Increased micronuclei and nuclear abnormalities in buccal mucosa and oxidative damage in saliva from patients with chronic and aggressive periodontal diseases

Zamora‐Pérez, Ana Lourdes; Ortiz-García, Yveth Marlene; Lazalde-Ramos, Blanca Patricia; Guerrero-Velázquez, Celia; Gómez‐Meda, Belinda Claudia; Ramírez-Aguilar, M. Á.; Zúñiga‐González, Guillermo Moisés

doi:10.1111/jre.12175

Cited by 41 publications

(38 citation statements)

References 58 publications

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“…Some authors have been demonstrated that individual with periodontal disease exhibited an increase in the frequency of MN, which is directly related to DNA damage [25][26][27]. Similarly, elevated MN frequency has been reported in patients with cancer [28], rheumatoid arthritis [29], autoimmune diseases [30], and premature aging syndrome [31].…”

Section: Periodontitis and Oxs: Nuclear And Oxidative Dna Damagementioning

confidence: 93%

“…This finding could suggest that these variables are associated and can be hypothesized that they could be linked in the development of periodontal disease [25]. The augmented 8-OHdG levels might indicate early oxidative mitochondrial DNA damage [33], and mitochondrial DNA undertakes OxS early than nuclear DNA [42].…”

Section: Periodontitis and Oxs: Nuclear And Oxidative Dna Damagementioning

confidence: 97%

“…ROS generation can occur through different mechanisms such as protein disruption, lipid peroxidation, induction of proinflammatory cytokines, and DNA damage [22]. Therefore, periodontitis is associated with OxS which in turn can lead to nuclear and oxidative DNA damage and thus the formation of MN and 8-OHdG [25].…”

Section: Periodontitis and Oxs: Nuclear And Oxidative Dna Damagementioning

confidence: 99%

“…On the other hand, 8-OHdG level has been studies in oral pathologies, including periodontal disease [25,[32][33][34][35] and oral cancer [36]. As described above, in periodontitis OxS because of the formation of ROS, which is stimulated by neutrophils, produce damage of the bonesupporting tissues.…”

Section: Periodontitis and Oxs: Nuclear And Oxidative Dna Damagementioning

confidence: 99%

“…Elevated levels of 8-OHdG from cancer patients compared with healthy subjects have been observed in lung cancer [37], basal cell carcinoma [38], colorectal cancer [39], bladder cancer [40], and renal cell carcinoma [41]. With respect to periodontitis, published data on oxidative damage to DNA have been reported by many authors around the world who investigated 8-OHdG levels in the saliva of periodontitis patients [4,25,33,34]. These studies demonstrated that salivary levels of 8-OHdG in samples from periodontitis patients were significantly higher than those from periodontally healthy controls and indicated that salivary 8-OHdG levels may be a useful marker for disease activity and may be indirectly reflect disease severity parameters [4,25,33,34].…”

Section: Periodontitis and Oxs: Nuclear And Oxidative Dna Damagementioning

confidence: 99%

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Periodontal Disease and Nuclear and Oxidative DNA Damage

Zamora‐Pérez¹,

Zúñiga‐González²,

Gómez‐Meda³

et al. 2017

Insights Into Various Aspects of Oral Health

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Oral health is an important aspect of the overall health status of an individual. DNA damage has been associated with oral health and dental factors due to the increased of oxidative stress (OxS). DNA damage can produce a wide range of effects on human health. These effects could appear immediately, but others do not become evident much later. Chronic diseases have been study to understand their mechanisms, clinical implications, and the development of secondary disease such as cancer. Periodontitis is one of the most common oral diseases. It is an inflammatory chronic infectious disease, which is characterized by the loss of supporting tissues and tooth loss caused by periodontopathogens and long-term release of reactive oxygen species (ROS); thus, oxidative stress is increased during periodontitis. Oxidative stress can produce DNA damage, including the oxidation of nucleosides, which could cause DNA strand break. This oxidative damage leads the formation of micronuclei (MN) a marker of nuclear damage. Also, oxidative stress increased 8-hydroxy-2′-deoxyguanosine levels which are the most common stable product of oxidative DNA damage.

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Section: Periodontitis and Oxs: Nuclear And Oxidative Dna Damagementioning

confidence: 93%

Section: Periodontitis and Oxs: Nuclear And Oxidative Dna Damagementioning

confidence: 97%

Section: Periodontitis and Oxs: Nuclear And Oxidative Dna Damagementioning

confidence: 99%

Section: Periodontitis and Oxs: Nuclear And Oxidative Dna Damagementioning

confidence: 99%

Section: Periodontitis and Oxs: Nuclear And Oxidative Dna Damagementioning

confidence: 99%

See 3 more Smart Citations

Periodontal Disease and Nuclear and Oxidative DNA Damage

Zamora‐Pérez¹,

Zúñiga‐González²,

Gómez‐Meda³

et al. 2017

Insights Into Various Aspects of Oral Health

View full text Add to dashboard Cite

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Stem Cell‐Niche Engineering via Multifunctional Hydrogel Potentiates Stem Cell Therapies for Inflammatory Bone Loss

et al. 2022

Adv Funct Materials

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Effective therapies capable of simultaneously inhibiting inflammation and promoting bone healing remain to be developed for inflammatory bone disease. Stem cell therapies hold great promise for a variety of diseases, but their translation is hampered by low cell survival, rapid clearance, and limited functional integration of transplanted stem cells in target tissues. Herein, a multifunctional hydrogel-based stem cell niche engineering strategy is reported for the treatment of inflammatory bone loss. By rationally integrating different functional modules, an injectable hydrogel-based stem niche is engineered, which possesses temperature-triggered gelling performance, inflammation/oxidative stress-resolving activity, stem-cell binding and survival-enhancing capacity, and osteogenesis-promoting capability. Using ectomesenchymal stem cells (EMSCs), effectiveness of this functionally advanced synthetic stem cell niche is demonstrated in rats with periodontitis, a representative inflammatory bone loss disease. Synergistic effects of the multifunctional hydrogel and EMSCs are also confirmed, with respect to normalizing the pathological microenvironment and improving alveolar bone regeneration in the periodontal tissue. Mechanistically, inflammation/ oxidative stress-resolving and osteogenic differentiation promoting capacities of the synthetic stem cell niche are mainly achieved by an incorporated nanotherapy via the GDF15/Atf3/c-Fos axis of the MAPK signaling pathway. Besides periodontitis, the newly engineered hydrogel-stem cell therapies are promising for the treatment of other inflammatory bone defects.

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Association between p62 expression and clinicopathological characteristics in oral leukoplakia

Yoshida

Terabe

Nagai

et al. 2019

Clinical & Exp Dental Res

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Objective Oral leukoplakia is keratinized lesions in the buccal mucosa, tongue, and gingiva. It is the most common oral precancerous lesion; oxidative stresses and irrelevant autophagy have been reported to be the cause of oncogenesis. p62, a cytoplasmic protein induced by oxidative stress, is an adaptor protein involved in the formation of protein aggregates and induction and inhibition of autophagy. The inhibition of autophagy induces p62 overexpression and promotes oncogenesis via the oncogenic signaling pathway. The aim of the present study was to elucidate the involvement of intracellular expression of p62 in oral leukoplakia and to address its potential clinical implementation as a biomarker to predict malignant transformation. Material and Methods Fifty samples from subjects with confirmed oral leukoplakia were evaluated by immunohistochemical staining for the expression of p62, 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG), Ki67, and p53. Univariate and multivariate logistic regression analyses were performed to evaluate the association between p62, 8‐OHdG, Ki67, and p53 and clinical characteristics, including epithelial dysplasia. Results Significant associations were observed between p62 expression in the nucleus, p62 aggregation, and epithelial dysplasia (adjusted odds ratio [OR] = 5.75; 95% confidence interval [CI]: [1.28, 26.2]; .024 and OR = 6.16; 95% CI: [1.01, 37.4]; .048, respectively). The expression of p62 in the cytoplasm and the levels of 8‐OHdG, Ki67, and p53 were not significantly associated with epithelial dysplasia. A significant relationship was found between p62 expression in the nucleus and p53 expression (OR = 3.94; 95% CI: [1.14, 13.6]; .031). Conclusions The results suggested that p62 expression in the nucleus and p62 aggregation can be potential markers to predict the malignant transformation of oral leukoplakia.

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Increased micronuclei and nuclear abnormalities in buccal mucosa and oxidative damage in saliva from patients with chronic and aggressive periodontal diseases

Abstract: Our results indicate that subjects with periodontitis (CP or AgP) exhibited an increase in the frequency of MN, NAs and 8-OHdG, which is directly related to DNA damage. In addition, a positive correlation exists between oxidative stress produced by periodontitis disease and MN.

Cited by 41 publications

References 58 publications

Periodontal Disease and Nuclear and Oxidative DNA Damage

Periodontal Disease and Nuclear and Oxidative DNA Damage

Stem Cell‐Niche Engineering via Multifunctional Hydrogel Potentiates Stem Cell Therapies for Inflammatory Bone Loss

Association between p62 expression and clinicopathological characteristics in oral leukoplakia

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