Increased Matrix Metalloproteinase Expression and Activation following Experimental Acute Pancreatitis

Pancreatic Trypsin Increases Matrix Metalloproteinase-9 Accumulation and Activation during Acute Intestinal Ischemia-Reperfusion in the Rat

“…20,23,24 Briefly, SDS-denatured samples under non-reducing conditions were loaded onto 10% poly-acrylamide gels containing 0.1% gelatin (Invitrogen, Carlsbad, CA). After electrophoresis, the proteins were re-natured with Triton X-100 and the gel incubated for 18 hours at 37°C in a developing buffer containing 50 mmol/L Tris-HCl, 150 mmol/L NaCl, 5 mmol/L CaCl 2 , and 0.02% Brj35.…”

“…22 MMP-9 is involved in both the pathogenesis of pancreatitis and in its associated lung injury. 23,24 Despite these findings, the involvement of MMP-9 in intestinal I/R has not been investigated.…”

MMP-9 in Serum Correlates with the Development of Pulmonary Complications in Experimental Acute Pancreatitis

“…The effects of alveolar disruption and protein extravasation into the alveolar space, pathognomic for adult respiratory distress syndrome and pancreatitisassociated lung injury, are promoted by the release of specifi c proteolytic enzymes, matrix metalloproteinases (MMPs), from PMN. Inasmuch as MMP-9 has been evaluated as a local key contributor to pulmonary complications of acute pancreatitis, it has also been identifi ed as a promoter of local disease progression in the pancreas by other groups [7,8] .…”

“…During activation, PMN release an array of deleterious proteases [6] . Others and we previously demonstrated that matrix metalloproteinase-9 (MMP-9), one of these proteases, is not only an important factor for the pathogenesis of acute pancreatitis [7,8] , but additionally is involved in the PMN-mediated pulmonary complications of the disease [6] . In the present study we demonstrate that MMP-9 in serum is a valid parameter to differentiate between the mild and severe course of acute pancreatitis in a standardized experimental model and that MMP-9 in rat serum correlates with and precedes pulmonary injury and complications.…”

Colonic anastomotic strength and matrix metalloproteinase activity in an experimental model of bacterial peritonitis

“…The origin of the increased level of MMPs in healing anastomoses during peritonitis remains a matter of speculation. Inflammatory cells such as PMNs, macrophages and monocytes are sources of various MMPs, and recent studies have described an increase in MMP activity during inflammatory conditions such as pancreatitis, colitis and peritonitis associated with continuous ambulatory peritoneal dialysis 27,28 . Thus, it may be that increased numbers of inflammatory cells, present in the proximity of the healing anastomosis during infectious conditions, are responsible in this respect.…”