Increased number of Arginine-based salt bridges contributes to the thermotolerance of thermotolerant acetic acid bacteria, Acetobacter tropicalis SKU1100

Matsutani, Minenosuke; Hirakawa, Hideki; Nishikura, Mitsuteru; Soemphol, Wichai; Ali, Ibnaof Ali Ibnaof; Yakushi, Toshiharu; Matsushita, Kazunobu

doi:10.1016/j.bbrc.2011.04.126

Cited by 23 publications

(8 citation statements)

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“…This is the first report of an endopolygalacturonase gene in an A. pasteurianus strain. Indeed, the closest relative possessing such a gene is A. tropicalis NBRC 101654 [2]; A. tropicalis has been isolated from spontaneous cocoa bean fermentation processes as well [32]. Furthermore, a PCR assay indicated that this polygalacturonase gene was not widespread amongst A. pasteurianus strains isolated from spontaneous cocoa bean fermentations (Additional file 4).…”

Section: Resultsmentioning

confidence: 99%

“…AAB can be found on (tropical) fruits and flowers [2-4], in fermented foods [1,3], and as members of the Drosophila gut [5]. Overall, AAB are of industrial interest because of their physiology, which is the case for acetic acid production out of ethanol during vinegar, kombucha, or cocoa bean fermentation [6-8] as well as for fine chemical productions such as those of ascorbic acid and cellulose [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…However, draft genomes are available for A. pasteurianus 3P3 (originating from submerged wine vinegar) [21], A. pasteurianus NBRC 101655 (Thai pineapple) [22], A. pasteurianus subsp. pasteurianus LMG 1262 T (Dutch beer, type strain) [21], A. aceti NBRC 14818 (ethanol-based vinegar) [23], A. pomorum DM001 ( Drosophila gut) [5], and A. tropicalis NBRC 101654 (Thai fruit) [2,24]. In general, Acetobacter species possess relatively small genomes (approximately 3 Mb), including plasmids in particular cases [20,25-27].…”

Section: Introductionmentioning

confidence: 99%

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Complete genome sequence and comparative analysis of Acetobacter pasteurianus 386B, a strain well-adapted to the cocoa bean fermentation ecosystem

2013

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BackgroundAcetobacter pasteurianus 386B, an acetic acid bacterium originating from a spontaneous cocoa bean heap fermentation, proved to be an ideal functional starter culture for coca bean fermentations. It is able to dominate the fermentation process, thereby resisting high acetic acid concentrations and temperatures. However, the molecular mechanisms underlying its metabolic capabilities and niche adaptations are unknown. In this study, whole-genome sequencing and comparative genome analysis was used to investigate this strain’s mechanisms to dominate the cocoa bean fermentation process.ResultsThe genome sequence of A. pasteurianus 386B is composed of a 2.8-Mb chromosome and seven plasmids. The annotation of 2875 protein-coding sequences revealed important characteristics, including several metabolic pathways, the occurrence of strain-specific genes such as an endopolygalacturonase, and the presence of mechanisms involved in tolerance towards various stress conditions. Furthermore, the low number of transposases in the genome and the absence of complete phage genomes indicate that this strain might be more genetically stable compared with other A. pasteurianus strains, which is an important advantage for the use of this strain as a functional starter culture. Comparative genome analysis with other members of the Acetobacteraceae confirmed the functional properties of A. pasteurianus 386B, such as its thermotolerant nature and unique genetic composition.ConclusionsGenome analysis of A. pasteurianus 386B provided detailed insights into the underlying mechanisms of its metabolic features, niche adaptations, and tolerance towards stress conditions. Combination of these data with previous experimental knowledge enabled an integrated, global overview of the functional characteristics of this strain. This knowledge will enable improved fermentation strategies and selection of appropriate acetic acid bacteria strains as functional starter culture for cocoa bean fermentation processes.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Complete genome sequence and comparative analysis of Acetobacter pasteurianus 386B, a strain well-adapted to the cocoa bean fermentation ecosystem

2013

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“…pomorum DM001PRJNA65823/PRJNA60787Shin et al (2011) Drosophila melanogaster Ac. tropicalis NBRC 101654PRJNA68643/PRJDA46891Matsutani et al (2011)Fruits Asaia platycodi SF2.1CBLX010000001:27This study Anopheles stephensi Commensalibacter intestini A911PRJNA75109/PRJNA73359Roh et al (2008) Drosophila melanogaster Gluconacetobacter diazotrophicus PAl 5PRJNA61587/PRJNA377Bertalan et al (2009)Sugarcane plants Glucona. europaeus LMG 18494PRJNA73763/PRJEA61325Andrés-Barrao et al (2011)Reference strain Glucona.…”

Section: Methodsmentioning

confidence: 99%

Acetic Acid Bacteria Genomes Reveal Functional Traits for Adaptation to Life in Insect Guts

Chouaia

Gaiarsa

Crotti

et al. 2014

Genome Biology and Evolution

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Acetic acid bacteria (AAB) live in sugar rich environments, including food matrices, plant tissues, and the gut of sugar-feeding insects. By comparing the newly sequenced genomes of Asaia platycodi and Saccharibacter sp., symbionts of Anopheles stephensi and Apis mellifera, respectively, with those of 14 other AAB, we provide a genomic view of the evolutionary pattern of this bacterial group and clues on traits that explain the success of AAB as insect symbionts. A specific pre-adaptive trait, cytochrome bo3 ubiquinol oxidase, appears ancestral in AAB and shows a phylogeny that is congruent with that of the genomes. The functional properties of this terminal oxidase might have allowed AAB to adapt to the diverse oxygen levels of arthropod guts.

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“…In a previous study, we examined the mechanism underlying this thermotolerant phenotype by randomly knocking out the genes of thermotolerant Acetobacter tropicalis SKU1100 by transposon mutagenesis, and obtained several mutant strains exhibiting severe growth defects at higher growth temperature . Based on these thermosensitive transposon mutants and their draft genome sequence information (Matsutani et al, 2011), we have identified 24 genes possibly involved in the thermotolerance mechanism of A. tropicalis, which can be classified into several categories such as stress response, cell division, cell wall and membrane biosynthesis, and membrane transport. ATPR 0071, one of the 24 genes important for growth at high temperatures in A. tropicalis SKU1100, is annotated as a Na + /H + antiporter.…”

Section: Introductionmentioning

confidence: 99%

A novel Na+(K+)/H+ antiporter plays an important role in the growth of Acetobacter tropicalis SKU1100 at high temperatures via regulation of cation and pH homeostasis

Soemphol

Tatsuno

Okada

et al. 2015

Journal of Biotechnology

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A gene encoding a putative Na(+)/H(+) antiporter was previously proposed to be involved in the thermotolerance mechanism of Acetobacter tropicalis SKU 1100. The results of this study show that disruption of this antiporter gene impaired growth at high temperatures with an external pH>6.5. The growth impairment at high temperatures was much more severe in the absence of Na(+) (with only the presence of K(+)); under these conditions, cells failed to grow even at 30°C and neutral to alkaline pH values, suggesting that this protein is also important for K(+) tolerance. Functional analysis with inside-out membrane vesicles from wild type and mutant strains indicated that the antiporter, At-NhaK2 operates as an alkali cation/proton antiporter for ions such as Na(+), K(+), Li(+), and Rb(+) at acidic to neutral pH values (6.5-7.5). The membrane vesicles were also shown to contain a distinct pH-dependent Na(+)(specific)/H(+) antiporter(s) that might function at alkaline pH values. In addition, phylogenetic analysis showed that At-NhaK2 is a novel type of Na(+)/H(+) antiporter belonging to a phylogenetically distinct new clade. These data demonstrate that At-NhaK2 functions as a Na(+)(K(+))/H(+) antiporter and is essential for K(+) and pH homeostasis during the growth of A. tropicalis SKU1100, especially at higher temperatures.

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Increased number of Arginine-based salt bridges contributes to the thermotolerance of thermotolerant acetic acid bacteria, Acetobacter tropicalis SKU1100

Cited by 23 publications

References 34 publications

Complete genome sequence and comparative analysis of Acetobacter pasteurianus 386B, a strain well-adapted to the cocoa bean fermentation ecosystem

Complete genome sequence and comparative analysis of Acetobacter pasteurianus 386B, a strain well-adapted to the cocoa bean fermentation ecosystem

Acetic Acid Bacteria Genomes Reveal Functional Traits for Adaptation to Life in Insect Guts

A novel Na+(K+)/H+ antiporter plays an important role in the growth of Acetobacter tropicalis SKU1100 at high temperatures via regulation of cation and pH homeostasis

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