Increased neutrophilia in nasal polyps reduces the response to oral corticosteroid therapy

Wen, Wang; Liu, Wenlong; Zhang, Luo; Bai, Jing; Fan, Yunping; Xia, Wenfei; Luo, Qing; Zheng, Jun; Wang, Hongtian; Li, Zuwang; Xia, Jiahong; Jiang, Hongyan; Liu, Zheng; Shi, Jianbo; Li, Hua-Bin; Xu, Geng

doi:10.1016/j.jaci.2012.01.079

Cited by 249 publications

(277 citation statements)

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“…The treatment modalities for eosinophilic or neutrophilic infiltration of NP differ from each other. Studies showed corticosteroid is effective to eosinophilic CRS by improving patients' symptom scores and polyp size scores, reducing numbers of eosinophil in NP but not numbers of neutrophils or levels of their mediators (Sakuma et al 2011;Wen et al 2012). On the other hand, macrolides effectively reduced cytokines produced by inflammatory cells in nasal mucosa (Bernstein et al 1997).…”

Section: Discussionmentioning

confidence: 99%

“…Most histological studies of nasal polyp have been performed in Caucasian patients and data from Asian population is still lacking. The majority was found to be eosinophil-predominant, comprising 73-92.5% (Armengot et al 2010; interleukin-8, which is an important chemo-attractant of neutrophil, and size of nasal polyps (Wen et al 2012;Majima 2004), while it showed poor therapeutic efficacy and worse clinical response in eosinophilic NP (Haruna et al 2009;Peric et al 2011). By understanding the histological types of the NP, it would provide us certain prognostic information and allow specific tailored treatments.…”

Section: Discussionmentioning

confidence: 99%

“…All slides were examined under light microscope with 400x magnification and a total of 10 high power fields were chosen for inflammatory cell counting. The number of neutrophils and/or eosinophils was counted, and average number of inflammatory cells for each high power field was calculated (Wen et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Malaysian Nasal Polyps: Eosinophil or Neutrophil-Predominant

Husain¹

2016

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ABSTRAK Polip hidung jenis eosinofil biasa terjadi dalam populasi ABSTRACTEosinophil-type nasal polyp (NP) is common in Western population. This aim of this study was to determine the histology type of NP among different Malaysian ethnic groups. A total of 122 chronic rhinosinusitis with nasal polyposis (CRSwNP) patients were retrospectively enrolled and demographic data was recorded. The histological slides were retrieved. The number of eosinophils and non-eosinophils were counted and average number of inflammatory cells for each high power field was calculated. Eosinophil-predominant was seen in 32.8% of patients and 67.2% was non-eosinophil-predominant. Phenotypes of NP significantly showed 57

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Malaysian Nasal Polyps: Eosinophil or Neutrophil-Predominant

Husain¹

2016

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“…qRT-PCR was performed as previously reported [8]. Reverse transcription was performed, and the cDNA was synthesized from 2 µg of total RNA using an oligo (dT)18 primer and M-MLV reverse transcriptase (Takara Syuzou, Shiga, Japan) for quantitative PCR.…”

Section: Methodsmentioning

confidence: 99%

Corticosteroid Regulates Epithelial Occludin Expression in Nasal Polyps through a MKP-1-Dependent Pathway

Chen

Luo

Han

et al. 2014

ORL

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Objective: To evaluate the regulatory effect of corticosteroid on occludin expression in polyp tissues of chronic rhinosinusitis with nasal polyps (CRSwNP) patients and in vitro. Methods: Twenty CRSwNP patients were enrolled and subjected to prednisone (30 mg/day for 14 days). The expression of occludin in polyp tissues was examined before and after treatment using immunohistochemical staining and quantitative reverse transcription polymerase chain reaction. Moreover, the expression of occludin in polyp-derived epithelial cells (PECs) and human bronchial epithelial cells (BECs) was examined using Western blot analysis in the presence of corticosteroid and/or MKP-1 siRNA, respectively. Results: mRNA and protein expression of occludin in polyp tissues was significantly upregulated after prednisone administration (p < 0.05). Corticosteroids significantly increased MKP-1 and occludin expression in cultured PECs (p < 0.05), and MKP-1 siRNA significantly decreased occludin expression in cultured BECs (p < 0.05). Conclusion: Our findings suggest that corticosteroid can promote epithelial occludin expression in nasal polyps through a MKP-1-dependent pathway. i 2014 S. Karger AG, Basel

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“…14 Briefly, paraffin-embedded human nasal tissues were cut into 4-μm sections and placed onto glass slides. The sections were incubated overnight at 4°C with primary antibodies against GPR17 and CysLT1R with a dilution of 1:200 (Abcam, Cambridge, MA).…”

Section: Ihc Stainingmentioning

confidence: 99%

Expression of cysteinyl leukotriene receptor GPR17 in eosinophilic and non-eosinophilic chronic rhinosinusitis with nasal polyps

Chen

Yang

et al. 2017

Asian Pac J Allergy Immunol

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Background: The cysteinyl leukotrienes (cysLTs) are proinflammatory lipid mediators that act on the type 1 cysLT receptor (CysLT1R) in the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). GPR17, a G protein-coupled orphan receptor with homology to the cysLT receptors, has been proposed as a damage sensor during inflammation. However, the expression and correlation of GPR17 and CysLT1R in eosinophilic CRSwNP (ECRS) and non-eosinophilic CRSwNP (non-ECRS) have not been well investigated.

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Increased neutrophilia in nasal polyps reduces the response to oral corticosteroid therapy

Cited by 249 publications

References 20 publications

Malaysian Nasal Polyps: Eosinophil or Neutrophil-Predominant

Malaysian Nasal Polyps: Eosinophil or Neutrophil-Predominant

Corticosteroid Regulates Epithelial Occludin Expression in Nasal Polyps through a MKP-1-Dependent Pathway

Expression of cysteinyl leukotriene receptor GPR17 in eosinophilic and non-eosinophilic chronic rhinosinusitis with nasal polyps

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