expression of 1-SST (Sucrose: sucrose 1-fructosyl transferase) and 1-FFT (fructan: fructan1-fructosyl transferase) Gene from globe artichoke for inulin production.

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Increased nutritive value of transgenic potato by expressing a nonallergenic seed albumin gene from Amaranthus hypochondriacus

Cited by 131 publications

References 26 publications

Cis-regulatory elements used to control gene expression in plants

Cis-regulatory elements used to control gene expression in plants

Next-generation protein-rich potato expressing the seed protein gene AmA1 is a result of proteome rebalancing in transgenic tuber

Biotechnological Tools to Improve Quality Parameters in Temperate Vegetables

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